Recombinant Anti-CDK5RAP3 antibody [EPR11294(B)] (ab157203)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK5RAP3 antibody [EPR11294(B)] (ab157203)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR11294(B)] to CDK5RAP3
Suitable for: WB, IP, IHC-P, Flow Cyt (Intra)
Reacts with: Mouse, Human

Carrier Free

Product name

Anti-CDK5RAP3 antibody [EPR11294(B)]
See all CDK5RAP3 primary antibodies
Description

Rabbit monoclonal [EPR11294(B)] to CDK5RAP3
Host species

Rabbit
Specificity

The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse.
Tested applications

Suitable for: WB, IP, IHC-P, Flow Cyt (Intra)more details
Unsuitable for: ICC/IF
Species reactivity

Reacts with: Mouse, Human
Immunogen

Synthetic peptide within Human CDK5RAP3 aa 450 to the C-terminus. The exact sequence is proprietary.
Database link: Q96JB5
Positive control
- WB: Jurkat, HeLa, A431, CTLL-2, EL4, H9 and 293T cell lysates. IHC-P: Human pancreas and stomach tissue. Flow Cyt (intra): HEK-293T cells. IP: CDK5RAP3 IP in HEK-293T cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR11294(B)
Isotype

IgG
Research areas
- Cancer
- Cell Death
- Apoptosis
- Apoptosis Markers
- NFkB
- RelA (p65)

Alternative Versions
- Anti-CDK5RAP3 antibody [EPR11294(B)] - BSA and Azide free (ab242399)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
Recombinant Protein
- Recombinant Human CDK5RAP3 protein (ab201715)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab157203 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000 - 1/10000. Predicted molecular weight: 57 kDa.
IP		1/10 - 1/100.
IHC-P		1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse.
Flow Cyt (Intra)		1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 57 kDa.
IP 1/10 - 1/100.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse.
Flow Cyt (Intra) 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Application notes

Is unsuitable for ICC/IF.

Function

Potential regulator of CDK5 activity. May be involved in cell proliferation. Regulates CDK5 activity via its interaction with CDK5R1.
Tissue specificity

Ubiquitous. Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform 3 is expressed in kidney, liver, skeletal muscle and placenta.
Sequence similarities

Belongs to the CDK5RAP3 family.
Post-translational
modifications

Phosphorylated in vitro by CDK5.
Target information above from: UniProt accession Q96JB5 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 80279 Human
- Entrez Gene: 80280 Mouse
- Omim: 608202 Human
- SwissProt: Q96JB5 Human
- SwissProt: Q99LM2 Mouse
- Unigene: 20157 Human
- Unigene: 28297 Mouse
Alternative names
- C53 antibody
- CDK5 activator binding protein C53 antibody
- CDK5 activator-binding protein C53 antibody
- CDK5 regulatory subunit associated protein 3 antibody
- CDK5 regulatory subunit-associated protein 3 antibody
- Cdk5rap3 antibody
- CK5P3_HUMAN antibody
- HSF 27 antibody
- HSF27 antibody
- IC53 antibody
- Ischemic heart CDK5 activator binding protein C53 antibody
- LXXLL/leucine zipper containing ARFbinding protein antibody
- LZAP antibody
- MST016 antibody
- OK/SW cl.114 antibody
- PP1553 antibody
- Protein HSF-27 antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK5RAP3 antibody [EPR11294(B)] (ab157203)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human stomach tissue sections labeling CDK5RAP3 with purified ab157203 at 1/50 dilution (2.8 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Western blot - Anti-CDK5RAP3 antibody [EPR11294(B)] (ab157203)

All lanes : Anti-CDK5RAP3 antibody [EPR11294(B)] (ab157203) at 1/2000 dilution (Purified)

Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 3 : A431 (Human epidermoid carcinoma epithelial cell) whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 57 kDa
Observed band size: 57 kDa
Western blot - Anti-CDK5RAP3 antibody [EPR11294(B)] (ab157203)

