RecombinantRabMAb

Recombinant Anti-CDA antibody [EPR20525] - BSA and Azide free (ab227815)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDA antibody [EPR20525] - BSA and Azide free (ab227815)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDA antibody [EPR20525] - BSA and Azide free (ab227815)
  • Flow Cytometry (Intracellular) - Anti-CDA antibody [EPR20525] - BSA and Azide free (ab227815)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDA antibody [EPR20525] - BSA and Azide free (ab227815)
  • Anti-CDA antibody [EPR20525] - BSA and Azide free (ab227815)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20525] to CDA - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

Overview

  • Product name

    Anti-CDA antibody [EPR20525] - BSA and Azide free
    See all CDA primary antibodies

  • Description

    Rabbit monoclonal [EPR20525] to CDA - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human spleen tissue.

  • General notes

    ab227815 is the carrier-free version of ab222515.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab227815 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 16 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 16 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Relevance

    CDA (Cytidine deaminase) scavengers exogenous and endogenous cytidine and 2'-deoxycytidine for UMP synthesis. Growth inhibition of granulocyte-macrophage colony foring cells by human cytidine deaminase requires the catalytic function of the protein. It is highly expressed in granulocytes.

  • Database links

    • Alternative names

      • CDD antibody
      • Cytidine aminohydrolase antibody
      • Cytidine deaminase antibody
      • EC 3.5.4.5 antibody

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDA antibody [EPR20525] - BSA and Azide free (ab227815)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDA antibody [EPR20525] - BSA and Azide free (ab227815)

      Immunohistochemical analysis of paraffin-embedded human normal liver (panel A) and liver cancer (panel B) tissues labeling CDA with ab222515 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic and nuclear staining on normal human liver tissues, with only sporadic stromal cells showing positive staining in human liver cancer. The IHC signal on human liver cancer tissue was much lower than its corresponding normal tissue (PMID: 9849491). Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab222515).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDA antibody [EPR20525] - BSA and Azide free (ab227815)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDA antibody [EPR20525] - BSA and Azide free (ab227815)

      Immunohistochemical analysis of paraffin-embedded human normal colon (panel A) and colon cancer (panel B) tissues labeling CDA with ab222515 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic and nuclear staining on sporadic stromal cells of human normal colon tissue, while adjacent cancer cells and some stromal cells show moderate positive staining. The IHC signal on human colon cancer tissue was higher than its corresponding normal tissue (PMID: 9849491). Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab222515).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Flow Cytometry (Intracellular) - Anti-CDA antibody [EPR20525] - BSA and Azide free (ab227815)
      Flow Cytometry (Intracellular) - Anti-CDA antibody [EPR20525] - BSA and Azide free (ab227815)

      Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling  CDA with ab222515 at 1/70 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody. 

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab222515). 

       

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDA antibody [EPR20525] - BSA and Azide free (ab227815)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDA antibody [EPR20525] - BSA and Azide free (ab227815)

      Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling CDA with ab222515 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear and cytoplasmic staining on neutrophils of human spleen (PMID: 11069255). Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab222515).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Anti-CDA antibody [EPR20525] - BSA and Azide free (ab227815)
      Anti-CDA antibody [EPR20525] - BSA and Azide free (ab227815)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab227815? Please let us know so that we can cite the reference in this datasheet.

    ab227815 has not yet been referenced specifically in any publications.

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