Recombinant Anti-CD83 antibody [EPR23809-19] (ab275021)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD83 antibody [EPR23809-19] (ab275021)](https://www.abcam.com/ps/products/275/ab275021/Images/ab275021-1-anti-cd83-antibody-epr2380919-immunohistochemistry-human-tonsil.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD83 antibody [EPR23809-19] (ab275021)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23809-19] to CD83
- Suitable for: Flow Cyt, IHC-P, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CD83 antibody [EPR23809-19]
See all CD83 primary antibodies -
Description
Rabbit monoclonal [EPR23809-19] to CD83 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, IHC-P, IPmore details
Unsuitable for: ICC/IF or WB -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human tonsil and Hodgkin's lymphoma tissue. Flow Cyt: HDLM-2 cells. IP: HDLM-2 whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23809-19 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab275021 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
1/500.
|
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| IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| IP |
1/30.
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| Notes |
|---|
|
Flow Cyt
1/500. |
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IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
IP
1/30. |
Target
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Function
May play a significant role in antigen presentation or the cellular interactions that follow lymphocyte activation. -
Tissue specificity
Expressed by activated lymphocytes, Langerhans cells and interdigitating reticulum cells. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 9308 Human
- Omim: 604534 Human
- SwissProt: Q01151 Human
- Unigene: 595133 Human
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Alternative names
- B cell activation 45kDa cell surface glycoprotein Ig superfamily antibody
- B cell activation protein antibody
- B-cell activation protein antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD83 antibody [EPR23809-19] (ab275021)
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD83 with ab275021 at 1/500 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human tonsil. The section was incubated with ab275021 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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![Flow Cytometry - Anti-CD83 antibody [EPR23809-19] (ab275021)](https://www.abcam.com/ps/products/275/ab275021/Images/ab275021-3-anti-cd83-antibody-epr2380919-flow-cytometry-hdlm2.jpg)
Flow Cytometry - Anti-CD83 antibody [EPR23809-19] (ab275021)Flow cytometric analysis of HDLM-2 (Human Hodgkin lymphoma) cells labelling CD83 with ab275021 at 1/500 dilution (0.1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Gated on viable cells.
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![Immunoprecipitation - Anti-CD83 antibody [EPR23809-19] (ab275021)](https://www.abcam.com/ps/products/275/ab275021/Images/ab275021-4-anti-cd83-antibody-epr2380919-immunoprecipitation-hdlm2.jpg)
Immunoprecipitation - Anti-CD83 antibody [EPR23809-19] (ab275021)CD83 was immunoprecipitated from 0.35 mg HDLM-2 (human hodgkin lymphoma) whole cell lysate with ab275021 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab275021 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: HDLM-2 whole cell lysate 10 µg.
Lane 2: ab275021 IP in HDLM-2 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab275021 in HDLM-2 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 145 seconds.
The expression profile observed is consistent with what has been described in the literature (PMID: 15320871).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD83 antibody [EPR23809-19] (ab275021)](https://www.abcam.com/ps/products/275/ab275021/Images/ab275021-2-anti-cd83-antibody-epr2380919-immunohistochemistry-human-hodgkins-lymphoma.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD83 antibody [EPR23809-19] (ab275021)Immunohistochemical analysis of paraffin-embedded human Hodgkin's lymphoma tissue labeling CD83 with ab275021 at 1/500 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human Hodgkin's lymphoma (PMID: 24633193). The section was incubated with ab275021 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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![Anti-CD83 antibody [EPR23809-19] (ab275021)](https://www.abcam.com/ps/products/275/ab275021/Images/ab275021-5-benefits-of-recombinant-antibodies.png)
Anti-CD83 antibody [EPR23809-19] (ab275021)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab275021 has not yet been referenced specifically in any publications.