Recombinant Anti-CD97 antibody [EPR4427] (ab108368)

J’ai contacté le laboratoire qui m’a confirmé que les IgG de lapins n’ont pas de sous-classes comme les IgG de souris et d'humains. L’isotype d’ab108368 est donc simplement IgG.

Thank you for contacting us.

I have spoken to my colleague who suggested ab108368 (https://www.abcam.com/ab108368) to you. This antibody is a rabbit monoclonal which has been tested in immunocytochemistry, immunohistochemistry (paraffin), immunoprecipitation and western blots.

Unfortunately, this antibody has also been tested in flow cytometry and has been found to be unsuitable for use in this application so we will not be able to offer you a testing discount as previously mentioned by my colleague. I am very sorry about this error on our part.

However, if you would like to use an anti-mouse CD97 in flow cytometry, I recommend using ab135182 (https://www.abcam.com/ab135182). This antibody is a rat monoclonal and has been successfully tested in flow cytometry and is therefore guaranteed for use in this application.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Thank you for your response and for your continued patronage of Abcam. I am glad that you enjoyed the brownies!

When ordering, it is most beneficial to use our website or to call our customer service representatives as they are much more knowledgeable of the ordering system and would be able to effectively resolve any issues should they arise.

Thank you again contacting me and for your kind words. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

I has been my pleasure to help with this. I would like to thank you for your help as well and for your cooperation. The details that you have provided will enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement of this product for ab122999. The order number for this replacement is 1040525.


This product is expected not in stock at the moment but is expected to ship next week. To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

Thank you for sending me this further information. It has helped tremendously in understanding the issues that you have been facing.

I agree with your assessment that these bands are either non-specific or isoforms 4 and 5. As we cannot be sure which and I can see no futher suggestions that I might make which you have not already tried. If you have purchase this antibody wuithin the last six months, it will be covered by our Abpromise guarantee. In which case I mayoffer a free of charge replacement for this product.

This issue may be specific to this vial or lot in wehich case I can offer you a new vial of ab75190. I beleive that I have already also communicated the immunogen sequence to you so should you decide to perform an immungen blocking procedure that peptide could easliy be created.

As we had discussed earlier ab122999should recognize isoforms 1, 2, 3 and 4, as there is 100% homology while there is only 76% homology to isoform 5. However, the immungen for the product is published should it be neccessary to create a blocking peptide as well.

I look forward to hearing from you, please be sure to send the Abcam order confirmation number or PO number used. I've included a pdf file overviewing immunogen blocking procedures. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.







I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

I am sorry about the issues you have been having. Thank you for bringing this to our attention. As this product should not be recognizing the other forms of EMR2 it appears that you may be experiencing non specific background issues. I may be able to help you reduce or eliminate these issues if yopu could send me the details of your protocol and any images you may have. I've put a number of questions below which will help me to get the clearest possible picture of the issue you are facing. Please forgive me if we have spoken about some of these before as my notes are rather light in regard to whether we had spoken about your protocols.

I see that you have been working with Human tissues, could you tell me the lysis buffer that you have used?

Have you reduced and denatured your samples? Which method/buffers did you use?

How much total protein did you use per well?

Did you load a loading control? Which LC did you use? What were the results?

Did you load a positive control? Which PC did you use? What were the results?

What membrane type did you transfer to?

What blocking steps did you use?

What dilutions of this product did you use? How long and at what temperature was this antibody incubated?

What was the secondary antibody that you have used? How long and at what temperature was this antibody incubated?

Have you run a no primary antibody control blot? What were the results?

What is the wash buffer that you use?

Could you provide the Abcam order confirmation number or PO number used to purchase this?

Thank you very much for taking the time to answer these questions. Please contact me if you have any questions or concerns.

I have had to opportunity to speak with the lab further regarding this. The immunogen sequence for this product is:XXX which spans aaXXX-XXX (SwissProt: Q9UHX3Human). This sequence is within the excellular region and is not in isoforms 4 and 5. Are you experiencing non-specific background bands at these weights? It may help to adjust your protocol slightly to reduce or eliminate these. Some steps that may be taken include changing your blocking solution to 5% BSA if using milk, lowering the concentration of the primary antibody is often helpful as well. The antibody is covered under our Abpromise for six months and is guaranteed to work ib WBon humansamples. If you are experiencing issues with this product and we cannot resolve the issue you are having , then I would be happy to either send a replacement antibody or to process a refund. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

Thank you for contacting us.

I have had the opportunity to speak with the labs in regard to your questions about these product.

The immunogen sequence for each product is proprietary but the labs have run alignments and checked homologies with other isoforms. The results are as follows:



ab122999: Based on the peptide immunogen used to generate our anti-EMR2 antibody (ARP61772_P050) and the UniProt EMR2 protein page (http://www.uniprot.org/uniprot/Q9UHX3), this antibody should recognize isoforms 1, 2, 3 and 4, as there is 100% homology. There is only 76% homology to isoform 5 (11 consecutive matches, 3 non-matches on the end of the 14aa immunogen), which is near the threshold of what we would predict for reactivity (80%).



ab108368: We have not tested this antibody for recognition of other isoforms. Based on sequence analysis, the immunogen shares 100% identity with isoforms 2 and 3. It is predicted, but it is not a guarantee without testing.



ab75190: Human EMR2 (CD312) has five isoforms (isoform 1-5). This antibody reacts with isoform 1, 2, and 3. It does not react with isoform 4 and 5. The reaction domain is extracellular domain (on the first topological domain)



I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.