Recombinant Anti-CD93 antibody [EPR5386] (ab134079)
![Flow Cytometry (Intracellular) - Anti-CD93 antibody [EPR5386] (ab134079)](https://www.abcam.com/ps/products/134/ab134079/Images/ab134079-148222-anti-cd93-antibody-epr5386-flow-cytometry.jpg "Flow Cytometry (Intracellular) - Anti-CD93 antibody [EPR5386] (ab134079)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5386] to CD93
- Suitable for: WB, Flow Cyt (Intra)
- Reacts with: Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-CD93 antibody [EPR5386]
See all CD93 primary antibodies -
Description
Rabbit monoclonal [EPR5386] to CD93 -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cyt (Intra)more details
Unsuitable for: ELISA,IHC-P or IP -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- THP-1, Rat kidney, and Human platelet lysates, THP-1 cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.80 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5386 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134079 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 69 kDa).
|
|
| Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 69 kDa). |
|
Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Application notes
Is unsuitable for ELISA,IHC-P or IP.
Target
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Function
Receptor (or element of a larger receptor complex) for C1q, mannose-binding lectin (MBL2) and pulmonary surfactant protein A (SPA). May mediate the enhancement of phagocytosis in monocytes and macrophages upon interaction with soluble defense collagens. May play a role in intercellular adhesion. -
Tissue specificity
Highly expressed in endothelial cells, platelets, cells of myeloid origin, such as monocytes and neutrophils. Not expressed in cells of lymphoid origin. -
Sequence similarities
Contains 1 C-type lectin domain.
Contains 5 EGF-like domains. -
Post-translational
modificationsN- and O-glycosylated. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 22918 Human
- Entrez Gene: 17064 Mouse
- Entrez Gene: 84398 Rat
- Omim: 120577 Human
- SwissProt: Q9NPY3 Human
- SwissProt: O89103 Mouse
- SwissProt: Q9ET61 Rat
- Unigene: 97199 Human
see all -
Alternative names
- C1q receptor 1 antibody
- C1q/MBL/SPA receptor antibody
- C1qR antibody
see all
Images
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Flow Cytometry (Intracellular) - Anti-CD93 antibody [EPR5386] (ab134079)
Intracellular flow cytometric analysis of permeabilized THP-1 cells labelling CD93 red with ab134079 at 1/100 and a rabbit IgG negative (green).
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![Western blot - Anti-CD93 antibody [EPR5386] (ab134079)](https://www.abcam.com/ps/products/134/ab134079/Images/ab134079-148221-anti-cd93-antibody-epr5386-western-blot.jpg)
Western blot - Anti-CD93 antibody [EPR5386] (ab134079)All lanes : Anti-CD93 antibody [EPR5386] (ab134079) at 1/1000 dilution
Lane 1 : THP-1 lysate
Lane 2 : Rat kidney lysate
Lane 3 : Human platelet lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti-rabbit at 1/2000 dilution
Predicted band size: 69 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted? -
![OI-RD Scanning - Anti-CD93 antibody [EPR5386] (ab134079)](https://www.abcam.com/ps/products/134/ab134079/Images/ab134079-148220-anti-cd93-antibody-epr5386.jpg)
OI-RD Scanning - Anti-CD93 antibody [EPR5386] (ab134079) -
![Anti-CD93 antibody [EPR5386] (ab134079)](https://www.abcam.com/ps/products/134/ab134079/Images/ab134079-4-benefits-of-recombinant-antibodies.png)
Anti-CD93 antibody [EPR5386] (ab134079)
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab134079 has been referenced in 3 publications.
- Roth M et al. Pelargonium sidoides radix extract EPs 7630 reduces rhinovirus infection through modulation of viral binding proteins on human bronchial epithelial cells. PLoS One 14:e0210702 (2019). PubMed: 30707726
- Liu W et al. Radioimmunoimaging of 125I-labeled anti-CD93 monoclonal antibodies in a xenograft model of non-small cell lung cancer. Oncol Lett 18:6413-6422 (2019). PubMed: 31819775
- Roth M et al. Broncho Vaxom (OM-85) modulates rhinovirus docking proteins on human airway epithelial cells via Erk1/2 mitogen activated protein kinase and cAMP. PLoS One 12:e0188010 (2017). PubMed: 29182620