Anti-CDC42 antibody [M152] (ab41429)
Key features and details
- Mouse monoclonal [M152] to CDC42
- Suitable for: ICC/IF, WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
-
Product name
Anti-CDC42 antibody [M152]
See all CDC42 primary antibodies -
Description
Mouse monoclonal [M152] to CDC42 -
Host species
Mouse -
Specificity
The antibody detects a 21 kDa protein in human Jurkat cells and mouse brain. It does not recognize a human RhoA GST fusion protein. -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant CDC42 (Rat)
-
Positive control
- WB: MCF7, HepG2, HAP1 and Jurkat cell lysate; Mouse brain lysate. ICC/IF: SH-SY5Y cells.
-
General notes
Do not aliquot.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
M152 -
Isotype
IgG1 -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab41429 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
1/50.
|
|
WB | (3) |
1/125 - 1/500. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
|
Notes |
---|
ICC/IF
1/50. |
WB
1/125 - 1/500. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa). |
Target
-
Function
Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Causes the formation of thin, actin-rich surface projections called filopodia. -
Sequence similarities
Belongs to the small GTPase superfamily. Rho family. CDC42 subfamily. -
Post-translational
modificationsAMPylation at Tyr-32 and Thr-35 are mediated by bacterial enzymes in case of infection by H.somnus and V.parahaemolyticus, respectively. AMPylation occurs in the effector region and leads to inactivation of the GTPase activity by preventing the interaction with downstream effectors, thereby inhibiting actin assembly in infected cells. It is unclear whether some human enzyme mediates AMPylation; FICD has such ability in vitro but additional experiments remain to be done to confirm results in vivo. -
Cellular localization
Cell membrane. - Information by UniProt
-
Database links
- Entrez Gene: 998 Human
- Entrez Gene: 12540 Mouse
- Entrez Gene: 64465 Rat
- Omim: 116952 Human
- SwissProt: P60953 Human
- SwissProt: P60766 Mouse
- SwissProt: Q8CFN2 Rat
- Unigene: 467637 Human
see all -
Form
There are 2 isoforms produced by alternative splicing. Isoform 1 also known as: Brain; Isoform 2 also known as: Placental. -
Alternative names
- CDC42 antibody
- CDC42_HUMAN antibody
- CDC42Hs antibody
see all
Images
-
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: CDC42 knockout HAP1 cell lysate (20 µg)
Lane 3: MCF7 cell lysate (20 µg)
Lane 4: HepG2 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab41429 observed at 20 kDa. Red - loading control, ab181602, observed at 37 kDa.ab41429 was shown to specifically react with CDC42 in wild-type HAP1 cells. No band was observed when CDC42 knockout samples were examined. Wild-type and CDC42 knockout samples were subjected to SDS-PAGE. ab41429 and ab181602 (loading control to GAPDH) were diluted at 1/125 and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
-
Lanes 1 & 3 : Anti-CDC42 antibody [M152] (ab41429) at 1/125 dilution
Lanes 2 & 4 : Anti-CDC42 antibody [M152] (ab41429) at 1/500 dilution
Lanes 1-2 : human jurkat cells
Lanes 3-4 : mouse brain
Lysates/proteins at 20 µg per lane.
Predicted band size: 21 kDa
Observed band size: 21 kDa
Membrane was incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween 20 for 1 hour at room temperature. -
Immunocytochemistry/ Immunofluorescence - Anti-CDC42 antibody [M152] (ab41429)Image from Haebig K et al, PLoS One. 2010 Oct 29;5(10):e13762, Fig 2. doi:10.1371/journal.pone.0013762
ab41429 staining CDC42 in SH-SY5Y cells by Immunocytochemistry/ Immunofluorescence. SH-SY5Y cells were differentiated 6 days with 10 µM retinoic acid and fixed with 4% paraformaldehyde in PBS. After 5 minutes incubation with ice-cold methanol the cells were washed twice with PBS and blocked with 10% normal donkey serum for 30 minutes. Incubation with primary antibody was performed over night at 4°C. After three-times washing with PBS, 1 hour incubation with the labeled Cy3 secondary antibody at 37°C the cells were mounted with DABCO/Mowiol.
-
All lanes : Anti-CDC42 antibody [M152] (ab41429) at 1/500 dilution
Lane 1 : Rabbit heart tissue lysate at 10 µg
Lane 2 : Rabbit heart tissue lysate at 20 µg
Lane 3 : Rabbit heart tissue lysate at 40 µg
Lane 4 : Rabbit heart tissue lysate at 60 µg
Lane 5 : Rabbit heart tissue lysate at 80 µg
Lane 6 : Rabbit heart tissue lysate at 100 µg
Secondary
All lanes : Donkey anti-mouse IgG (HRP) at 1/50000 dilution
Developed using the ECL technique.
Predicted band size: 21 kDa
-
PC-12 (Rat adrenal gland pheochromocytoma cell line) cells grown for 4 days on poly-D-lysine-coated plates in the rpesence (200 ng/ml) or absence (control) of Nerve Growth Factor (NGF) labeling CDC42 with ab41429 in ICC/IF. Primary antibody was used at 1/50 dilution followed by a donkey anti-mouse secondaty antibody (Cy2).
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (16)
ab41429 has been referenced in 16 publications.
- Amoah A et al. Aging of human hematopoietic stem cells is linked to changes in Cdc42 activity. Haematologica 107:393-402 (2022). PubMed: 33440922
- Heib T et al. RhoA/Cdc42 signaling drives cytoplasmic maturation but not endomitosis in megakaryocytes. Cell Rep 35:109102 (2021). PubMed: 33979620
- Zhou Y et al. Congenital biliary atresia is correlated with disrupted cell junctions and polarity caused by Cdc42 insufficiency in the liver. Theranostics 11:7262-7275 (2021). PubMed: 34158849
- López-Ureña D et al. Toxin B Variants from Clostridium difficile Strains VPI 10463 and NAP1/027 Share Similar Substrate Profile and Cellular Intoxication Kinetics but Use Different Host Cell Entry Factors. Toxins (Basel) 11:N/A (2019). PubMed: 31212980
- Zhang Q et al. miR-185 inhibits cell migration and invasion of hepatocellular carcinoma through CDC42. Oncol Lett 16:3101-3107 (2018). PubMed: 30127901