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    products/primary-antibodies/cdc42-antibody-m152-ab41429.pdf

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Cell Biology Cell Cycle Cell Division Cytokinesis
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Validated using a knockout cell line

Anti-CDC42 antibody [M152] (ab41429)

  • Datasheet
  • SDS
Reviews (5)Q&A (1)References (14)

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Western blot - Anti-CDC42 antibody [M152] (ab41429)
  • Western blot - Anti-CDC42 antibody [M152] (ab41429)
  • Immunocytochemistry/ Immunofluorescence - Anti-CDC42 antibody [M152] (ab41429)
  • Western blot - Anti-CDC42 antibody [M152] (ab41429)
  • Immunocytochemistry/ Immunofluorescence - Anti-CDC42 antibody [M152] (ab41429)

Key features and details

  • Mouse monoclonal [M152] to CDC42
  • Suitable for: ICC/IF, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-CDC42 antibody [M152]
    See all CDC42 primary antibodies
  • Description

    Mouse monoclonal [M152] to CDC42
  • Host species

    Mouse
  • Specificity

    The antibody detects a 21 kDa protein in human Jurkat cells and mouse brain. It does not recognize a human RhoA GST fusion protein.
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant CDC42 (Rat)

  • Positive control

    • WB: MCF7, HepG2, HAP1 and Jurkat cell lysate; Mouse brain lysate. ICC/IF: SH-SY5Y cells.
  • General notes

    Do not aliquot.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: PBS, 50% Glycerol, 0.1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    M152
  • Isotype

    IgG1
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Cytokinesis
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Small G Proteins
    • Other
    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Regulation
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Kinases/Phosphatases
    • Other
    • Cancer
    • Signal transduction
    • G protein signaling
    • Small G proteins
    • Ras family

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant Human CDC42 protein (ab87713)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab41429 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/50.
WB (3)
1/125 - 1/500. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
Notes
ICC/IF
1/50.
WB
1/125 - 1/500. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).

Target

  • Function

    Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Causes the formation of thin, actin-rich surface projections called filopodia.
  • Sequence similarities

    Belongs to the small GTPase superfamily. Rho family. CDC42 subfamily.
  • Post-translational
    modifications

    AMPylation at Tyr-32 and Thr-35 are mediated by bacterial enzymes in case of infection by H.somnus and V.parahaemolyticus, respectively. AMPylation occurs in the effector region and leads to inactivation of the GTPase activity by preventing the interaction with downstream effectors, thereby inhibiting actin assembly in infected cells. It is unclear whether some human enzyme mediates AMPylation; FICD has such ability in vitro but additional experiments remain to be done to confirm results in vivo.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession P60953 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 998 Human
    • Entrez Gene: 12540 Mouse
    • Entrez Gene: 64465 Rat
    • Omim: 116952 Human
    • SwissProt: P60953 Human
    • SwissProt: P60766 Mouse
    • SwissProt: Q8CFN2 Rat
    • Unigene: 467637 Human
    • Unigene: 1022 Mouse
    • Unigene: 447553 Mouse
    • Unigene: 60067 Rat
    see all
  • Form

    There are 2 isoforms produced by alternative splicing. Isoform 1 also known as: Brain; Isoform 2 also known as: Placental.
  • Alternative names

    • CDC42 antibody
    • CDC42_HUMAN antibody
    • CDC42Hs antibody
    • Cell division control protein 42 homolog antibody
    • Cell division cycle 42 (GTP binding protein 25kDa) antibody
    • Cell division cycle 42 antibody
    • dJ224A6.1.1 (cell division cycle 42 (GTP-binding protein, 25kD)) antibody
    • dJ224A6.1.2 (cell division cycle 42 (GTP-binding protein, 25kD)) antibody
    • G25K antibody
    • G25K GTP-binding protein antibody
    • Growth regulating protein antibody
    • GTP binding protein 25kDa antibody
    • Small GTP binding protein CDC42 antibody
    • TKS antibody
    see all

Images

  • Western blot - Anti-CDC42 antibody [M152] (ab41429)
    Western blot - Anti-CDC42 antibody [M152] (ab41429)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: CDC42 knockout HAP1 cell lysate (20 µg)
    Lane 3: MCF7 cell lysate (20 µg)
    Lane 4: HepG2 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab41429 observed at 20 kDa. Red - loading control, ab181602, observed at 37 kDa.

    ab41429 was shown to specifically react with CDC42 in wild-type HAP1 cells. No band was observed when CDC42 knockout samples were examined. Wild-type and CDC42 knockout samples were subjected to SDS-PAGE. ab41429 and ab181602 (loading control to GAPDH) were diluted at 1/125 and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-CDC42 antibody [M152] (ab41429)
    Western blot - Anti-CDC42 antibody [M152] (ab41429)
    Lanes 1 & 3 : Anti-CDC42 antibody [M152] (ab41429) at 1/125 dilution
    Lanes 2 & 4 : Anti-CDC42 antibody [M152] (ab41429) at 1/500 dilution

    Lanes 1-2 : human jurkat cells
    Lanes 3-4 : mouse brain

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 21 kDa
    Observed band size: 21 kDa



    Membrane was incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween 20 for 1 hour at room temperature.
  • Immunocytochemistry/ Immunofluorescence - Anti-CDC42 antibody [M152] (ab41429)
    Immunocytochemistry/ Immunofluorescence - Anti-CDC42 antibody [M152] (ab41429)Image from Haebig K et al, PLoS One. 2010 Oct 29;5(10):e13762, Fig 2. doi:10.1371/journal.pone.0013762

    ab41429 staining CDC42 in SH-SY5Y cells by Immunocytochemistry/ Immunofluorescence. SH-SY5Y cells were differentiated 6 days with 10 µM retinoic acid and fixed with 4% paraformaldehyde in PBS. After 5 minutes incubation with ice-cold methanol the cells were washed twice with PBS and blocked with 10% normal donkey serum for 30 minutes. Incubation with primary antibody was performed over night at 4°C. After three-times washing with PBS, 1 hour incubation with the labeled Cy3 secondary antibody at 37°C the cells were mounted with DABCO/Mowiol.

