Anti-CDA antibody (ab78231)
Key features and details
- Rabbit polyclonal to CDA
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-CDA antibody
See all CDA primary antibodies -
Description
Rabbit polyclonal to CDA -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to N-terminal residues of human CDA
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Positive control
- Paraffin embedded human spleen tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab78231 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 16 kDa.
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| IHC-P |
Use a concentration of 5 µg/ml.
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| Notes |
|---|
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 16 kDa. |
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IHC-P
Use a concentration of 5 µg/ml. |
Target
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Relevance
CDA (Cytidine deaminase) scavengers exogenous and endogenous cytidine and 2'-deoxycytidine for UMP synthesis. Growth inhibition of granulocyte-macrophage colony foring cells by human cytidine deaminase requires the catalytic function of the protein. It is highly expressed in granulocytes. -
Database links
- Entrez Gene: 978 Human
- Omim: 123920 Human
- SwissProt: P32320 Human
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Alternative names
- CDD antibody
- Cytidine aminohydrolase antibody
- Cytidine deaminase antibody
- EC 3.5.4.5 antibody
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDA antibody (ab78231)Immunohistochemical analysis of CDA expression in Formalin-Fixed Paraffin Embedded human spleen using 5 µg/ml ab78231
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Western blot - Anti-CDA antibody (ab78231)Anti-CDA antibody (ab78231) at 1 µg/ml + Human spleen tissue lysate - total protein (ab29699) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 16 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutes
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab78231 has been referenced in 1 publication.
- Murakami Y et al. The augmented expression of the cytidine deaminase gene by 5-azacytidine predicts therapeutic efficacy in myelodysplastic syndromes. Oncotarget 10:2270-2281 (2019). PubMed: 31040918