Recombinant Anti-CD81 antibody [EPR21916] (ab219209)
![Immunocytochemistry/ Immunofluorescence - Anti-CD81 antibody [EPR21916] (ab219209)](https://www.abcam.com/ps/products/219/ab219209/Images/ab219209-306924-anti-cd81-antibody-epr21916-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CD81 antibody [EPR21916] (ab219209)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21916] to CD81
- Suitable for: IHC-P, ICC/IF, Flow Cyt
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CD81 antibody [EPR21916]
See all CD81 primary antibodies -
Description
Rabbit monoclonal [EPR21916] to CD81 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human liver, tonsil and diffuse large B cell lymphoma tissues. ICC/IF: Jurkat cells. Flow Cyt: Jurkat cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21916 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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ELISA pair antibody
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab219209 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (1) |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| ICC/IF | (1) |
1/100.
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| Flow Cyt |
1/600.
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| Notes |
|---|
|
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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ICC/IF
1/100. |
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Flow Cyt
1/600. |
Target
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Function
May play an important role in the regulation of lymphoma cell growth. Interacts with a 16-kDa Leu-13 protein to form a complex possibly involved in signal transduction. May acts a the viral receptor for HCV. -
Tissue specificity
Hematolymphoid, neuroectodermal and mesenchymal tumor cell lines. -
Involvement in disease
Defects in CD81 are the cause of immunodeficiency common variable type 6 (CVID6) [MIM:613496]; also called antibody deficiency due to CD81 defect. CVID6 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low. -
Sequence similarities
Belongs to the tetraspanin (TM4SF) family. -
Post-translational
modificationsNot glycosylated. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 975 Human
- Omim: 186845 Human
- SwissProt: P60033 Human
- Unigene: 54457 Human
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Alternative names
- 26 kDa cell surface protein TAPA 1 antibody
- 26 kDa cell surface protein TAPA-1 antibody
- 26 kDa cell surface protein TAPA1 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-CD81 antibody [EPR21916] (ab219209)
Immunofluorescent analysis of 100% methanol-fixed Jurkat (human T cell leukemia cell line from peripheral blood) cells and HepG2 (human liver hepatocellular carcinoma cell line) cells labeling CD81 with ab219209 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing membranous staining in Jurkat cells.
Negative control: HepG2 (PMID: 16641285).
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
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![Flow Cytometry - Anti-CD81 antibody [EPR21916] (ab219209)](https://www.abcam.com/ps/products/219/ab219209/Images/ab219209-306923-anti-cd81-antibody-epr21916-flow-cytometry.jpg)
Flow Cytometry - Anti-CD81 antibody [EPR21916] (ab219209)Flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cells and HepG2 (human liver hepatocellular carcinoma cell line) cell lines labeling CD81 with ab219209 at 1/600 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells incubated with secondary antibody only) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.
Gated on viable cells.
Negative control: HepG2 (PMID: 16641285).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD81 antibody [EPR21916] (ab219209)](https://www.abcam.com/ps/products/219/ab219209/Images/ab219209-306922-anti-cd81-antibody-epr21916-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD81 antibody [EPR21916] (ab219209)Immunohistochemical analysis of paraffin-embedded human liver tissue labeling CD81 with ab219209 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Mainly membranous staining in human liver (PMID: 19489072). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD81 antibody [EPR21916] (ab219209)](https://www.abcam.com/ps/products/219/ab219209/Images/ab219209-306921-anti-cd81-antibody-epr21916-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD81 antibody [EPR21916] (ab219209)Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD81 with ab219209 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining in the germinal center of human tonsil (PMID: 20004001). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD81 antibody [EPR21916] (ab219209)](https://www.abcam.com/ps/products/219/ab219209/Images/ab219209-306920-anti-cd81-antibody-epr21916-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD81 antibody [EPR21916] (ab219209)Immunohistochemical analysis of paraffin-embedded human diffuse large B cell lymphoma tissue labeling CD81 with ab219209 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on tumor cells of human diffuse large B cell lymphoma (PMID: 20004001). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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![Anti-CD81 antibody [EPR21916] (ab219209)](https://www.abcam.com/ps/products/219/ab219209/Images/ab219209-6-benefits-of-recombinant-antibodies.png)
Anti-CD81 antibody [EPR21916] (ab219209)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (9)
ab219209 has been referenced in 9 publications.
- Governa V et al. Landscape of surfaceome and endocytome in human glioma is divergent and depends on cellular spatial organization. Proc Natl Acad Sci U S A 119:N/A (2022). PubMed: 35217608
- Guo R et al. Mesenchymal Stromal Cells-Derived Extracellular Vesicles Regulate Dendritic Cell Functions in Dry Eye Disease. Cells 12:N/A (2022). PubMed: 36611828
- Wang J et al. Exosomal transfer of miR-25-3p promotes the proliferation and temozolomide resistance of glioblastoma cells by targeting FBXW7. Int J Oncol 59:N/A (2021). PubMed: 34278448
- Si J et al. Heparanase confers temozolomide resistance by regulation of exosome secretion and circular RNA composition in glioma. Cancer Sci 112:3491-3506 (2021). PubMed: 34036683
- Li J et al. Exosomal transfer of miR-106a-5p contributes to cisplatin resistance and tumorigenesis in nasopharyngeal carcinoma. J Cell Mol Med 25:9183-9198 (2021). PubMed: 34469038
- Wang ZG et al. Comprehensive proteomic analysis of exosomes derived from human bone marrow, adipose tissue, and umbilical cord mesenchymal stem cells. Stem Cell Res Ther 11:511 (2020). PubMed: 33246507
- Dai E et al. Autophagy-dependent ferroptosis drives tumor-associated macrophage polarization via release and uptake of oncogenic KRAS protein. Autophagy 16:2069-2083 (2020). PubMed: 31920150
- Xiong Y et al. Circulating Exosomal miR-20b-5p Inhibition Restores Wnt9b Signaling and Reverses Diabetes-Associated Impaired Wound Healing. Small 16:e1904044 (2020). PubMed: 31867895
- Xian P et al. Mesenchymal stem cell-derived exosomes as a nanotherapeutic agent for amelioration of inflammation-induced astrocyte alterations in mice. Theranostics 9:5956-5975 (2019). PubMed: 31534531