Recombinant Anti-CDC42 antibody [EPR15620] (ab187643)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15620] to CDC42
- Suitable for: IHC-P, IP, WB, Flow Cyt (Intra), ICC/IF
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-CDC42 antibody [EPR15620]
See all CDC42 primary antibodies -
Description
Rabbit monoclonal [EPR15620] to CDC42 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, IP, WB, Flow Cyt (Intra), ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK-293T, HepG2, MCF7, HT-29, Jurkat and HeLa whole cell lysate (ab150035); Mouse brain, Mouse kidney, Mouse spleen and Rat spleen lysates. IP: HT-29 cell lysate. Flow Cyt (intra): HeLa cells. ICC/IF: U937 cells. IHC-P: Human lung carcinoma, breast carcinoma and colon tissues; Rat colon tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15620 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Alexa Fluor® 488 Anti-CDC42 antibody [EPR15620] (ab214952)
- Alexa Fluor® 647 Anti-CDC42 antibody [EPR15620] (ab215234)
- HRP Anti-CDC42 antibody [EPR15620] (ab215810)
- Anti-CDC42 antibody [EPR15620] - Low endotoxin, Azide free (ab221781)
- APC Anti-CDC42 antibody [EPR15620] (ab225345)
- PE Anti-CDC42 antibody [EPR15620] (ab225346)
- Anti-CDC42 antibody [EPR15620] - BSA and Azide free (ab271953)
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Compatible Secondaries
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Conjugation kits
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
- HeLa whole cell lysate (ab150035)
- Mouse kidney normal tissue lysate - total protein (ab29305)
- HeLa whole cell lysate (ab29545)
- Jurkat whole cell lysate (ab30128)
- Mouse brain tissue lysate - total protein (ab30151)
- HT-29 whole cell lysate (ab3952)
- Jurkat whole cell lysate (ab7899)
- Mouse spleen tissue lysate - total protein (ab7937)
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab187643 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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IP |
1/20 - 1/40.
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WB |
1/10000 - 1/50000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
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Flow Cyt (Intra) |
1/170.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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ICC/IF | (1) |
1/500.
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Notes |
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IHC-P
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IP
1/20 - 1/40. |
WB
1/10000 - 1/50000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa). |
Flow Cyt (Intra)
1/170. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ICC/IF
1/500. |
Target
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Function
Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Causes the formation of thin, actin-rich surface projections called filopodia. -
Sequence similarities
Belongs to the small GTPase superfamily. Rho family. CDC42 subfamily. -
Post-translational
modificationsAMPylation at Tyr-32 and Thr-35 are mediated by bacterial enzymes in case of infection by H.somnus and V.parahaemolyticus, respectively. AMPylation occurs in the effector region and leads to inactivation of the GTPase activity by preventing the interaction with downstream effectors, thereby inhibiting actin assembly in infected cells. It is unclear whether some human enzyme mediates AMPylation; FICD has such ability in vitro but additional experiments remain to be done to confirm results in vivo. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 998 Human
- Entrez Gene: 12540 Mouse
- Entrez Gene: 64465 Rat
- Omim: 116952 Human
- SwissProt: P60953 Human
- SwissProt: P60766 Mouse
- SwissProt: Q8CFN2 Rat
- Unigene: 467637 Human
see all -
Form
There are 2 isoforms produced by alternative splicing. Isoform 1 also known as: Brain; Isoform 2 also known as: Placental. -
Alternative names
- CDC42 antibody
- CDC42_HUMAN antibody
- CDC42Hs antibody
see all
Images
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All lanes : Anti-CDC42 antibody [EPR15620] (ab187643) at 1/5000 dilution
Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : CDC42 knockout HEK-293T cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 21 kDaLanes 1-4: Merged signal (red and green). Green - ab187643 observed at 20 kDa. Red - loading control ab8245 observed at 37 kDa.
ab187643 Anti-CDC42 antibody [EPR15620] was shown to specifically react with CDC42 in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266522 (knockout cell lysate ab256868) was used. Wild-type and CDC42 knockout samples were subjected to SDS-PAGE. ab187643 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 5000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Immunocytochemistry/Immunofluorescence analysis of U937 (Human histiocytic lymphoma cell line) labelling CDC42 with purified ab187643 at 1/500. Cells were fixed with 4% PFA and permeabilized with 0.1% triton X-100. ab150077 Goat anti rabbit IgG (Alexa Fluor® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.
