Recombinant Anti-CD63 antibody [EPR21151] (ab217345)
![Immunocytochemistry/ Immunofluorescence - Anti-CD63 antibody [EPR21151] (ab217345)](https://www.abcam.com/ps/products/217/ab217345/Images/ab217345-291242-anti-cd63-antibody-epr21151-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CD63 antibody [EPR21151] (ab217345)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21151] to CD63
- Suitable for: WB, ICC/IF
- Reacts with: Mouse
Related conjugates and formulations
Overview
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Product name
Anti-CD63 antibody [EPR21151]
See all CD63 primary antibodies -
Description
Rabbit monoclonal [EPR21151] to CD63 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: bEND.3, RAW 264.7 and NIH/3T3 whole cell lysates; Mouse kidney lysate. ICC/IF: NIH/3T3 and bEND.3 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Human, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21151 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab217345 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/1000. Detects a band of approximately 40-60 kDa (predicted molecular weight: 26 kDa).
|
| ICC/IF | (3) |
1/100.
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| Notes |
|---|
|
WB
1/1000. Detects a band of approximately 40-60 kDa (predicted molecular weight: 26 kDa). |
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ICC/IF
1/100. |
Target
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Function
This antigen is associated with early stages of melanoma tumor progression. May play a role in growth regulation. -
Tissue specificity
Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages. -
Sequence similarities
Belongs to the tetraspanin (TM4SF) family. -
Cellular localization
Cell membrane. Lysosome membrane. Late endosome membrane. Also found in Weibel-Palade bodies of endothelial cells. Located in platelet dense granules. - Information by UniProt
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Database links
- Entrez Gene: 12512 Mouse
- SwissProt: P41731 Mouse
- Unigene: 371552 Mouse
- Unigene: 416079 Mouse
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Alternative names
- Lysosomal associated membrane protein 3 antibody
- CD 63 antibody
- CD63 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-CD63 antibody [EPR21151] (ab217345)
Immunofluorescent analysis of 100% methanol-fixed NIH/3T3 (mouse embryo fibroblast cell line) cells labeling CD63 with ab217345 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in the NIH/3T3 cell line.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
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![Western blot - Anti-CD63 antibody [EPR21151] (ab217345)](https://www.abcam.com/ps/products/217/ab217345/Images/ab217345-291243-anti-cd63-antibody-epr21151-western-blot.jpg)
Western blot - Anti-CD63 antibody [EPR21151] (ab217345)Lanes 1-3 : Anti-CD63 antibody [EPR21151] (ab217345) at 1/2000 dilution
Lane 4 : Anti-CD63 antibody [EPR21151] (ab217345) at 1/1000 dilution
Lane 1 : bEND.3 (mouse brain endothelioma cell line) whole cell lysate at 20 µg
Lane 2 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 20 µg
Lane 3 : Mouse kidney lysate at 20 µg
Lane 4 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 10 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 26 kDa
Observed band size: 40-60 kDa why is the actual band size different from the predicted?Exposure times: Lanes 1-3: 3 minutes; Lane 4: 5 seconds.
Blocking/Dilution buffer: 5% NFDM/TBST.
The molecular masses observed are consistent with what has been described in the literature (PMID: 9890706; PMID: 9790070).
The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.
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![Immunocytochemistry/ Immunofluorescence - Anti-CD63 antibody [EPR21151] (ab217345)](https://www.abcam.com/ps/products/217/ab217345/Images/ab217345-291241-anti-cd63-antibody-epr21151-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD63 antibody [EPR21151] (ab217345)Immunofluorescent analysis of 100% methanol-fixed bEND.3 (mouse brain endothelioma cell line) cells labeling CD63 with ab217345 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in bEND.3 cell line.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
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![Anti-CD63 antibody [EPR21151] (ab217345)](https://www.abcam.com/ps/products/217/ab217345/Images/ab217345-4-benefits-of-recombinant-antibodies.png)
Anti-CD63 antibody [EPR21151] (ab217345)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (71)
ab217345 has been referenced in 71 publications.
- Zhang X et al. BATF2 prevents glioblastoma multiforme progression by inhibiting recruitment of myeloid-derived suppressor cells. Oncogene 40:1516-1530 (2021). PubMed: 33452462
- Ma S et al. Gain-of-function p53 protein transferred via small extracellular vesicles promotes conversion of fibroblasts to a cancer-associated phenotype. Cell Rep 34:108726 (2021). PubMed: 33567287
- Salvany S et al. Microglial recruitment and mechanisms involved in the disruption of afferent synaptic terminals on spinal cord motor neurons after acute peripheral nerve injury. Glia 69:1216-1240 (2021). PubMed: 33386754
- Hu H et al. Role of microRNA-335 carried by bone marrow mesenchymal stem cells-derived extracellular vesicles in bone fracture recovery. Cell Death Dis 12:156 (2021). PubMed: 33542183
- Wu J et al. Analysis of differential expression of long non-coding RNAs in exosomes derived from mature and immature dendritic cells. Mol Med Rep 23:N/A (2021). PubMed: 33313954