Recombinant Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237)
![Western blot - Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237)](https://www.abcam.com/ps/products/192/ab192237/Images/ab192237-230013-anti-cdca5-antibody-epr16331-c-terminal-western-blot.jpg "Western blot - Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16331] to CDCA5 - C-terminal
- Suitable for: ICC/IF, WB, IHC-P, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CDCA5 antibody [EPR16331] - C-terminal
See all CDCA5 primary antibodies -
Description
Rabbit monoclonal [EPR16331] to CDCA5 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-P, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- 293, HeLa, Jurkat and SH-SY5Y cell lysates; Human stomach and human endometrium adenocarcinoma tissues; HeLa and 293 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16331 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab192237 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/100.
|
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| WB |
1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 28 kDa).
|
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
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| Flow Cyt (Intra) |
1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
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ICC/IF
1/100. |
|
WB
1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 28 kDa). |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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Flow Cyt (Intra)
1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Regulator of sister chromatid cohesion in mitosis stabilizing cohesin complex association with chromatin. May antagonize the action of WAPAL which stimulates cohesin dissociation from chromatin. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair. Required for efficient DNA double-stranded break repair. -
Sequence similarities
Belongs to the sororin family. -
Domain
The KEN box is required for the association with the APC/C complex. -
Post-translational
modificationsPhosphorylated. Phosphorylation, as cells enter mitosis, disrupts the interaction with PDS5A and relieves the inhibition of WAPAL by CDCA5.
Ubiquitinated by the APC/C complex in G1, leading to its degradation. -
Cellular localization
Nucleus. Chromosome. Cytoplasm. Associates with nuclear chromatin from S phase until metaphase and is released in the cytoplasm upon nuclear envelope breakdown. - Information by UniProt
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Database links
- Entrez Gene: 113130 Human
- Omim: 609374 Human
- SwissProt: Q96FF9 Human
- Unigene: 434886 Human
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Alternative names
- CDCA5 antibody
- CDCA5_HUMAN antibody
- Cell division cycle associated protein 5 antibody
see all
Images
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Western blot - Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237)Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237) at 1/1000 dilution + SH-SY5Y cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 28 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237)](https://www.abcam.com/ps/products/192/ab192237/Images/ab192237-253279-anti-cdca5-antibody-epr16331-c-terminal-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237)Immunofluorescent analysis of HeLa cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling CDCA5 with ab192237 at 1/100 dilution (5μg/mL) followed by Goat anti rabbit IgG (AlexaFluor® 488) (ab150077) secondary at 1/200 dilution and counter-stained with DAPI (blue).
Negative controls: anti-CDCA5 at 1/100 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) at 1/400 dilution. -
![Flow Cytometry (Intracellular) - Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237)](https://www.abcam.com/ps/products/192/ab192237/Images/ab192237-230017-anti-cdca5-antibody-epr16331-c-terminal-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237)Intracellular flow cytometric analysis of 293 cells (paraformaldehyde-fixed, 2%) labeling CDCA5with ab192237 at 1/100 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237)](https://www.abcam.com/ps/products/192/ab192237/Images/ab192237-230015-anti-cdca5-antibody-epr16331-c-terminal-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237)Immunohistochemical analysis of paraffin-embedded Human endometrium adenocarcinoma tissue labeling CDCA5 with ab192237 at 1/100 (5 µg/ml) dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237)](https://www.abcam.com/ps/products/192/ab192237/Images/ab192237-230014-anti-cdca5-antibody-epr16331-c-terminal-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237)Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling CDCA5 with ab192237 at 1/100 (5 µg/ml) dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Western blot - Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237)](https://www.abcam.com/ps/products/192/ab192237/Images/ab192237-230012-anti-cdca5-antibody-epr16331-c-terminal-western-blot.jpg)
Western blot - Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237)All lanes : Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237) at 1/10000 dilution
Lane 1 : 293 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 28 kDa -
![Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237)](https://www.abcam.com/ps/products/192/ab192237/Images/ab192237-8-benefits-of-recombinant-antibodies.png)
Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (14)
ab192237 has been referenced in 14 publications.
- Huang X et al. Loss of cell division cycle‑associated 5 promotes cell apoptosis by activating DNA damage response in clear cell renal cell carcinoma. Int J Oncol 61:N/A (2022). PubMed: 35642672
- Ji J et al. CDCA5 promotes the progression of prostate cancer by affecting the ERK signalling pathway. Oncol Rep 45:921-932 (2021). PubMed: 33650660
- Zhou C et al. The cohesin stabilizer Sororin drives G2-M transition and spindle assembly in mammalian oocytes. Sci Adv 7:eabg9335 (2021). PubMed: 34559563
- He X et al. USP13 interacts with cohesin and regulates its ubiquitination in human cells. J Biol Chem 296:100194 (2021). PubMed: 33334891
- Luo Z et al. SPOP promotes CDCA5 degradation to regulate prostate cancer progression via the AKT pathway. Neoplasia 23:1037-1047 (2021). PubMed: 34509929