RecombinantRabMAb

Recombinant Anti-CDA antibody [EPR20525] (ab222515)

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Western blot - Anti-CDA antibody [EPR20525] (ab222515)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDA antibody [EPR20525] (ab222515)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDA antibody [EPR20525] (ab222515)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDA antibody [EPR20525] (ab222515)
  • Flow Cytometry (Intracellular) - Anti-CDA antibody [EPR20525] (ab222515)
  • Anti-CDA antibody [EPR20525] (ab222515)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20525] to CDA
  • Suitable for: Flow Cyt (Intra), WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-CDA antibody [EPR20525]
    See all CDA primary antibodies

  • Description

    Rabbit monoclonal [EPR20525] to CDA

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, HepG2 and TF-1 whole cell lysates; Human fetal spleen and fetal kidney lysates. IHC-P: Human spleen, liver, colon, liver cancer and colon cancer tissues. Flow Cyt (intra): HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR20525

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab222515 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/70.
WB
1/1000. Predicted molecular weight: 16 kDa.
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
Flow Cyt (Intra)
1/70.
WB
1/1000. Predicted molecular weight: 16 kDa.
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Relevance

    CDA (Cytidine deaminase) scavengers exogenous and endogenous cytidine and 2'-deoxycytidine for UMP synthesis. Growth inhibition of granulocyte-macrophage colony foring cells by human cytidine deaminase requires the catalytic function of the protein. It is highly expressed in granulocytes.

  • Database links

    • Alternative names

      • CDD antibody
      • Cytidine aminohydrolase antibody
      • Cytidine deaminase antibody
      • EC 3.5.4.5 antibody

    Images

    • Western blot - Anti-CDA antibody [EPR20525] (ab222515)
      Western blot - Anti-CDA antibody [EPR20525] (ab222515)
      All lanes : Anti-CDA antibody [EPR20525] (ab222515) at 1/1000 dilution

      Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 2 : Human fetal spleen lysate
      Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
      Lane 4 : TF-1 (human bone marrow erythroleukemia cell line) whole cell lysate
      Lane 5 : Human fetal kidney lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Developed using the ECL technique.

      Predicted band size: 16 kDa
      Observed band size: 16 kDa



      Exposure times: Lane 1: 3 minutes; Lane 2: 8 seconds; Lanes 3/5: 5 seconds; Lane 4: 30 seconds.

      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDA antibody [EPR20525] (ab222515)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDA antibody [EPR20525] (ab222515)

      Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling CDA with ab222515 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear and cytoplasmic staining on neutrophils of human spleen (PMID: 11069255). Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDA antibody [EPR20525] (ab222515)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDA antibody [EPR20525] (ab222515)

      Immunohistochemical analysis of paraffin-embedded human normal liver (panel A) and liver cancer (panel B) tissues labeling CDA with ab222515 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic and nuclear staining on normal human liver tissues, with only sporadic stromal cells showing positive staining in human liver cancer. The IHC signal on human liver cancer tissue was much lower than its corresponding normal tissue (PMID: 9849491). Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDA antibody [EPR20525] (ab222515)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDA antibody [EPR20525] (ab222515)

      Immunohistochemical analysis of paraffin-embedded human normal colon (panel A) and colon cancer (panel B) tissues labeling CDA with ab222515 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic and nuclear staining on sporadic stromal cells of human normal colon tissue, while adjacent cancer cells and some stromal cells show moderate positive staining. The IHC signal on human colon cancer tissue was higher than its corresponding normal tissue (PMID: 9849491). Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Flow Cytometry (Intracellular) - Anti-CDA antibody [EPR20525] (ab222515)
      Flow Cytometry (Intracellular) - Anti-CDA antibody [EPR20525] (ab222515)

      Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HeLa (humanepithelialcell line from cervix adenocarcinoma) cell line labeling CDA with ab222515 at 1/70 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody. 

       

       

    • Anti-CDA antibody [EPR20525] (ab222515)
      Anti-CDA antibody [EPR20525] (ab222515)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (1)

    Publishing research using ab222515? Please let us know so that we can cite the reference in this datasheet.

    ab222515 has been referenced in 1 publication.

    • Hu C  et al. circFARP1 enables cancer-associated fibroblasts to promote gemcitabine resistance in pancreatic cancer via the LIF/STAT3 axis. Mol Cancer 21:24 (2022). PubMed: 35045883

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