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All lanes : Anti-CDC42 antibody [EPR15620] (ab187643) at 1/5000 dilution
Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : CDC42 knockout HAP1 cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 21 kDaLanes 1 - 4: Merged signal (red and green). Green - ab187643 observed at 20 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab187643 was shown to specifically react with CDC42 in wild-type HAP1 cells. No band was observed when CDC42 knockout samples were examined. Wild-type and CDC42 knockout samples were subjected to SDS-PAGE. ab187643 and ab8245 (loading control to GAPDH) were diluted at 1/5000 and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
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Lane 1 (input): HT-29 (human colorectal adenocarcinoma epithelial cell) whole cell lysate,10μg
Lane 2(+): HT-29 whole cell lysate
Lane 3(-): Rabbit monoclonal IgG (ab172730) instead of ab187643 in HT-29 whole cell lysate
Ab187643 immunoprecipitating CDC42 in HT-29 whole cell lysate. Capture antibody was used at a 1:60 dilution. For western blotting, ab187643 was used as the primary antibody at a 1:1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.Blocking and diluting buffer: 5% NFDM/TBST
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Intracellular Flow Cytometry analysis of HeLa cells (human cervix adenocarcinoma epithelial).Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Primary antibody was used at a 1/120 dilution (red). A Goat anti rabbit IgG (Alexa Fluor®488, ab150077) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (ab172730) was used as the isotype control (black). Cell without incubation with primary antibody and secondary antibody (Blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC42 antibody [EPR15620] (ab187643)
ab187643 staining CDC42 in Human lung carcinoma tissue sections by Immunohistochemistry (IHC-P- paraformaldehyde-fixed, paraffin-embedded sections). Antigen retrieval was by heat mediation using ab93684 (Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody at a 1/500 dilution. A ready to use Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Hematoxylin was used as a counter stain. Cytoplasmic staining on human lung carcinoma.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC42 antibody [EPR15620] (ab187643)
ab187643 staining CDC42 in Mouse colon tissue sections by Immunohistochemistry (IHC-P- paraformaldehyde-fixed, paraffin-embedded sections). Antigen retrieval was by heat mediation using ab93684 (Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody at a 1/500 dilution. A ready to use Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Hematoxylin was used as a counter stain. Cytoplasmic staining on mouse colon.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC42 antibody [EPR15620] (ab187643)
ab187643 staining CDC42 in Rat colon tissue sections by Immunohistochemistry (IHC-P- paraformaldehyde-fixed, paraffin-embedded sections). Antigen retrieval was by heat mediation using ab93684 (Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody at a 1/500 dilution. A ready to use Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Hematoxylin was used as a counter stain. Cytoplasmic staining on rat colon.
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All lanes : Anti-CDC42 antibody [EPR15620] (ab187643) at 1/20000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HT-29 cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa -
All lanes : Anti-CDC42 antibody [EPR15620] (ab187643) at 1/5000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse kidney lysate
Lane 3 : Mouse spleen lysate
Lane 4 : Rat spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC42 antibody [EPR15620] (ab187643)
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling CDC42 with ab187643 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (43)
ab187643 has been referenced in 43 publications.
- Huo J et al. Potential Resistance to Antineoplastic Aminated Fullerenes Mediated by M2-Like Monocyte-Derived Exosomes. Front Oncol 12:779939 (2022). PubMed: 35433481
- Biondo A & Meneses PI The Process of Filopodia Induction during HPV Infection. Viruses 14:N/A (2022). PubMed: 35746622
- Feng Y et al. Sertoli cell survival and barrier function are regulated by miR-181c/d-Pafah1b1 axis during mammalian spermatogenesis. Cell Mol Life Sci 79:498 (2022). PubMed: 36008729
- Scharler C et al. Extra-hematopoietic immunomodulatory role of the guanine-exchange factor DOCK2. Commun Biol 5:1246 (2022). PubMed: 36380073
- Wu M et al. Catalpol Regulates Oligodendrocyte Regeneration and Remyelination by Activating the GEF-Cdc42/Rac1 Signaling Pathway in EAE Mice. Evid Based Complement Alternat Med 2022:7074157 (2022). PubMed: 36482934