  • Western blot - Anti-CDC42 antibody [M152] (ab41429)
    Western blot - Anti-CDC42 antibody [M152] (ab41429)
    All lanes : Anti-CDC42 antibody [M152] (ab41429) at 1/500 dilution

    Lane 1 : Rabbit heart tissue lysate at 10 µg
    Lane 2 : Rabbit heart tissue lysate at 20 µg
    Lane 3 : Rabbit heart tissue lysate at 40 µg
    Lane 4 : Rabbit heart tissue lysate at 60 µg
    Lane 5 : Rabbit heart tissue lysate at 80 µg
    Lane 6 : Rabbit heart tissue lysate at 100 µg

    Secondary
    All lanes : Donkey anti-mouse IgG (HRP) at 1/50000 dilution

    Developed using the ECL technique.

    Predicted band size: 21 kDa

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-CDC42 antibody [M152] (ab41429)
    Immunocytochemistry/ Immunofluorescence - Anti-CDC42 antibody [M152] (ab41429)

    PC-12 (Rat adrenal gland pheochromocytoma cell line) cells grown for 4 days on poly-D-lysine-coated plates in the rpesence (200 ng/ml) or absence (control) of Nerve Growth Factor (NGF) labeling CDC42 with ab41429 in ICC/IF. Primary antibody was used at 1/50 dilution followed by a donkey anti-mouse secondaty antibody (Cy2).

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (14)

Publishing research using ab41429? Please let us know so that we can cite the reference in this datasheet.

ab41429 has been referenced in 14 publications.

  • Zhou Y  et al. Congenital biliary atresia is correlated with disrupted cell junctions and polarity caused by Cdc42 insufficiency in the liver. Theranostics 11:7262-7275 (2021). PubMed: 34158849
  • López-Ureña D  et al. Toxin B Variants from Clostridium difficile Strains VPI 10463 and NAP1/027 Share Similar Substrate Profile and Cellular Intoxication Kinetics but Use Different Host Cell Entry Factors. Toxins (Basel) 11:N/A (2019). PubMed: 31212980
  • Zhang Q  et al. miR-185 inhibits cell migration and invasion of hepatocellular carcinoma through CDC42. Oncol Lett 16:3101-3107 (2018). PubMed: 30127901
  • Javadi A  et al. PTEN controls glandular morphogenesis through a juxtamembrane ß-Arrestin1/ARHGAP21 scaffolding complex. Elife 6:N/A (2017). PubMed: 28749339
  • Quesada-Gómez C  et al. Analysis of TcdB Proteins within the Hypervirulent Clade 2 Reveals an Impact of RhoA Glucosylation on Clostridium difficile Proinflammatory Activities. Infect Immun 84:856-65 (2016). PubMed: 26755157
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-6 of 6 Abreviews or Q&A

IHC - Wholemount abreview for Anti-CDC42 antibody [M152]

Average
Abreviews
Abreviews
abreview image
Application
IHC - Wholemount
Sample
Fruit fly (Drosophila melanogaster) Tissue (Egg Chamber (Ovary))
Specification
Egg Chamber (Ovary)
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted May 30 2014

Western blot abreview for Anti-CDC42 antibody [M152]

Inconclusive
Abreviews
Abreviews
Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing (4-12% gradient)
Sample
Fruit fly (Drosophila melanogaster) Tissue lysate - whole (Whole tissue lysate from ovary)
Specification
Whole tissue lysate from ovary
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted May 30 2014

Immunohistochemistry (Frozen sections) abreview for Anti-CDC42 antibody [M152]

Average
Abreviews
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (embryo)
Specification
embryo
Fixative
Paraformaldehyde
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 05 2013

Western blot abreview for Anti-CDC42 antibody [M152]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rabbit Tissue lysate - whole (heart)
Loading amount
40 µg
Specification
heart
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 23 2010

Western blot abreview for Anti-CDC42 antibody [M152]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Vero cells)
Loading amount
20000 cells
Specification
Vero cells
Treatment
cells expressing recombitant viral protein
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 10 minute(s) · Concentration: 0.5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 09 2008

Question

I'm trying to find a monoclonal Cdc42 antibody for detection of Cdc42 with western blot. Do you know if the antibody ab41429 shows any cross-reactivity with the small GTPase Rac1?

Read More

Abcam community

Verified customer

Asked on Apr 19 2013

Answer

ab41429 does not detect RhoA, we have tested this directly using recombinant RhoA protein versus recombinant CDC42 protein. However, we have not tested reactivity to Rac1 and therefore, we cannot comment on the cross-reactivity with Rac1 since the epitope of this antibody has not been determined.

We do however have Anti-CDC42 antibody (ab64533) in the catalogue who's immunogen has only 42% homology with Rac1. This therefore suggests that ab64533 will not cross-react with Rac1. I would therefore suggest ab64533 over 41429 although we cannot guarantee that it will definitely not cross-react with Rac1.

Read More

Abcam Scientific Support

Answered on Apr 19 2013

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