Recombinant Anti-CDK1 (phospho T161) + CDK2 / CDK3 (phospho T160) antibody [EPR19546] (ab201008)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19546] to CDK1 (phospho T161) + CDK2 (phospho T160) + CDK3 (phospho T160)
- Suitable for: WB, Dot blot, IHC-P, IP, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-CDK1 (phospho T161) + CDK2 / CDK3 (phospho T160) antibody [EPR19546] -
Description
Rabbit monoclonal [EPR19546] to CDK1 (phospho T161) + CDK2 (phospho T160) + CDK3 (phospho T160) -
Host species
Rabbit -
Specificity
ab201008 also recognizes CDK2 (phospho T160) and CDK3 (phospho T160). -
Tested applications
Suitable for: WB, Dot blot, IHC-P, IP, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa treated with UV for 90 minutes, HeLa treated with UV for 90 minutes then with alkaline phosphatase for 1 hour, C6, C6 treated with alkaline phosphatase for 1 hour and NIH/3T3 whole cell lysate. IHC-P: Human tonsil and cervix cancer tissues; Rat testis tissue. ICC/IF: HeLa cells. IP: HeLa treated with 25J/m2 UV for 1-hour whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19546 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Buffer
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab201008 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 34, 28 kDa (predicted molecular weight: 34 kDa).
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Dot blot |
1/1000.
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IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Recommended for human and rat only. |
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IP |
1/30.
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ICC/IF |
1/100.
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Notes |
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WB
1/1000. Detects a band of approximately 34, 28 kDa (predicted molecular weight: 34 kDa). |
Dot blot
1/1000. |
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. Recommended for human and rat only. |
IP
1/30. |
ICC/IF
1/100. |
Target
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Cellular localization
CDK1: Nucleus. -
Database links
- Entrez Gene: 1017 Human
- Entrez Gene: 1018 Human
- Entrez Gene: 983 Human
- Entrez Gene: 12534 Mouse
- Entrez Gene: 12566 Mouse
- Entrez Gene: 362817 Rat
- Entrez Gene: 54237 Rat
- Omim: 116940 Human
see all -
Form
CDK1: CDK1 can be located to the Nucleus, cytoplasm and Mithocondria. It's cytoplasmic during interphase and reversibly translocated from cytoplasm to the nucleus when phosphorilated before G2-M transition when associated with cyclin-B1. Accumulates in mitochondria in G2-arrested cells upon DNA-damage.
Images
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All lanes : Anti-CDK1 (phospho T161) + CDK2 / CDK3 (phospho T160) antibody [EPR19546] (ab201008) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HeLa treated with UV for 90 minutes whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 34 kDa
Observed band size: 28,34 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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Dot blot analysis of CDK1 (phospho T161) labeled with ab201008 at 1/1000 dilution.
Lane 1: CDK1 (phospho T161) phospho peptide.
Lane 2: CDK1 non-phospho peptide.Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
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Dot blot analysis of CDK2 (phospho T160) labeled with ab201008 at 1/1000 dilution.
Lane 1: CDK2 (phospho T160) phospho peptide.
Lane 2: CDK2 non-phospho peptide.Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
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Dot blot analysis of CDK3 (phospho T160) labeled with ab201008 at 1/1000 dilution.
Lane 1: CDK3 (phospho T160) phospho peptide.
