Recombinant Anti-CD86 antibody [BLR030F] - BSA free (ab243887)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BLR030F] to CD86 - BSA free
- Suitable for: ICC, IHC-P, IP, WB
- Reacts with: Human
Overview
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Product name
Anti-CD86 antibody [BLR030F] - BSA free
See all CD86 primary antibodies -
Description
Rabbit monoclonal [BLR030F] to CD86 - BSA free -
Host species
Rabbit -
Tested applications
Suitable for: ICC, IHC-P, IP, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human CD86 aa 300-329 (C terminal). The exact sequence is proprietary. NP_787058.4 and Gene ID 942.
Database link: P42081 -
General notes
This product is sold under License from Bethyl Laboratories, Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.8
Preservative: 0.09% Sodium azide
Constituent: 99% Borate buffered saline -
Concentration information loading...
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Purification notes
Recombinant antibody was purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
BLR030F -
Isotype
IgG -
Research areas
Associated products
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab243887 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
1/100 - 1/500.
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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|
IP |
Use at an assay dependent concentration.
Use 6µl/0.5mg lysate. |
|
WB |
1/1000.
|
Notes |
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ICC
1/100 - 1/500. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
Use at an assay dependent concentration. Use 6µl/0.5mg lysate. |
WB
1/1000. |
Target
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Function
Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. -
Tissue specificity
Expressed by activated B-lymphocytes and monocytes. -
Sequence similarities
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsPolyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 942 Human
- Omim: 601020 Human
- SwissProt: P42081 Human
- Unigene: 171182 Human
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Alternative names
- Activation B7-2 antigen 3 antibody
- Activation B7-2 antigen antibody
- B-lymphocyte activation antigen B7-2 2 antibody
see all
Images
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CD86 was immunoprecipitated from 1.0 mg HDLM-2 (Human Hodgkin lymphoma cell line from pleural effusion) whole cell lysate with ab243887 at 6 µl per reaction. Western blot was performed on the immunoprecipitate using ab243887 at 1/1000 dilution.
Lane 1: ab243864 IP in HDLM-2whole cell lysate.
Lane 2: Control IgG IP in HDLM-2 whole cell lysate.
Detection: Chemiluminescence with an exposure time of 10 seconds.
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All lanes : Anti-CD86 antibody [BLR030F] - BSA free (ab243887) at 1/1000 dilution
Lane 1 : HDLM-2 (Human Hodgkin lymphoma cell line from pleural effusion) whole cell lysate at 5 µg
Lane 2 : MJ (Human primary cutaneous T-cell non-Hodgkin lymphoma from peripheral blood) whole cell lysate at 5 µg
Lane 3 : SUP-M2 (Human ALK-positive anaplastic large cell lymphoma from cerebrospinal fluid) whole cell lysate at 50 µg
Lane 4 : KG-1 (Human acute myeloid leukemia from bone narrow) whole cell lysate at 50 µg
Lane 5 : MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate at 50 µg
Lane 6 : Ramos (Human Burkitt's lymphoma cell line) whole cell lysate at 50 µg
Lane 7 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Lane 8 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 50 µg
Lane 9 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 10 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 50 µg
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG antibodyDetection: chemiluminescence with an exposure time of 3 minutes.
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Formalin-fixed, paraffin-embedded HDLM-2 cells labeling CD86 using ab243887 at 1/200 dilution in ICC analysis. A HRP-conjugated goat-anti rabbit IgG was used as the secondary. DAB staining.
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Formalin-fixed, paraffin-embedded human seminoma tissue stained for CD86 using ab243860 at 1/200 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
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Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD86 using ab243887 at 1/200 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab243887 has been referenced in 3 publications.
- Li X et al. Oleandrin, a cardiac glycoside, induces immunogenic cell death via the PERK/elF2a/ATF4/CHOP pathway in breast cancer. Cell Death Dis 12:314 (2021). PubMed: 33762577
- Qiu H et al. Integrated Analysis Reveals Prognostic Value and Immune Correlates of CD86 Expression in Lower Grade Glioma. Front Oncol 11:654350 (2021). PubMed: 33954112
- Yao Q et al. Bioadhesive hydrogel comprising bilirubin/ß-cyclodextrin inclusion complexes promote diabetic wound healing. Pharm Biol 59:1139-1149 (2021). PubMed: 34425063