Recombinant Anti-CD68 antibody [SP251], prediluted (ab228185)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [SP251], prediluted (ab228185)](https://www.abcam.com/ps/products/228/ab228185/Images/ab228185-291922-anti-cd68-antibody-sp251-prediluted-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [SP251], prediluted (ab228185)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP251] to CD68, prediluted
- Suitable for: IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CD68 antibody [SP251], prediluted
See all CD68 primary antibodies -
Description
Rabbit monoclonal [SP251] to CD68, prediluted -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human tonsil tissue.
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: Tris buffered saline, 1% BSA -
Concentration information loading... -
Purity
Protein A/G purified -
Purification notes
Purified from TCS by protein A/G. -
Clonality
Monoclonal -
Clone number
SP251 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-CD68 antibody [SP251] (ab192847)
- Alexa Fluor® 488 Anti-CD68 antibody [SP251] (ab309940)
- Alexa Fluor® 647 Anti-CD68 antibody [SP251] (ab310307)
- Alexa Fluor® 594 Anti-CD68 antibody [SP251] (ab310770)
- Alexa Fluor® 555 Anti-CD68 antibody [SP251] (ab312297)
- Alexa Fluor® 568 Anti-CD68 antibody [SP251] (ab312792)
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab228185 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/1.
Ready to use. Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes. Primary antibody incubation for 10 minutes at room temperature. |
| Notes |
|---|
|
IHC-P
1/1. Ready to use. Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes. Primary antibody incubation for 10 minutes at room temperature. |
Target
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Function
Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells. -
Tissue specificity
Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites. -
Sequence similarities
Belongs to the LAMP family. -
Post-translational
modificationsN- and O-glycosylated. -
Cellular localization
Cell membrane and Endosome membrane. Lysosome membrane. - Information by UniProt
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Database links
- Entrez Gene: 968 Human
- Omim: 153634 Human
- SwissProt: P34810 Human
- Unigene: 647419 Human
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Alternative names
- CD 68 antibody
- CD68 antibody
- CD68 antigen antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [SP251], prediluted (ab228185)
Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD68 using ab228185 in immunohistochemical analysis.
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![Anti-CD68 antibody [SP251], prediluted (ab228185)](https://www.abcam.com/ps/products/228/ab228185/Images/ab228185-2-benefits-of-recombinant-antibodies.png)
Anti-CD68 antibody [SP251], prediluted (ab228185)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab228185 has not yet been referenced specifically in any publications.