Anti-CDA antibody (ab231981)
Key features and details
- Rabbit polyclonal to CDA
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-CDA antibody
See all CDA primary antibodies -
Description
Rabbit polyclonal to CDA -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human, Pig -
Immunogen
Recombinant full length protein (His-T7-tag) corresponding to Human CDA aa 1 to the C-terminus. Expresssed in E.coli.
Database link: P32320 -
Positive control
- WB: Recombinant human CDA protein; HeLa whole cell lysate; Pig kidney tissue lysate. IHC-P: Human kidney, lung cancer, skin cancer and bile duct cancer tissue. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab231981 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 5 - 20 µg/ml.
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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WB |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 16 kDa.
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Notes |
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ICC/IF
Use a concentration of 5 - 20 µg/ml. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 16 kDa. |
Target
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Relevance
CDA (Cytidine deaminase) scavengers exogenous and endogenous cytidine and 2'-deoxycytidine for UMP synthesis. Growth inhibition of granulocyte-macrophage colony foring cells by human cytidine deaminase requires the catalytic function of the protein. It is highly expressed in granulocytes. -
Database links
- Entrez Gene: 978 Human
- Omim: 123920 Human
- SwissProt: P32320 Human
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Alternative names
- CDD antibody
- Cytidine aminohydrolase antibody
- Cytidine deaminase antibody
- EC 3.5.4.5 antibody
Images
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Formalin-fixed, paraffin-embedded human kidney tissue stained for CDA using ab231981 at 10 μg/ml in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human lung cancer tissue stained for CDA using ab231981 at 20 μg/ml in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human skin cancer tissue stained for CDA using ab231981 at 20 μg/ml in immunohistochemical analysis.
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HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for CDA using ab231981 at 20μg/ml in ICC/IF.
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Formalin-fixed, paraffin-embedded human bile duct cancer tissue stained for CDA using ab231981 at 20 μg/ml in immunohistochemical analysis.
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Anti-CDA antibody (ab231981) at 3 µg/ml + Recombinant human CDA
Secondary
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 16 kDa -
All lanes : Anti-CDA antibody (ab231981) at 3 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : Pig kidney lysate
Secondary
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 16 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab231981 has not yet been referenced specifically in any publications.