Recombinant Anti-CD63 antibody [EPR21151] - BSA and Azide free (ab227892)
![Immunocytochemistry/ Immunofluorescence - Anti-CD63 antibody [EPR21151] - BSA and Azide free (ab227892)](https://www.abcam.com/ps/products/227/ab227892/Images/ab227892-323771-anti-cd63-antibody-epr21151-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CD63 antibody [EPR21151] - BSA and Azide free (ab227892)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21151] to CD63 - BSA and Azide free
- Suitable for: WB, ICC/IF
- Reacts with: Mouse
Related conjugates and formulations
Overview
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Product name
Anti-CD63 antibody [EPR21151] - BSA and Azide free
See all CD63 primary antibodies -
Description
Rabbit monoclonal [EPR21151] to CD63 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: NIH/3T3 cells.
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General notes
ab227892 is the carrier-free version of ab217345.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21151 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab227892 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Detects a band of approximately 40-60 kDa (predicted molecular weight: 26 kDa).
|
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| ICC/IF |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
WB
Use at an assay dependent concentration. Detects a band of approximately 40-60 kDa (predicted molecular weight: 26 kDa). |
|
ICC/IF
Use at an assay dependent concentration. |
Target
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Function
This antigen is associated with early stages of melanoma tumor progression. May play a role in growth regulation. -
Tissue specificity
Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages. -
Sequence similarities
Belongs to the tetraspanin (TM4SF) family. -
Cellular localization
Cell membrane. Lysosome membrane. Late endosome membrane. Also found in Weibel-Palade bodies of endothelial cells. Located in platelet dense granules. - Information by UniProt
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Database links
- Entrez Gene: 12512 Mouse
- SwissProt: P41731 Mouse
- Unigene: 371552 Mouse
- Unigene: 416079 Mouse
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Alternative names
- Lysosomal associated membrane protein 3 antibody
- CD 63 antibody
- CD63 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-CD63 antibody [EPR21151] - BSA and Azide free (ab227892)
Immunofluorescent analysis of 100% methanol-fixed bEND.3 (mouse brain endothelioma cell line) cells labeling CD63 with ab217345 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in bEND.3 cell line.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab217345).
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![Immunocytochemistry/ Immunofluorescence - Anti-CD63 antibody [EPR21151] - BSA and Azide free (ab227892)](https://www.abcam.com/ps/products/227/ab227892/Images/ab227892-291245-anti-cd63-antibody-epr21151-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD63 antibody [EPR21151] - BSA and Azide free (ab227892)Immunofluorescent analysis of 100% methanol-fixed NIH/3T3 (mouse embryo fibroblast cell line) cells labeling CD63 with ab217345 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in the NIH/3T3 cell line.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab217345).
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![Anti-CD63 antibody [EPR21151] - BSA and Azide free (ab227892)](https://www.abcam.com/ps/products/227/ab227892/Images/ab227892-3-benefits-of-recombinant-antibodies.png)
Anti-CD63 antibody [EPR21151] - BSA and Azide free (ab227892)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab227892 has not yet been referenced specifically in any publications.
