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  1. Link

    products/primary-antibodies/cd68-antibody-ed1-ab31630.pdf

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Immunology Cell Type Markers CD Myeloid Cells
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Anti-CD68 antibody [ED1] (ab31630)

  • Datasheet
  • SDS
Reviews (20)Q&A (4)References (301)

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Flow Cytometry (Intracellular) - Anti-CD68 antibody [ED1] (ab31630)

    Key features and details

    • Mouse monoclonal [ED1] to CD68
    • Suitable for: Flow Cyt (Intra)
    • Reacts with: Rat
    • Isotype: IgG1

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    Overview

    • Product name

      Anti-CD68 antibody [ED1]
      See all CD68 primary antibodies
    • Description

      Mouse monoclonal [ED1] to CD68
    • Host species

      Mouse
    • Specificity

      Ab31630 recognises a single chain glycoprotein of 110kD that is expressed predominantly on the lysosomal membrane of myeloid cells. Weak cell surface expression also occurs. The antigen is expressed by the majority of tissue macrophages and weakly by peripheral blood granulocytes. Studies have shown that the antigen recognised by ED1 is the rat homologue of human CD68.
    • Tested applications

      Suitable for: Flow Cyt (Intra)more details
    • Species reactivity

      Reacts with: Rat
    • Immunogen

      Tissue, cells or virus corresponding to Rat CD68. Rat spleen cell.

    • Positive control

      • Flow Cyt (Intra): Rat peritoneal macrophages.
    • General notes

      This product should be stored undiluted.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.40
      Preservative: 0.09% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • Purity

      Protein A purified
    • Purification notes

      Purified from tissue culture supernatant.
    • Clonality

      Monoclonal
    • Clone number

      ED1
    • Myeloma

      Sp2/0-Ag14
    • Isotype

      IgG1
    • Research areas

      • Immunology
      • Cell Type Markers
      • CD
      • Myeloid Cells
      • Microbiology
      • Organism
      • Virus
      • RNA Virus
      • ssRNA positive strand virus
      • SARS Coronavirus
      • Signal Transduction
      • Antibodies
      • cd68
      • Neuroscience
      • Development

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Conjugation kits

      • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
      • APC Conjugation Kit - Lightning-Link® (ab201807)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Recombinant Protein

      • Recombinant human CD68 protein (Fc Chimera Active) (ab214829)
    • Related Products

      • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)
      • Alexa Fluor® 488 Anti-CD68 antibody [FA-11] (ab201844)
      • Alexa Fluor® 647 Anti-CD68 antibody [FA-11] (ab201845)
      • Anti-TMEM119 antibody [28-3] - Microglial marker (ab209064)
      • Anti-CD68 antibody [FA-11] (ab53444)
      • Anti-CD11b antibody [M1/70] (ab8878)
      • Anti-F4/80 antibody [F4/80] - Macrophage Marker (ab90247)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab31630 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt (Intra)
    1/50 - 1/100.

    Use 10µl of the suggested working dilution to label 106 cells in 100µl. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

    Notes
    Flow Cyt (Intra)
    1/50 - 1/100.

    Use 10µl of the suggested working dilution to label 106 cells in 100µl. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

    Target

    • Function

      Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
    • Tissue specificity

      Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.
    • Sequence similarities

      Belongs to the LAMP family.
    • Post-translational
      modifications

      N- and O-glycosylated.
    • Cellular localization

      Cell membrane and Endosome membrane. Lysosome membrane.
    • Target information above from: UniProt accession P34810 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 287435 Rat
      • Alternative names

        • CD 68 antibody
        • CD68 antibody
        • CD68 antigen antibody
        • CD68 molecule antibody
        • CD68_HUMAN antibody
        • DKFZp686M18236 antibody
        • gp11 antibody
        • Gp110 antibody
        • LAMP4 antibody
        • Macrophage antigen CD68 (microsialin) antibody
        • MACROPHAGE ANTIGEN CD68 antibody
        • Macrosialin antibody
        • SCARD1 antibody
        • Scavenger receptor class D member 1 antibody
        see all

      Images

      • Flow Cytometry (Intracellular) - Anti-CD68 antibody [ED1] (ab31630)
        Flow Cytometry (Intracellular) - Anti-CD68 antibody [ED1] (ab31630)

        Staining of rat peritoneal macrophages with Alexa Fluor®647 conjugated mouse anti rat CD68, clone ED1 (ab31630, red filled histogram) following permeabilization with Leucoperm. 

