Recombinant Anti-CD8 alpha antibody [EP1150Y] (ab93278)
![Immunocytochemistry/ Immunofluorescence - Anti-CD8 alpha antibody [EP1150Y] (ab93278)](https://www.abcam.com/ps/products/93/ab93278/Images/ab93278-7-anti-cd8-alpha-antibody-ep1150y-immunocytochemistry-molt4-human.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CD8 alpha antibody [EP1150Y] (ab93278)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1150Y] to CD8 alpha
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-CD8 alpha antibody [EP1150Y]
See all CD8 alpha primary antibodies -
Description
Rabbit monoclonal [EP1150Y] to CD8 alpha -
Host species
Rabbit -
Specificity
The immunogen sequence for this antibody has no significant homology with mouse and rat CD alpha and beta. This product showed specific staining in lymphocytes, although we observed background staining in some epithelial cells tested. -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to Human CD8 alpha aa 200 to the C-terminus (C terminal).
Database link: P01732 -
Positive control
- WB: THP-1 cell lysate. ICC/IF: MOLT-4 cells
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1150Y -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab93278 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 26 kDa.
For unpurified use at 1/1000 - 1/10000. |
|
| ICC/IF |
1/500.
For unpurified use at 1/50 - 1/100. |
| Notes |
|---|
|
WB
1/1000. Predicted molecular weight: 26 kDa. For unpurified use at 1/1000 - 1/10000. |
|
ICC/IF
1/500. For unpurified use at 1/50 - 1/100. |
Target
-
Function
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. -
Involvement in disease
Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsAll of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
-
Database links
- Entrez Gene: 925 Human
- Omim: 186910 Human
- SwissProt: P01732 Human
- Unigene: 85258 Human
-
Alternative names
- alpha polypeptide (p32) antibody
- CD_antigen=CD8a antibody
- CD8 antibody
see all
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-CD8 alpha antibody [EP1150Y] (ab93278)
Immunocytochemistry analysis of MOLT-4 (human lymphoblastic leukemia T lymphoblast) labeling CD8 alpha with purified ab93278 at 1/250 dilution (9 µg/ml). Cells were fixed with 100% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.21 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
Negative control: PBS instead of the primary antibody. -
![Western blot - Anti-CD8 alpha antibody [EP1150Y] (ab93278)](https://www.abcam.com/ps/products/93/ab93278/Images/ab93278-108373-anti-cd8-antibody-ep1150y-western-blot.jpg)
Western blot - Anti-CD8 alpha antibody [EP1150Y] (ab93278)Anti-CD8 alpha antibody [EP1150Y] (ab93278) at 1/1000 dilution (unpurified) + THP-1 cell lysate at 10 µg
Predicted band size: 26 kDa -
![Anti-CD8 alpha antibody [EP1150Y] (ab93278)](https://www.abcam.com/ps/products/93/ab93278/Images/ab93278-8-benefits-of-recombinant-antibodies.png)
Anti-CD8 alpha antibody [EP1150Y] (ab93278)
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (45)
ab93278 has been referenced in 45 publications.
- Liu J et al. Multicenter phase II trial of Camrelizumab combined with Apatinib and Eribulin in heavily pretreated patients with advanced triple-negative breast cancer. Nat Commun 13:3011 (2022). PubMed: 35641481
- Lei X et al. Mitochondrial fission induces immunoescape in solid tumors through decreasing MHC-I surface expression. Nat Commun 13:3882 (2022). PubMed: 35794100
- Wu J et al. SPOP promotes cervical cancer progression by inducing the movement of PD-1 away from PD-L1 in spatial localization. J Transl Med 20:384 (2022). PubMed: 36042498
- Huo X et al. Identification of Novel Immunologic Checkpoint Gene Prognostic Markers for Ovarian Cancer. J Oncol 2022:8570882 (2022). PubMed: 36157232
- Zhu J et al. Photothermal Nano-Vaccine Promoting Antigen Presentation and Dendritic Cells Infiltration for Enhanced Immunotherapy of Melanoma via Transdermal Microneedles Delivery. Research (Wash D C) 2022:9816272 (2022). PubMed: 36157510