Recombinant Anti-Cdc25B antibody [EPR3459(2)] (ab124819)
![Western blot - Anti-Cdc25B antibody [EPR3459(2)] (ab124819)](https://www.abcam.com/ps/products/124/ab124819/Images/ab124819-2-anti-cdc25b-antibody-epr3459-2-western-blot-nih3t3-embryo-mouse.jpg "Western blot - Anti-Cdc25B antibody [EPR3459(2)] (ab124819)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3459(2)] to Cdc25B
- Suitable for: WB, IP
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-Cdc25B antibody [EPR3459(2)]
See all Cdc25B primary antibodies -
Description
Rabbit monoclonal [EPR3459(2)] to Cdc25B -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human Cdc25B aa 150-250. The exact sequence is proprietary.
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Positive control
- K562 cell lysate, U937 cell lysate and THP-1 cell lysate, mouse embryo E17 and NIH3T3 cell lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3459(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124819 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).
|
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| IP |
1/10 - 1/100.
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| Notes |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa). |
|
IP
1/10 - 1/100. |
Application notes
Is unsuitable for Flow Cyt.
Target
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Function
Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity. -
Sequence similarities
Belongs to the MPI phosphatase family.
Contains 1 rhodanese domain. -
Post-translational
modificationsPhosphorylated by BRSK1 in vitro. Phosphorylated by CHEK1, which inhibits the activity of this protein. Phosphorylation at Ser-353 by AURKA might locally participate in the control of the onset of mitosis. Phosphorylation by MELK at Ser-169 promotes localization to the centrosome and the spindle poles during mitosis. Phosphorylation at Ser-323 and Ser-375 by MAPK14 is required for binding to 14-3-3 proteins. -
Cellular localization
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. - Information by UniProt
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Database links
- Entrez Gene: 994 Human
- Entrez Gene: 12531 Mouse
- Omim: 116949 Human
- SwissProt: P30305 Human
- SwissProt: P30306 Mouse
- Unigene: 153752 Human
- Unigene: 38444 Mouse
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Alternative names
- AI604853 antibody
- Cdc 25B antibody
- Cdc25b antibody
see all
Images
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Western blot - Anti-Cdc25B antibody [EPR3459(2)] (ab124819)All lanes : Anti-Cdc25B antibody [EPR3459(2)] (ab124819) at 0.486 µg/ml
Lane 1 : NIH/3T3 (Mouse embryonic fibroblast) cell lysates
Lane 2 : Mouse embryo E17 lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml
Predicted band size: 64 kDa
Observed band size: 64 kDa
Blocking/diluting Buffer and concentration: 5% NFDM /TBST -
![Western blot - Anti-Cdc25B antibody [EPR3459(2)] (ab124819)](https://www.abcam.com/ps/products/124/ab124819/Images/ab124819-138739-anti-cdc25b-antibody-epr34592-western-blot.jpg)
Western blot - Anti-Cdc25B antibody [EPR3459(2)] (ab124819)All lanes : Anti-Cdc25B antibody [EPR3459(2)] (ab124819) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : U937 cell lysate
Lane 3 : THP-1 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution
Predicted band size: 64 kDa
Observed band size: 64 kDa -
![Anti-Cdc25B antibody [EPR3459(2)] (ab124819)](https://www.abcam.com/ps/products/124/ab124819/Images/ab124819-3-benefits-of-recombinant-antibodies.png)
Anti-Cdc25B antibody [EPR3459(2)] (ab124819)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab124819 has been referenced in 5 publications.
- Du Y et al. SOCS7/HuR/FOXM1 signaling axis inhibited high-grade serous ovarian carcinoma progression. J Exp Clin Cancer Res 41:185 (2022). PubMed: 35624501
- Zhao J et al. lncRNA LIFR-AS1 inhibits gastric carcinoma cell proliferation, migration and invasion by sponging miR-4698. Mol Med Rep 23:N/A (2021). PubMed: 33355363
- Ding L et al. Cdc25B is transcriptionally inhibited by IER5 through the NF-YB transcription factor in irradiation-treated HeLa cells. Toxicol Res (Camb) 10:875-884 (2021). PubMed: 34484679
- Wang K et al. Corosolic acid induces cell cycle arrest and cell apoptosis in human retinoblastoma Y-79 cells via disruption of MELK-FoxM1 signaling. Oncol Rep 39:2777-2786 (2018). PubMed: 29620242
- Zhong Y et al. KCTD12 promotes tumorigenesis by facilitating CDC25B/CDK1/Aurora A-dependent G2/M transition. Oncogene 36:6177-6189 (2017). PubMed: 28869606