All lanes : Anti-CDK5RAP3 antibody [EPR11294(B)] (ab157203) at 1/1000 dilution (Purified)

Lane 1 : CTLL-2 (Mouse T lymphocyte) whole cell lysates
Lane 2 : EL4 (Mouse lymphoma T lymphocyte) whole cell lysates
Lane 3 : H9 (Human lymphoma T lymphocyte) whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 57 kDa
Observed band size: 57 kDa
Immunoprecipitation - Anti-CDK5RAP3 antibody [EPR11294(B)] (ab157203)

ab157203 (purified) at 1/20 dilution (1ug) immunoprecipitating CDK5RAP3 in HEK-293 whole cell lysate. HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate 10ug
Lane 2 (+): ab157203 & HEK-293 whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab157203 in HEK-293 whole cell lysate
For western blotting, VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
Flow Cytometry (Intracellular) - Anti-CDK5RAP3 antibody [EPR11294(B)] (ab157203)

Intracellular Flow Cytometry analysis of HEK-293 (Human embryonic kidney epithelial cell) cells labeling CDK5RAP3 with purified ab157203 at 1/20 dilution (10µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK5RAP3 antibody [EPR11294(B)] (ab157203)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human pancreas tissue, labeling CDK5RAP3 using ab157203 (unpurified) at a 1/50 dilution.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-CDK5RAP3 antibody [EPR11294(B)] (ab157203)

Western blot analysis of Immunoprecipitation pellet from HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate showing CDK5RAP3, using ab157203 (unpurified) at 1/10 dilution.
Western blot - Anti-CDK5RAP3 antibody [EPR11294(B)] (ab157203)

All lanes : Anti-CDK5RAP3 antibody [EPR11294(B)] (ab157203) at 1/1000 dilution (unpurified)

Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : A431 (Human epidermoid carcinoma cell line) cell lysate
Lane 4 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 57 kDa
Flow Cytometry (Intracellular) - Anti-CDK5RAP3 antibody [EPR11294(B)] (ab157203)

Intracellular Flow Cytometry analysis of permeabilized HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells labeling CDK5RAP3 (red), using ab157203 (unpurified) at a 1/10 dilution, and negative control cells probed with a Rabbit IgG (green)
Anti-CDK5RAP3 antibody [EPR11294(B)] (ab157203)

Flow cytometry protocols
Immunoprecipitation protocols
Immunohistochemistry protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab157203? Please let us know so that we can cite the reference in this datasheet.

ab157203 has been referenced in 6 publications.

Hu X et al. Ubiquitin fold modifier 1 activates NF-?B pathway by down-regulating LZAP expression in the macrophage of diabetic mouse model. Biosci Rep 40:N/A (2020). PubMed: 31829413
Yang R et al. CDK5RAP3, a UFL1 substrate adaptor, is crucial for liver development. Development 146:N/A (2019). PubMed: 30635284
Chen QY et al. CDK5RAP3 Inhibits the Translocation of MCM6 to Influence the Prognosis in Gastric Cancer. J Cancer 10:4488-4498 (2019). PubMed: 31528213
Lin JX et al. CDK5RAP3 inhibits angiogenesis in gastric neuroendocrine carcinoma by modulating AKT/HIF-1a/VEGFA signaling. Cancer Cell Int 19:282 (2019). PubMed: 31728130
Zheng CH et al. CDK5RAP3 suppresses Wnt/ß-catenin signaling by inhibiting AKT phosphorylation in gastric cancer. J Exp Clin Cancer Res 37:59 (2018). PubMed: 29540196
Lin JX et al. Low expression of CDK5RAP3 and DDRGK1 indicates a poor prognosis in patients with gastric cancer. World J Gastroenterol 24:3898-3907 (2018). PubMed: 30228783

Customer reviews and Q&As

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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Specificity

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Isotype control

Positive Controls

Recombinant Protein

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Sequence similarities

Post-translationalmodifications

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

References (6)

Customer reviews and Q&As

Post-translational
modifications