For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    products/primary-antibodies/cd73-antibody-4g6e3-ab202122.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Cardiovascular Hypoxia Associated Proteins
Share by email
Validated using a knockout cell line

Anti-CD73 antibody [4G6E3] (ab202122)

  • Datasheet
Reviews (1) Submit a question References (3)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-CD73 antibody [4G6E3] (ab202122)
  • Flow Cytometry - Anti-CD73 antibody [4G6E3] (ab202122)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD73 antibody [4G6E3] (ab202122)
  • Western blot - Anti-CD73 antibody [4G6E3] (ab202122)
  • Western blot - Anti-CD73 antibody [4G6E3] (ab202122)
  • Western blot - Anti-CD73 antibody [4G6E3] (ab202122)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD73 antibody [4G6E3] (ab202122)

Key features and details

  • Mouse monoclonal [4G6E3] to CD73
  • Suitable for: Flow Cyt, IHC-P, WB
  • Knockout validated
  • Reacts with: Human
  • Isotype: IgG1

You may also be interested in

Secondary
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)
Assay
Product image
5’-Nucleotidase (CD73) Activity Assay Kit (Colorimetric) (ab235945)
Knockout
Product image
Human NT5E knockout A-431 cell line (ab261895)

View more associated products

Overview

  • Product name

    Anti-CD73 antibody [4G6E3]
    See all CD73 primary antibodies
  • Description

    Mouse monoclonal [4G6E3] to CD73
  • Host species

    Mouse
  • Tested applications

    Suitable for: Flow Cyt, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment corresponding to Human CD73 aa 1-250. Expressed in E. Coli.
    Database link: P21589

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Flow Cytometry: A431 cells; WB: A375, and A431 cell lysates; IHC-P: Human endometrial cancer and Human esophageal cancer tissues.
  • General notes

    This product was changed from ascites to supernatant. Lot no’s high than GR287343-13 are from Tissue Culture Supernatant

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    4G6E3
  • Isotype

    IgG1
  • Research areas

    • Cardiovascular
    • Hypoxia
    • Associated Proteins
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Leukocyte recruitment
    • Other

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • KO cell lines

    • Human NT5E knockout A-431 cell line (ab261895)
  • KO cell lysates

    • Human NT5E knockout A-431 cell lysate (ab261704)
  • Positive Controls

    • A-431 whole cell lysate (ab7909)
  • Recombinant Protein

    • Recombinant mouse CD73 protein (His tag) (ab223023)
  • Related Products

    • Recombinant Human CD73 protein (ab151934)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab202122 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-P (1)
1/200 - 1/1000.
WB
1/500 - 1/2000.
Notes
Flow Cyt
1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-P
1/200 - 1/1000.
WB
1/500 - 1/2000.

Target

  • Function

    Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.
  • Involvement in disease

    Defects in NT5E are the cause of calcification of joints and arteries (CAJA) [MIM:211800]. A condition characterized by adult-onset calcification of the lower extremity arteries, including the iliac, femoral and tibial arteries, and hand and foot capsule joints. Age of onset has been reported as early as the second decade of life, usually involving intense joint pain or calcification in the hands.
  • Sequence similarities

    Belongs to the 5'-nucleotidase family.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession P21589 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 281363 Cow
    • Entrez Gene: 4907 Human
    • Entrez Gene: 23959 Mouse
    • Entrez Gene: 58813 Rat
    • Omim: 129190 Human
    • SwissProt: Q05927 Cow
    • SwissProt: P21589 Human
    • SwissProt: Q61503 Mouse
    • SwissProt: P21588 Rat
    • Unigene: 153952 Human
    • Unigene: 244235 Mouse
    • Unigene: 40132 Rat
    see all
  • Alternative names

    • 5' NT antibody
    • 5' nucleotidase (CD73) antibody
    • 5' nucleotidase precursor antibody
    • 5' nucleotidase, ecto antibody
    • 5' nucleotidase, ecto (CD73) antibody
    • 5'-NT antibody
    • 5'-nucleotidase antibody
    • 5NTD_HUMAN antibody
    • CD73 antibody
    • CD73 antigen antibody
    • E5NT antibody
    • Ecto 5' nucleotidase antibody
    • Ecto-5'-nucleotidase antibody
    • eN antibody
    • eNT antibody
    • NT antibody
    • NT5 antibody
    • NT5E antibody
    • NTE antibody
    • Purine 5 Prime Nucleotidase antibody
    see all

Images

  • Western blot - Anti-CD73 antibody [4G6E3] (ab202122)
    Western blot - Anti-CD73 antibody [4G6E3] (ab202122)
    All lanes : Anti-CD73 antibody [4G6E3] (ab202122) at 1/500 dilution

    Lane 1 : Wild-type A-375 cell lysate
    Lane 2 : NT5E knockout A-375 cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Observed band size: 65 kDa why is the actual band size different from the predicted?



