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    products/primary-antibodies/cd8-alpha-antibody-epr22483-288-ab245118.pdf

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Immunology Adaptive Immunity T Cells Cytotoxic Cells
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RecombinantRabMAb

Recombinant Anti-CD8 alpha antibody [EPR22483-288] (ab245118)

  • Datasheet
  • SDS
  • Certificate of Compliance
Reviews (1) Submit a question References (1)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [EPR22483-288] (ab245118)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [EPR22483-288] (ab245118)
  • Flow Cytometry - Anti-CD8 alpha antibody [EPR22483-288] (ab245118)
  • Immunoprecipitation - Anti-CD8 alpha antibody [EPR22483-288] (ab245118)
  • Anti-CD8 alpha antibody [EPR22483-288] (ab245118)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22483-288] to CD8 alpha
  • Suitable for: IHC-P, Flow Cyt, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Recombinant Human CD8 alpha protein (ab114161)
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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
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Anti-KLRG1 antibody (ab235951)

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Overview

  • Product name

    Anti-CD8 alpha antibody [EPR22483-288]
    See all CD8 alpha primary antibodies
  • Description

    Rabbit monoclonal [EPR22483-288] to CD8 alpha
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, Flow Cyt, IPmore details
    Unsuitable for: ICC/IF or WB
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human colon and tonsil tissue. Flow Cyt: Human peripheral blood mononuclear cells. IP: CD8 alpha IP in human thymus lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR22483-288
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • T Cells
    • Cytotoxic Cells
    • Immunology
    • Adaptive Immunity
    • T Cells
    • CD
    • Cancer
    • Tumor immunology
    • CD markers

Associated products

  • Alternative Versions

    • Anti-CD8 alpha antibody [EPR22483-288] - BSA and Azide free (ab245830)
  • Compatible Secondaries

    • VeriBlot for IP Detection Reagent (HRP) (ab131366)
  • Immunohistochemistry reagents

    • Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human CD8 alpha protein (ab114161)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab245118 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P (1)
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt
1/400.
IP
1/30.
Notes
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt
1/400.
IP
1/30.
Application notes
Is unsuitable for ICC/IF or WB.

Target

  • Function

    Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
  • Involvement in disease

    Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
  • Sequence similarities

    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications

    All of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.
  • Cellular localization

    Secreted and Cell membrane.
  • Target information above from: UniProt accession P01732 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 925 Human
    • Omim: 186910 Human
    • SwissProt: P01732 Human
    • Unigene: 85258 Human
    • Alternative names

      • alpha polypeptide (p32) antibody
      • CD_antigen=CD8a antibody
      • CD8 antibody
      • CD8 antigen alpha polypeptide antibody
      • CD8 antigen alpha polypeptide (p32) antibody
      • CD8 antigen, alpha polypeptide (p32) antibody
      • CD8a antibody
      • CD8A antigen antibody
      • CD8A molecule antibody
      • CD8A_HUMAN antibody
      • Leu2 antibody
      • Leu2 T lymphocyte antigen antibody
      • Ly 2 antibody
      • Ly 35 antibody
      • Ly B antibody
      • Ly2 antibody
      • Ly3 antibody
      • Ly35 antibody
      • LyB antibody
      • Lyt 2.1 lymphocyte differentiation antigen (AA at 100) antibody
      • LYT3 antibody
      • MAL antibody
      • OKT8 T cell antigen antibody
      • OTTHUMP00000160760 antibody
      • OTTHUMP00000160764 antibody
      • OTTHUMP00000203528 antibody
      • OTTHUMP00000203721 antibody
      • p32 antibody
      • T cell antigen Leu2 antibody
      • T cell co receptor antibody
      • T lymphocyte differentiation antigen T8/Leu 2 antibody
      • T-cell surface glycoprotein CD8 alpha chain antibody
      • T-cell surface glycoprotein Lyt 2 antibody
      • T-lymphocyte differentiation antigen T8/Leu-2 antibody
      • T8 T cell antigen antibody
      • T8/Leu-2 T-lymphocyte differentiation antigen antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [EPR22483-288] (ab245118)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [EPR22483-288] (ab245118)

      Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD8 alpha with ab245118 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on the T cell zone of human tonsil (PMID: 16504163) is observed. Counterstained with hematoxylin.
      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
      Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [EPR22483-288] (ab245118)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [EPR22483-288] (ab245118)

      Immunohistochemical analysis of paraffin-embedded human colon tissue labeling CD8 alpha with ab245118 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on lymphocytes of human colon (PMID: 26310568) is observed. Counterstained with hematoxylin.
      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
      Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    • Flow Cytometry - Anti-CD8 alpha antibody [EPR22483-288] (ab245118)
      Flow Cytometry - Anti-CD8 alpha antibody [EPR22483-288] (ab245118)

      Flow cytometric analysis of human peripheral blood mononuclear cells (PBMC) labeling CD8 alpha with ab245118 at 1/400 (right panel) compared with a Rabbit monoclonal IgG (ab172730) (left panel). Goat anti rabbit IgG (Dylight® 488, ab98462) at 1/2000 dilution was used as the secondary antibody.

      Cells were stained with rabbit IgG (Left) or anti-CD8 alpha RabMab (Right). Then stained with Alexa Fluor® 647-conjugated anti-CD4. The expressions of CD4 and CD8 alpha are mutually exclusive.

      Gated on viable lymphocytes.

    • Immunoprecipitation - Anti-CD8 alpha antibody [EPR22483-288] (ab245118)
      Immunoprecipitation - Anti-CD8 alpha antibody [EPR22483-288] (ab245118)

      CD8 alpha was immunoprecipitated from 0.35 mg human thymus lysate with ab245118 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab245118 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

      Lane 1: Human thymus lysate 10 µg (Input).
      Lane 2: ab245118 IP in human thymus lysate.
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab245118 in human thymus lysate.

      Blocking/Dilution buffer: 5% NFDM/TBST.
      Exposure time: 30 seconds.

    • Anti-CD8 alpha antibody [EPR22483-288] (ab245118)
      Anti-CD8 alpha antibody [EPR22483-288] (ab245118)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (1)

    Publishing research using ab245118? Please let us know so that we can cite the reference in this datasheet.

    ab245118 has been referenced in 1 publication.

    • Li S  et al. A risk signature with inflammatory and immune cells infiltration predicts survival and efficiency of chemotherapy in gastric cancer. Int Immunopharmacol 96:107589 (2021). PubMed: 34162126

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD8 alpha antibody [EPR22483-288]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Monkey Tissue sections (Spleen)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH9
    Permeabilization
    No
    Specification
    Spleen
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. siyuan yan

    Verified customer

    Submitted Dec 02 2022

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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