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Anti-CDA antibody (ab56053)

Submit a review Q&A (3)References (12)

Key features and details

  • Rabbit polyclonal to CDA
  • Suitable for: ELISA, WB
  • Reacts with: Recombinant fragment
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-CDA antibody [EPR20525] (ab222515)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-CDA antibody
    See all CDA primary antibodies

  • Description

    Rabbit polyclonal to CDA

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ELISA, WBmore details

  • Species reactivity

    Reacts with: Recombinant fragment
    Predicted to work with: Human

  • Immunogen

    Synthetic peptide corresponding to N terminal residues of Human CDA.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab56053 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 16 kDa.

This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
Notes
ELISA
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 16 kDa.

This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.

Target

  • Relevance

    CDA (Cytidine deaminase) scavengers exogenous and endogenous cytidine and 2'-deoxycytidine for UMP synthesis. Growth inhibition of granulocyte-macrophage colony foring cells by human cytidine deaminase requires the catalytic function of the protein. It is highly expressed in granulocytes.

  • Database links

    • Alternative names

      • CDD antibody
      • Cytidine aminohydrolase antibody
      • Cytidine deaminase antibody
      • EC 3.5.4.5 antibody

    References (12)

    Publishing research using ab56053? Please let us know so that we can cite the reference in this datasheet.

    ab56053 has been referenced in 12 publications.

    • Mameri H  et al. Cytidine deaminase deficiency in tumor cells is associated with sensitivity to a naphthol derivative and a decrease in oncometabolite levels. Cell Mol Life Sci 79:465 (2022). PubMed: 35925417
    • Silveira SC  et al. A decrease in NAMPT activity impairs basal PARP-1 activity in cytidine deaminase deficient-cells, independently of NAD. Sci Rep 10:13907 (2020). PubMed: 32807821
    • Furusho K  et al. Cytidine deaminase enables Toll-like receptor 8 activation by cytidine or its analogs. Int Immunol N/A:N/A (2018). PubMed: 30535046
    • Bou Samra E  et al. A role for Tau protein in maintaining ribosomal DNA stability and cytidine deaminase-deficient cell survival. Nat Commun 8:693 (2017). PubMed: 28947735
    • Gemble S  et al. Cytidine deaminase deficiency impairs sister chromatid disjunction by decreasing PARP-1 activity. Cell Cycle 16:1128-1135 (2017). PubMed: 28463527
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Question

    o echnique does not require any optimization and it works very well with all the other antibodies we have, (including abcam) we tried some of your suggestions but without any successful result.
    Increasing dilution of the secondary are not decreasing the unspecifity but only increasing the time of exposure to get visible bands.
    Our secondary antibody does not create any unspecific binding with any other antibody it is Affinity purified goat anti-rabbit IgG (H&L) antibody conjugated to horseradish peroxidase.
    At this point we have already wasted too much time and a lot of other reagents to run blots with different conditions which have shown unsuccessful results to the antibody.
    We would like to receive these two substitutions as soon as possible.
    Cda ab82346
    Dck ab96599

    Read More
    Answer

    rdetails and for your cooperation.
    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused.
    I appreciate the time you have spent on these experiments in the laboratory and it is disappointing the results have not been successful so far. However, we often find that suggesting some scientifically thought out optimisation tips improves the results. This is obviously a preferable outcome for the customer. So I hope you can understand that our policy is to offer the best technical support possible to help optimise the results first.
    The details provided in the questionnaire enable us to closely monitor the quality of our products.
    As requested, I have issued a free of charge replacement
    To check the status of the order please contact our Customer Service team and reference this number.
    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.
    I wish you the best of luck with your research.

    Read More

    Question

    Following our telephone conversation regarding the cda and dck antibody that we have recently ordered from abcam, I am attaching both the questionnaire and a PowerPoint slide containing the result of the blots.

    In substitution of the antibodies that we have ordered we would like to try those two other clones:

    Cda ab82346

    Dck ab96599




    Abcam product code ab




    Anti CDA antibody Ab56053

    Anti DCK antibody Ab38012




    Abcam order reference number or product batch number



    Description of the problem




    I get a lot of background of unspecific bands that unable to let me predict which is the band of interest



    4) Sample preparation:

    Type of sample: cell lysates

    Lysis buffer : CELL LYTIC M CELL LYSIS REAGENT

    Protease inhibitors: PROTEASE INHIBITOR COCKTAIL SIGMA

    Phosphatase inhibitors : PHOSPHATASE INHIBITOR ACTIVE MOTIF

    Reducing agent N/A

    Boiling for ≥5 min? yes

    Protein loaded ug/lane or cells/lane : 30ug per well

    Positive control : Calnexin

    Negative control N/A



    5) Percentage of gel: 7% or 4-12% precast gel

    Type of membrane IMMOBILON V TRANSFER MEMBRANE

    Protein transfer verified yes

    Blocking agent and concentration 5%BSA in TBS-T

    Blocking time 1h

    Blocking temperature room temperature



    6) Primary antibody (If more than one was used, describe in “additional notes”) :

    Concentration or dilution

    1:1000

    Diluent buffer 5%BSA IN TBS-T

    Incubation time OVERNIGHT

    Incubation temperature: AT 4 DEGREES



    7) Secondary antibody:

    Species: ANTIRABBIT

    Reacts against: HUMAN

    Concentration or dilution 1:1000

    Diluent buffer 5% BSA IN TBS-T

    Incubation time 1H

    Incubation temperature: ROOM TEMPERATURE

    Fluorochrome or enzyme conjugate: ECL



    8) Washing after primary and secondary antibodies:

    Buffer (TBST-TWEEN)

    Number of washes 3 WASHES



    9)Detection method ECL

    10) How many times have you run this staining? AT LEAST 3

    Do you obtain the same results every time? YES

    What steps have you altered to try and optimize the use of this antibody?

    I TRIED TO BLOCK IN MILK

    I USED 2 DIFFERENT DILUTIONS FOR DCK AND CDA: 1: 1000 and 1:500

    Read More
    Answer

    Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results.
    The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality.
    Having reviewed this case, I would like to offer some suggestions to help optimize the results from ab38012 and ab56053. I would also appreciate if you can confirm some further details:
    Overall, your protocol looks all right. However, the secondary concentration is unusual high. Have you considered a non-primary control? In addition, I would advise to try the antibody in a much lower dilution, at least 1:10000.
    Another point is the specificity of the secondary antibody, which is a bit confusing: Does this antibody react against human?

    Should the suggestions not improve the results, please do let me know.
    In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund.

    Read More

    Question

    Both antibodies do not work in the given dilution range or even in a higher. BSA as blocking agent

    Read More
    Answer

    Thank you for calling us and for alerting us to the problem you are experiencing with our product. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.
    As we discussed on the phone, I am our questionnaire so that we can gather further information regarding the samples tested and the protocol used. Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.
    I look forward to receiving your reply.

    Read More

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