Recombinant Anti-CD64 antibody [EPR4624] (ab134073)
![Western blot - Anti-CD64 antibody [EPR4624] (ab134073)](https://www.abcam.com/ps/products/134/ab134073/Images/ab134073-483354-anti-cd64-antibody-epr4624-western-blot-wildtype-thp1-fcgr1a-knockout-thp1-u937-hek293.jpg "Western blot - Anti-CD64 antibody [EPR4624] (ab134073)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4624] to CD64
- Suitable for: WB, IP
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CD64 antibody [EPR4624]
See all CD64 primary antibodies -
Description
Rabbit monoclonal [EPR4624] to CD64 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: THP-1 and U-937 whole cell lysates IP: ab134073 + THP-1 whole cell lysate
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General notes
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4624 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134073 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/10000. Detects a band of approximately 64-78 kDa (predicted molecular weight: 43 kDa).
|
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| IP |
1/30.
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| Notes |
|---|
|
WB
1/10000. Detects a band of approximately 64-78 kDa (predicted molecular weight: 43 kDa). |
|
IP
1/30. |
Target
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Function
High affinity receptor for the Fc region of immunoglobulins gamma. Functions in both innate and adaptive immune responses. -
Tissue specificity
Monocyte/macrophage specific. -
Sequence similarities
Belongs to the immunoglobulin superfamily. FCGR1 family.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains. -
Post-translational
modificationsPhosphorylated on serine residues. -
Cellular localization
Cell membrane. Stabilized at the cell membrane through interaction with FCER1G. - Information by UniProt
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Database links
- Entrez Gene: 2209 Human
- Entrez Gene: 2210 Human
- Omim: 146760 Human
- Omim: 601502 Human
- Omim: 601503 Human
- SwissProt: A6NKC4 Human
- SwissProt: P12314 Human
- SwissProt: Q92637 Human
see all -
Alternative names
- CD 64 antibody
- CD64 antibody
- CD64 antigen antibody
see all
Images
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Western blot - Anti-CD64 antibody [EPR4624] (ab134073)All lanes : Anti-CD64 antibody [EPR4624] (ab134073) at 1/10000 dilution
Lane 1 : Wild-type THP-1 cell lysate
Lane 2 : FCGR1A knockout THP-1 cell lysate
Lane 3 : U937 cell lysate
Lane 4 : HEK-293 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 43 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-CD64 antibody [EPR4624] staining at 1/10000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab134073 was shown to bind specifically to CD64. A band was observed at 45-50/55-80 kDa in wild-type THP-1 cell lysates with no signal observed at this size in FCGR1A knockout cell line ab275843 (knockout cell lysate ab275817). To generate this image, wild-type and FCGR1A knockout THP-1 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
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![Immunoprecipitation - Anti-CD64 antibody [EPR4624] (ab134073)](https://www.abcam.com/ps/products/134/ab134073/Images/ab134073-5-anti-cd64-antibody-epr4624-immunoprecipitation-thp1-human.jpg)
Immunoprecipitation - Anti-CD64 antibody [EPR4624] (ab134073)Purified ab134073 at 1:30 dilution (2µg) immunoprecipitating CD64 in THP-1 whole cell lysate.
Lane 1 (input): THP-1 (Human monocytic leukemia monocyte) whole cell lysate 10µg.
Lane 2 (+): ab134073 + THP-1 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab134073 in THP-1 whole cell lysate.
VeriBlot for IP Detection Reagent (HRP)™(ab131366) (1:1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: Glycosylated form: 64-78kDa; Non-glycosylated form: 39-43kDa. -
![Western blot - Anti-CD64 antibody [EPR4624] (ab134073)](https://www.abcam.com/ps/products/134/ab134073/Images/ab134073-478309-anti-cd64-antibody-epr4624-westernblot-human.jpg)
Western blot - Anti-CD64 antibody [EPR4624] (ab134073)All lanes : Anti-CD64 antibody [EPR4624] (ab134073) at 1/10000 dilution (Purified)
Lane 1 : THP-1 (Human monocytic leukemia monocyte) whole cell lysate
Lane 2 : U-937 (Human histiocytic lymphoma monocyte) whole cell lysate
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 43 kDaGlycosylated form: 64-78kDa; Non-glycosylated form: 39-43kDa
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![Anti-CD64 antibody [EPR4624] (ab134073)](https://www.abcam.com/ps/products/134/ab134073/Images/ab134073-2-benefits-of-recombinant-antibodies.png)
Anti-CD64 antibody [EPR4624] (ab134073)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab134073 has been referenced in 1 publication.
- Duffney PF et al. Ozone-derived oxysterols impair lung macrophage phagocytosis via adduction of some phagocytosis receptors. J Biol Chem 295:12727-12738 (2020). PubMed: 32690608