Recombinant Anti-CD8 alpha antibody [EPR20305] (ab209775)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [EPR20305] (ab209775)](https://www.abcam.com/ps/products/209/ab209775/Images/ab209775-280354-anti-cd8-alpha-antibody-epr20305-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [EPR20305] (ab209775)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20305] to CD8 alpha
- Suitable for: IHC-P, WB
- Reacts with: Mouse
Related conjugates and formulations
Overview
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Product name
Anti-CD8 alpha antibody [EPR20305]
See all CD8 alpha primary antibodies -
Description
Rabbit monoclonal [EPR20305] to CD8 alpha -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse lymph node, thymus and spleen lysates. IHC-P: Mouse spleen and thymus tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20305 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab209775 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (5) |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| WB | (1) |
1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 27 kDa).
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| Notes |
|---|
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IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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WB
1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 27 kDa). |
Target
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Function
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. -
Involvement in disease
Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsAll of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 12525 Mouse
- SwissProt: P01731 Mouse
- Unigene: 1858 Mouse
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Alternative names
- alpha polypeptide (p32) antibody
- CD_antigen=CD8a antibody
- CD8 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [EPR20305] (ab209775)
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD8 alpha with ab209775 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous staining on T cells around peripheral artery lymphatic sheath of mouse spleen (PMID: 23482450). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [EPR20305] (ab209775)](https://www.abcam.com/ps/products/209/ab209775/Images/ab209775-280353-anti-cd8-alpha-antibody-epr20305-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [EPR20305] (ab209775)Immunohistochemical analysis of paraffin-embedded mouse thymus tissue labeling CD8 alpha with ab209775 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous staining on mouse thymus. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Western blot - Anti-CD8 alpha antibody [EPR20305] (ab209775)](https://www.abcam.com/ps/products/209/ab209775/Images/ab209775-280352-anti-cd8-alpha-antibody-epr20305-western-blot.jpg)
Western blot - Anti-CD8 alpha antibody [EPR20305] (ab209775)All lanes : Anti-CD8 alpha antibody [EPR20305] (ab209775) at 1/1000 dilution
Lane 1 : Mouse lymph node lysate
Lane 2 : Mouse thymus lysate
Lane 3 : Mouse spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 27 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 10 seconds; Lane 2: 1 second; Lane 3: 3 minutes.
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![Anti-CD8 alpha antibody [EPR20305] (ab209775)](https://www.abcam.com/ps/products/209/ab209775/Images/ab209775-4-benefits-of-recombinant-antibodies.png)
Anti-CD8 alpha antibody [EPR20305] (ab209775)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (38)
ab209775 has been referenced in 38 publications.
- Okada R et al. Near-infrared photoimmunotherapy targeting human-EGFR in a mouse tumor model simulating current and future clinical trials. EBioMedicine 67:103345 (2021). PubMed: 33933782
- You G et al. B7-H3×4-1BB bispecific antibody augments antitumor immunity by enhancing terminally differentiated CD8+ tumor-infiltrating lymphocytes. Sci Adv 7:N/A (2021). PubMed: 33523913
- Wang F et al. Discovery of a new inhibitor targeting PD-L1 for cancer immunotherapy. Neoplasia 23:281-293 (2021). PubMed: 33529880
- Kwon B et al. Effect of Cone of Pinus densiflora on DNCB-Induced Allergic Contact Dermatitis-Like Skin Lesion in Balb/c Mice. Nutrients 13:N/A (2021). PubMed: 33806628
- Liu C et al. Discovery of a novel, potent and selective small-molecule inhibitor of PD-1/PD-L1 interaction with robust in vivo anti-tumour efficacy. Br J Pharmacol 178:2651-2670 (2021). PubMed: 33768523