    False colour image of Western blot: Anti-CD73 antibody [4G6E3] staining at 1/500 dilution, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (ab181602) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab202122 was shown to bind specifically to CD73. A band was observed at 65-70 kDa in wild-type A-375 cell lysates with no signal observed at this size in NT5E knockout cell line ab281591 (knockout cell lysate ab282943). A cross-reactive band is observed at 65 kDa in all samples. To generate this image, wild-type and NT5E knockout A-375 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) at 1/20000 dilution.

  • Flow Cytometry - Anti-CD73 antibody [4G6E3] (ab202122)
    Flow Cytometry - Anti-CD73 antibody [4G6E3] (ab202122)

    Flow cytometric analysis of A431 cells using ab202122 at 1/200 (green) and negative control (red).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD73 antibody [4G6E3] (ab202122)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD73 antibody [4G6E3] (ab202122)

    Immunohistochemical analysis of paraffin-embedded Human esophageal cancer tissue labeling CD73 with ab202122 at 1/200 with DAB staining.

  • Western blot - Anti-CD73 antibody [4G6E3] (ab202122)
    Western blot - Anti-CD73 antibody [4G6E3] (ab202122)
    All lanes : Anti-CD73 antibody [4G6E3] (ab202122) at 1/1000 dilution

    Lane 1 : Wild-type A-431 whole cell lysate
    Lane 2 : NT5E knockout A-431 (Human epidermoid carcinoma cell line) whole cell lysate
    Lane 3 : A375 (Human malignant melanoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.


    Lanes 1 - 3: Merged signal (red and green). Green - ab202122 observed at 63 kDa. Red - loading control, ab181602, observed at 37 kDa.

    ab202122 was shown to recognize NT5E in wild-type A-431 cells as signal was lost at the expected MW in NT5E knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and NT5E knockout samples were subjected to SDS-PAGE. Ab202122 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-CD73 antibody [4G6E3] (ab202122)
    Western blot - Anti-CD73 antibody [4G6E3] (ab202122)
    Anti-CD73 antibody [4G6E3] (ab202122) at 1/500 dilution + A431 cell lysate
  • Western blot - Anti-CD73 antibody [4G6E3] (ab202122)
    Western blot - Anti-CD73 antibody [4G6E3] (ab202122)
    Anti-CD73 antibody [4G6E3] (ab202122) at 1/500 dilution + Human CD73 (AA: 30-250) recombinant protein


    Expected MW is 26.6 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD73 antibody [4G6E3] (ab202122)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD73 antibody [4G6E3] (ab202122)

    Immunohistochemical analysis of paraffin-embedded Human endometrial cancer tissue labeling CD73 with ab202122 at 1/200 with DAB staining.

Protocols

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (3)

Publishing research using ab202122? Please let us know so that we can cite the reference in this datasheet.

ab202122 has been referenced in 3 publications.

  • Li B  et al. The MSC-Derived Exosomal lncRNA H19 Promotes Wound Healing in Diabetic Foot Ulcers by Upregulating PTEN via MicroRNA-152-3p. Mol Ther Nucleic Acids 19:814-826 (2020). PubMed: 31958697
  • Pan C  et al. Pretreatment with human urine-derived stem cells protects neurological function in rats following cardiopulmonary resuscitation after cardiac arrest. Exp Ther Med 20:112 (2020). PubMed: 32989390
  • Yu L  et al. Exosomes derived from microRNA-199a-overexpressing mesenchymal stem cells inhibit glioma progression by down-regulating AGAP2. Aging (Albany NY) 11:5300-5318 (2019). PubMed: 31386624

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD73 antibody [4G6E3]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate Buffer pH6.0
Permeabilization
No
Specification
Kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 28 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2023 Abcam plc. All rights reserved.