         

         

      Protocols

      • Flow cytometry protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (301)

      Publishing research using ab31630? Please let us know so that we can cite the reference in this datasheet.

      ab31630 has been referenced in 301 publications.

      • Tian F & Zhang Y Overexpression of SERCA2a Alleviates Cardiac Microvascular Ischemic Injury by Suppressing Mfn2-Mediated ER/Mitochondrial Calcium Tethering. Front Cell Dev Biol 9:636553 (2021). PubMed: 33869181
      • Bandoni RL  et al. Cholinergic stimulation with pyridostigmine modulates a heart-spleen axis after acute myocardial infarction in spontaneous hypertensive rats. Sci Rep 11:9563 (2021). PubMed: 33953291
      • Chen ML  et al. Inhibition of miR-331-3p and miR-9-5p ameliorates Alzheimer's disease by enhancing autophagy. Theranostics 11:2395-2409 (2021). PubMed: 33500732
      • Irastorza-Lorenzo A  et al. Evaluation of Marine Agarose Biomaterials for Tissue Engineering Applications. Int J Mol Sci 22:N/A (2021). PubMed: 33672027
      • Yu Q  et al. Dexmedetomidine suppresses the development of abdominal aortic aneurysm by downregulating the mircoRNA-21/PDCD 4 axis. Int J Mol Med 47:N/A (2021). PubMed: 33786608
      View all Publications for this product

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-10 of 24 Abreviews or Q&A

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD68 antibody [ED1]

      Average
      Abreviews
      Abreviews
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (heart)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate
      Permeabilization
      Yes - 0.1% triton-x
      Specification
      heart
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
      Fixative
      Formaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      Submitted Jun 25 2020

      Western blot abreview for Anti-CD68 antibody [ED1]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Tissue lysate - whole (Spleen)
      Gel Running Conditions
      Reduced Denaturing (4-20%)
      Loading amount
      20 µg
      Specification
      Spleen
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      DR. Sergi Bayod

      Verified customer

      Submitted Oct 28 2019

      Immunohistochemistry free floating abreview for Anti-CD68 antibody [ED1]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry free floating
      Sample
      Mouse Tissue sections (Brain)
      Specification
      Brain
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      DR. Alexandre Magno

      Verified customer

      Submitted Jul 31 2018

      Immunohistochemistry (Frozen sections) abreview for Anti-CD68 antibody [ED1]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Mouse Tissue sections (Tumor)
      Permeabilization
      Yes - 100% Acetone
      Specification
      Tumor
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
      Fixative
      10% normal buffered formalin
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      Submitted Aug 02 2017

      Immunohistochemistry (Frozen sections) abreview for Anti-CD68 antibody [ED1]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Rat Tissue sections (Lung)
      Permeabilization
      Yes - 80% Acetone
      Specification
      Lung
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
      Fixative
      Paraformaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      Submitted Aug 02 2017

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD68 antibody [ED1]

      Average
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (Skin)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
      Permeabilization
      No
      Specification
      Skin
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
      Fixative
      10% normal buffered formalin
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      Submitted May 01 2017

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD68 antibody [ED1]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Rat Tissue sections (spleen)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
      Permeabilization
      No
      Specification
      spleen
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
      Fixative
      Paraformaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      Submitted Apr 17 2017

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD68 antibody [ED1]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (skin)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: EDTA-Buffer pH 9,0
      Permeabilization
      No
      Specification
      skin
      Blocking step
      Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
      Fixative
      Formaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      Submitted Jan 19 2017

      Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-CD68 antibody [ED1]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (PFA perfusion fixed frozen sections)
      Sample
      Rat Tissue sections (Injured spinal cord)
      Antigen retrieval step
      None
      Permeabilization
      No
      Specification
      Injured spinal cord
      Blocking step
      Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 26°C
      Fixative
      Paraformaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      Submitted Sep 14 2016

      Immunocytochemistry/ Immunofluorescence abreview for Anti-CD68 antibody [ED1]

      Inconclusive
      Abreviews
      Abreviews
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Mouse Cell (Microglia)
      Permeabilization
      Yes - Triton X100
      Specification
      Microglia
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Fixative
      Paraformaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Ray Valued Customer

      Verified customer

      Submitted Feb 01 2016

      1-10 of 24 Abreviews or Q&A

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