Lane 2: CDK3 non-phospho peptide.Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
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All lanes : Anti-CDK1 (phospho T161) + CDK2 / CDK3 (phospho T160) antibody [EPR19546] (ab201008) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HeLa treated with UV for 90 minutes whole cell lysate
Lane 3 : HeLa treated with UV for 90 minutes then with alkaline phosphatase for 1-hour whole cell lysate
Lane 4 : HeLa treated with UV for 90 minutes then with alkaline phosphatase overnight whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 34 kDa
Observed band size: 28,34 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-CDK1 (phospho T161) + CDK2 / CDK3 (phospho T160) antibody [EPR19546] (ab201008) at 1/1000 dilution
Lane 1 : C6 (rat glial tumor cell line) whole cell lysate
Lane 2 : C6 treated with alkaline phosphatase for 1 hour whole cell lysate
Lane 3 : C6 treated with alkaline phosphatase overnight whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 34 kDa
Observed band size: 28,34 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-CDK1 (phospho T161) + CDK2 / CDK3 (phospho T160) antibody [EPR19546] (ab201008) at 1/1000 dilution
Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 2 : C6 (rat glial tumor cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 34 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
Exposure time: 3 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK1 (phospho T161) + CDK2 / CDK3 (phospho T160) antibody [EPR19546] (ab201008)
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CDK1 (phospho T161) with ab201008 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus and weak cytoplasm staining of germinal center from human tonsil is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK1 (phospho T161) + CDK2 / CDK3 (phospho T160) antibody [EPR19546] (ab201008)
Immunohistochemical analysis of paraffin-embedded human cervix cancer tissue labeling CDK1 (phospho T161) with ab201008 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus and cytoplasm staining of cancer cells from human cervix cancer is observed [PMID: 15623629]. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK1 (phospho T161) + CDK2 / CDK3 (phospho T160) antibody [EPR19546] (ab201008)
Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling CDK1 (phospho T161) with ab201008 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus and cytoplasm staining of rat testis is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunocytochemistry/ Immunofluorescence - Anti-CDK1 (phospho T161) + CDK2 / CDK3 (phospho T160) antibody [EPR19546] (ab201008)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling CDK1 (phospho T161) with ab201008 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing specific signal in M phase cells. The signal decreased after treatment with lambda protein phosphatase 31°C for 5 hours.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-Alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
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Immunoprecipitation - Anti-CDK1 (phospho T161) + CDK2 / CDK3 (phospho T160) antibody [EPR19546] (ab201008)
CDK1 (phospho T161) was immunoprecipitated from 0.35mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab201008 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab201008 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: HeLa treated with 25J/m2 UV for 1 hour whole cell lysate 10 µg (Input).
Lane 2: ab201008 IP in HeLa treated with 25J/m2 UV for 1 hour whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab201008 in HeLa treated with 25J/m2 UV for 1 hour whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (10)
ab201008 has been referenced in 10 publications.
- Xia X et al. Neddylation of HER2 Inhibits its Protein Degradation and promotes Breast Cancer Progression. Int J Biol Sci 19:377-392 (2023). PubMed: 36632463
- Shen E et al. DEP domain containing 1B (DEPDC1B) exerts the tumor promoter in hepatocellular carcinoma through activating p53 signaling pathway via kinesin family member 23 (KIF23). Bioengineered 13:1103-1114 (2022). PubMed: 34983303
- Wang D et al. A noncoding regulatory RNA Gm31932 induces cell cycle arrest and differentiation in melanoma via the miR-344d-3-5p/Prc1 (and Nuf2) axis. Cell Death Dis 13:314 (2022). PubMed: 35393397
- Zhang Y et al. LncRNA-CCDC144NL-AS1 Promotes the Development of Hepatocellular Carcinoma by Inducing WDR5 Expression via Sponging miR-940. J Hepatocell Carcinoma 8:333-348 (2021). PubMed: 33977095
- Hu S et al. LINCS gene expression signature analysis revealed bosutinib as a radiosensitizer of breast cancer cells by targeting eIF4G1. Int J Mol Med 47:N/A (2021). PubMed: 33693953
- Zhao J et al. lncRNA LIFR-AS1 inhibits gastric carcinoma cell proliferation, migration and invasion by sponging miR-4698. Mol Med Rep 23:N/A (2021). PubMed: 33355363
- Yang T et al. Punicalin Alleviates OGD/R-Triggered Cell Injury via TGF-ß-Mediated Oxidative Stress and Cell Cycle in Neuroblastoma Cells SH-SY5Y. Evid Based Complement Alternat Med 2021:6671282 (2021). PubMed: 33628309
- Huang W et al. Blocking the short isoform of augmenter of liver regeneration inhibits proliferation of human multiple myeloma U266 cells via the MAPK/STAT3/cell cycle signaling pathway. Oncol Lett 21:197 (2021). PubMed: 33574936
- Chen X et al. Incaspitolide A extracted from Carpesium cernuum induces apoptosis in vitro via the PI3K/AKT pathway in benign prostatic hyperplasia. Biosci Rep 41:N/A (2021). PubMed: 34100062
- He P et al. AMPK Activity Contributes to G2 Arrest and DNA Damage Decrease via p53/p21 Pathways in Oxidatively Damaged Mouse Zygotes. Front Cell Dev Biol 8:539485 (2020). PubMed: 33015052