Anti-CDC42 antibody [M430] (ab202280)
![Western blot - Anti-CDC42 antibody [M430] (ab202280)](https://www.abcam.com/ps/products/202/ab202280/Images/ab202280-260582-ab202280KOWB.jpg "Western blot - Anti-CDC42 antibody [M430] (ab202280)")
Key features and details
- Mouse monoclonal [M430] to CDC42
- Suitable for: WB, ICC/IF
- Knockout validated
- Reacts with: Mouse, Rat, Human
- Isotype: IgM
Overview
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Product name
Anti-CDC42 antibody [M430]
See all CDC42 primary antibodies -
Description
Mouse monoclonal [M430] to CDC42 -
Host species
Mouse -
Specificity
ab202280 does not recognize a full length human RhoA GST fusion protein. -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Chicken, Cow, Dog, Pig, Drosophila melanogaster, Cynomolgus monkey
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Immunogen
Recombinant full length protein corresponding to Rat CDC42 aa 1 to the C-terminus.
Database link: Q8CFN2 -
Positive control
- Jurkat cells and mouse brain lysates; rat A7r5 cells; recombinant full length rat Cdc42 fusion protein.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol, 0.1% BSA, 49% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
M430 -
Isotype
IgM -
Research areas
Associated products
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Compatible Secondaries
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab202280 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500. Predicted molecular weight: 21 kDa.
|
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| ICC/IF |
1/200.
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| Notes |
|---|
|
WB
1/500. Predicted molecular weight: 21 kDa. |
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ICC/IF
1/200. |
Target
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Function
Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Causes the formation of thin, actin-rich surface projections called filopodia. -
Sequence similarities
Belongs to the small GTPase superfamily. Rho family. CDC42 subfamily. -
Post-translational
modificationsAMPylation at Tyr-32 and Thr-35 are mediated by bacterial enzymes in case of infection by H.somnus and V.parahaemolyticus, respectively. AMPylation occurs in the effector region and leads to inactivation of the GTPase activity by preventing the interaction with downstream effectors, thereby inhibiting actin assembly in infected cells. It is unclear whether some human enzyme mediates AMPylation; FICD has such ability in vitro but additional experiments remain to be done to confirm results in vivo. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 395917 Chicken
- Entrez Gene: 532712 Cow
- Entrez Gene: 101925934 Cynomolgus monkey
- Entrez Gene: 403934 Dog
- Entrez Gene: 32981 Drosophila melanogaster
- Entrez Gene: 998 Human
- Entrez Gene: 12540 Mouse
- Entrez Gene: 780428 Pig
see all -
Form
There are 2 isoforms produced by alternative splicing. Isoform 1 also known as: Brain; Isoform 2 also known as: Placental. -
Alternative names
- CDC42 antibody
- CDC42_HUMAN antibody
- CDC42Hs antibody
see all
Images
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Western blot - Anti-CDC42 antibody [M430] (ab202280)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: CDC42 knockout HAP1 cell lysate (20 µg)
Lane 3: MCF7 cell lysate (20 µg)
Lane 4: HepG2 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab202280 observed at 20 kDa. Red - loading control, ab181602, observed at 37 kDa.ab202280 was shown to recognize CDC42 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when CDC42 knockout samples were examined. Wild-type and CDC42 knockout samples were subjected to SDS-PAGE. ab202280 and ab181602 (loading control to GAPDH) were diluted at 1/500 and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-CDC42 antibody [M430] (ab202280)](https://www.abcam.com/ps/products/202/ab202280/Images/ab202280-243556-ab202280WB2.jpg)
Western blot - Anti-CDC42 antibody [M430] (ab202280)All lanes : Anti-CDC42 antibody [M430] (ab202280) at 1/500 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : mouse brain lysate
Lane 3 : Recombiant full length rat Cdc42 protein
Predicted band size: 21 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-CDC42 antibody [M430] (ab202280)](https://www.abcam.com/ps/products/202/ab202280/Images/ab202280-243555-ab2022802.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CDC42 antibody [M430] (ab202280)Immunofluorescence analysis of paraformaldehyde-fixed rat A7r5 cells, labeling CDC42 using ab202280 at a 1/200 dilution, in the absence (left, black protein block) or presence (right, red image) of full length Cdc42 recombinant protein. Goat anti-mouse conjugated to DyLight® 594 was used as secondary antibody.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab202280 has not yet been referenced specifically in any publications.