Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
RecombinantRabMAb

Recombinant Anti-Cdk9 antibody [EPR22956-60] (ab261724)

Submit a review Submit a question
Western blot - Anti-Cdk9 antibody [EPR22956-60] (ab261724)
  • Western blot - Anti-Cdk9 antibody [EPR22956-60] (ab261724)
  • Immunoprecipitation - Anti-Cdk9 antibody [EPR22956-60] (ab261724)
  • Immunoprecipitation - Anti-Cdk9 antibody [EPR22956-60] (ab261724)
  • Flow Cytometry (Intracellular) - Anti-Cdk9 antibody [EPR22956-60] (ab261724)
  • Flow Cytometry (Intracellular) - Anti-Cdk9 antibody [EPR22956-60] (ab261724)
  • Anti-Cdk9 antibody [EPR22956-60] (ab261724)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22956-60] to Cdk9
  • Suitable for: Flow Cyt (Intra), WB, IP
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-Cdk9 antibody [EPR22956-60]
    See all Cdk9 primary antibodies

  • Description

    Rabbit monoclonal [EPR22956-60] to Cdk9

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IPmore details
    Unsuitable for: ChIP,ICC/IF or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293T, HeLa, Human heart, Human spleen, C6, RAW264.7, PC-12 and NIH/3T3 lysates. Flow Cyt (intra): HeLa and NIH/3T3 cells. IP: HeLa and NIH/3T3 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab261724 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/600.
WB
1/1000. Predicted molecular weight: 43 kDa.
IP
1/30.
Notes
Flow Cyt (Intra)
1/600.
WB
1/1000. Predicted molecular weight: 43 kDa.
IP
1/30.
Application notes
Is unsuitable for ChIP,ICC/IF or IHC-P.

Target

  • Function

    Member of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to production elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II), SUPT5H and RDBP. The CDK9/cyclin-K complex has also a kinase activity toward CTD of RNAP II and can substitute for P-TEFb in vitro.

  • Tissue specificity

    Ubiquitous.

  • Sequence similarities

    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
    Contains 1 protein kinase domain.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • C-2K antibody
    • CDC2 related kinase antibody
    • CDC2L4 antibody
    • Cdk 9 antibody
    • Cdk9 antibody
    • CDK9_HUMAN antibody
    • Cell division cycle 2-like protein kinase 4 antibody
    • Cell division protein kinase 9 antibody
    • CTK1 antibody
    • Cyclin dependent kinase 9 antibody
    • Cyclin-dependent kinase 9 antibody
    • PITALRE antibody
    • Serine/threonine-protein kinase PITALRE antibody
    • TAK antibody
    • Tat associated kinase complex catalytic subunit antibody
    see all

Images

  • Western blot - Anti-Cdk9 antibody [EPR22956-60] (ab261724)
    Western blot - Anti-Cdk9 antibody [EPR22956-60] (ab261724)
    All lanes : Anti-Cdk9 antibody [EPR22956-60] (ab261724) at 1/1000 dilution

    Lane 1 : C6 (rat glial tumor glial cell), whole cell lysate
    Lane 2 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
    Lane 3 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1-2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
    Lane 3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 43 kDa
    Observed band size: 42,55 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The 2 isoforms (42KD, 55KD) of CDK9 observed is consistent with what has been described in the literature (PMID: 26766294, 15452830). 

    Exposure time: 3 minutes

     

  • Western blot - Anti-Cdk9 antibody [EPR22956-60] (ab261724)
    Western blot - Anti-Cdk9 antibody [EPR22956-60] (ab261724)
    All lanes : Anti-Cdk9 antibody [EPR22956-60] (ab261724) at 1/1000 dilution

    Lane 1 : 293T (human embryonic kidney epithelial cell), whole cell lysate
    Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 3 : Human heart tissue lysate
    Lane 4 : Human spleen tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 43 kDa
    Observed band size: 42,55 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The 2 isoforms (42KDa, 55KD) of CDK9 observed is consistent with what has been described in the literature (PMID: 26766294, 15452830). 

    Exposure time: Lanes 1-2: 7.75 seconds Lanes 3-4: 3 minutes

  • Immunoprecipitation - Anti-Cdk9 antibody [EPR22956-60] (ab261724)
    Immunoprecipitation - Anti-Cdk9 antibody [EPR22956-60] (ab261724)

    CDK9 was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate with ab261724 at 1/30 (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab261724 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 10ug

    Lane 2: ab261724 IP in NIH/3T3 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab261724 in NIH/3T3 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds

    The 2 isoforms (42KD, 55KD) of CDK9 observed is consistent with what has been described in the literature (PMID: 26766294, 15452830).

     

  • Immunoprecipitation - Anti-Cdk9 antibody [EPR22956-60] (ab261724)
    Immunoprecipitation - Anti-Cdk9 antibody [EPR22956-60] (ab261724)

    CDK9 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with ab261724 at 1/30 (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab261724 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10ug

    Lane 2: ab261724 IP in HeLa whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab261724 in HeLa whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds

    The 2 isoforms (42KD, 55KD) of CDK9 observed is consistent with what has been described in the literature (PMID: 26766294, 15452830).

     

  • Flow Cytometry (Intracellular) - Anti-Cdk9 antibody [EPR22956-60] (ab261724)
    Flow Cytometry (Intracellular) - Anti-Cdk9 antibody [EPR22956-60] (ab261724)

    Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling CDK9 with ab261724 at 1/600 (Red) compared with a Rabbit monoclonal IgG (ab172730, Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/200 was used as the secondary antibody. 

     

     

  • Flow Cytometry (Intracellular) - Anti-Cdk9 antibody [EPR22956-60] (ab261724)
    Flow Cytometry (Intracellular) - Anti-Cdk9 antibody [EPR22956-60] (ab261724)

    Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling CDK9 with ab261724 at 1/600 (Red) compared with a Rabbit monoclonal IgG (ab172730, Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/200 was used as the secondary antibody. 

     

     

  • Anti-Cdk9 antibody [EPR22956-60] (ab261724)
    Anti-Cdk9 antibody [EPR22956-60] (ab261724)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab261724? Please let us know so that we can cite the reference in this datasheet.

ab261724 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

There are currently no Customer reviews or Questions for ab261724.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com