Anti-CD82 antibody [TS82b] - BSA and Azide free (ab59509)
![Western blot - Anti-CD82 antibody [TS82b] - BSA and Azide free (ab59509)](https://www.abcam.com/ps/products/59/ab59509/Images/ab59509-472932-AntiCD82-antibody-TS82b-wb-human.jpg "Western blot - Anti-CD82 antibody [TS82b] - BSA and Azide free (ab59509)")
Key features and details
- Mouse monoclonal [TS82b] to CD82 - BSA and Azide free
- Suitable for: IP, ICC/IF, IHC-P, WB, Flow Cyt
- Reacts with: Rat, Human
- Isotype: IgG1
Overview
-
Product name
Anti-CD82 antibody [TS82b] - BSA and Azide free
See all CD82 primary antibodies -
Description
Mouse monoclonal [TS82b] to CD82 - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: IP, ICC/IF, IHC-P, WB, Flow Cytmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Tissue, cells or virus corresponding to Human CD82. Jurkat and HEL cell lines
-
Positive control
- Raji and HEL cells
-
General notes
This product was changed from ascites to tissue culture supernatant on 19th June 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at 4°C (stable for up to 12 months). Do Not Freeze. -
Storage buffer
pH: 7.30
Constituent: 100% PBS
Sterile-filtered through 0.22 µm. Carrier and preservative free -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
TS82b -
Myeloma
P3-x63-Ag8 -
Isotype
IgG1 -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab59509 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
Use at an assay dependent concentration.
|
|
| ICC/IF | (3) |
Use at an assay dependent concentration.
|
| IHC-P | (1) |
Use at an assay dependent concentration.
|
| WB | (1) |
Use at an assay dependent concentration. Use under non reducing condition. Predicted molecular weight: 37 kDa.
|
| Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
IP
Use at an assay dependent concentration. |
|
ICC/IF
Use at an assay dependent concentration. |
|
IHC-P
Use at an assay dependent concentration. |
|
WB
Use at an assay dependent concentration. Use under non reducing condition. Predicted molecular weight: 37 kDa. |
|
Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
-
Function
Associates with CD4 or CD8 and delivers costimulatory signals for the TCR/CD3 pathway. -
Tissue specificity
Lymphoid specific. -
Sequence similarities
Belongs to the tetraspanin (TM4SF) family. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 3732 Human
- Entrez Gene: 83628 Rat
- Omim: 600623 Human
- SwissProt: P27701 Human
- SwissProt: O70352 Rat
- Unigene: 527778 Human
- Unigene: 3022 Rat
-
Alternative names
- 4F9 antibody
- Antigen detected by monoclonal and antibody IA4 antibody
- C33 antibody
see all
Images
-
Western blot - Anti-CD82 antibody [TS82b] - BSA and Azide free (ab59509)All lanes : Anti-CD82 antibody [TS82b] - BSA and Azide free (ab59509) at 1 µg/ml
Lane 1 : Raji (Human Burkitt's lymphoma cell line) whole cell lysate at 25 µg
Lane 2 : Raji whole cell lysate at 50 µg
Lane 3 : HEL (Human bone marrow erythroleukemia cell line) whole cell lysate at 25 µg
Lane 4 : HEL whole cell lysate at 50 µg
Lane 5 : YT whole cell lysate at 25 µg
Lane 6 : YT whole cell lysate at 50 µg
Secondary
All lanes : Anti IgG1-HRP
Predicted band size: 37 kDaLysis buffer:
• Tris pH7.4, NaCl 150 mM, CaCl2 2H2O 1 mM, MgCl2 1 mM NaN3 0.02%, Triton X100 1%
Gel:
5-15% SDS polyacrylamide. -
![Flow Cytometry - Anti-CD82 antibody [TS82b] (ab59509)](https://www.abcam.com/ps/products/59/ab59509/Images/CD82-Primary-antibodies-ab59509-7.jpg)
Flow Cytometry - Anti-CD82 antibody [TS82b] (ab59509)Overlay histogram showing HeLa cells stained with ab59509 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59509, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG, H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.This image was generated using the ascites version of the product.
Datasheets and documents
-
SDS download
-
Datasheet download
References (8)
ab59509 has been referenced in 8 publications.
- Zhao Y et al. CD82-TRPM7-Numb signaling mediates age-related cognitive impairment. Geroscience 42:595-611 (2020). PubMed: 32088828
- Huang X et al. Gangliosides and CD82 inhibit the motility of colon cancer by downregulating the phosphorylation of EGFR at different tyrosine sites and signaling pathways. Mol Med Rep 22:3994-4002 (2020). PubMed: 33000220
- Du H et al. miR-601 inhibits proliferation, migration and invasion of prostate cancer stem cells by targeting KRT5 to inactivate the Wnt signaling pathway. Int J Clin Exp Pathol 12:4361-4379 (2019). PubMed: 31933840
- Brzozowski JS et al. Extracellular vesicles with altered tetraspanin CD9 and CD151 levels confer increased prostate cell motility and invasion. Sci Rep 8:8822 (2018). PubMed: 29891991
- Ni YH et al. Distinct expression patterns of Toll-like receptor 7 in tumour cells and fibroblast-like cells in oral squamous cell carcinoma. Histopathology 67:730-9 (2015). IHC . PubMed: 25828894
- Spring FA et al. Tetraspanins CD81 and CD82 facilitate a4ß1-mediated adhesion of human erythroblasts to vascular cell adhesion molecule-1. PLoS One 8:e62654 (2013). IP ; Human . PubMed: 23704882
- Saleh SM et al. Identification of the tetraspanin CD82 as a new barrier to xenotransplantation. J Immunol 191:2796-805 (2013). PubMed: 23872050
- Nagao K & Oka K HIF-2 directly activates CD82 gene expression in endothelial cells. Biochem Biophys Res Commun 407:260-5 (2011). WB ; Human . PubMed: 21382346