Name: Anti-Cdk9 antibody [EPR3119Y]
Brand: RabMAb
SKU: ab76320
Rating: 3 (3 reviews)

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Western blot - Anti-Cdk9 antibody [EPR3119Y] (ab76320)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR3119Y] to Cdk9
Suitable for: WB, IP, IHC-P, ICC/IF, Flow Cyt (Intra)
Reacts with: Mouse, Rat, Human

Alexa Fluor® 488 Alexa Fluor® 647 Alkaline Phosphatase APC Carrier Free HRP PE

Product name

Anti-Cdk9 antibody [EPR3119Y]
See all Cdk9 primary antibodies
Description

Rabbit monoclonal [EPR3119Y] to Cdk9
Host species

Rabbit
Tested applications

Suitable for: WB, IP, IHC-P, ICC/IF, Flow Cyt (Intra)more details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HT-29, NIH/3T3, C6, HL-60, HeLa or 293 cell lysates. IHC-P: Human breast carcinoma, Human Bladder, mouse testis tissue. ICC/IF: HT-29 cells Flow Cyt (intra): HeLa cells IP: HeLa cells
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR3119Y
Isotype

IgG
Research areas

Alternative Versions
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant Human Cdk9 protein (ab85603)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab76320 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/5000. Predicted molecular weight: 43 kDa. For unpurified use as 1/1000 - 1/2000
IP		1/20. For unpurified use as 1/40
IHC-P		1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols.
ICC/IF	(1)	1/50. For unpurified use as 1/100 - 1/250
Flow Cyt (Intra)		1/60.

Notes
WB 1/5000. Predicted molecular weight: 43 kDa. For unpurified use as 1/1000 - 1/2000
IP 1/20. For unpurified use as 1/40
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols.
ICC/IF 1/50. For unpurified use as 1/100 - 1/250
Flow Cyt (Intra) 1/60.

Function

Member of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to production elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II), SUPT5H and RDBP. The CDK9/cyclin-K complex has also a kinase activity toward CTD of RNAP II and can substitute for P-TEFb in vitro.
Tissue specificity

Ubiquitous.
Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain.
Cellular localization

Nucleus.
Target information above from: UniProt accession P50750 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 1025 Human
- Entrez Gene: 107951 Mouse
- Entrez Gene: 362110 Rat
- Omim: 603251 Human
- SwissProt: P50750 Human
- SwissProt: Q99J95 Mouse
- SwissProt: Q641Z4 Rat
- Unigene: 150423 Human
- Unigene: 706809 Human
- Unigene: 27557 Mouse
- Unigene: 98228 Rat
see all
Alternative names
- C-2K antibody
- CDC2 related kinase antibody
- CDC2L4 antibody
- Cdk 9 antibody
- Cdk9 antibody
- CDK9_HUMAN antibody
- Cell division cycle 2-like protein kinase 4 antibody
- Cell division protein kinase 9 antibody
- CTK1 antibody
- Cyclin dependent kinase 9 antibody
- Cyclin-dependent kinase 9 antibody
- PITALRE antibody
- Serine/threonine-protein kinase PITALRE antibody
- TAK antibody
- Tat associated kinase complex catalytic subunit antibody
see all

Western blot - Anti-Cdk9 antibody [EPR3119Y] (ab76320)

All lanes : Anti-Cdk9 antibody [EPR3119Y] (ab76320) at 1/5000 dilution (Purified)

Lane 1 : HT-29 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates
Lane 3 : C6 (Rat glial tumor glial cell) whole cell lysates

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 43 kDa
Observed band size: 43 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk9 antibody [EPR3119Y] (ab76320)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat testis tissue sections labeling Cdk9 with Purified ab76320 at 1:100 dilution (1.08 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunocytochemistry/ Immunofluorescence - Anti-Cdk9 antibody [EPR3119Y] (ab76320)

Immunocytochemistry/ Immunofluorescence analysis of HT-29 (Human colorectal adenocarcinoma epithelial cell) cells labeling Cdk9 with Purified ab76320 at 1:50 dilution (2.2 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk9 antibody [EPR3119Y] (ab76320)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse testis tissue sections labeling Cdk9 with Purified ab76320 at 1:100 dilution (1.08 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk9 antibody [EPR3119Y] (ab76320)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human bladder carcinoma tissue sections labeling Cdk9 with Purified ab76320 at 1:100 dilution (1.08 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Flow Cytometry (Intracellular) - Anti-Cdk9 antibody [EPR3119Y] (ab76320)

Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Cdk9 with Purified ab76320 at 1/60 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Immunoprecipitation - Anti-Cdk9 antibody [EPR3119Y] (ab76320)

ab76320 (purified) at 1:20 dilution (0.5µg) immunoprecipitating Cdk9 in HeLa whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab76320 & HeLa whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab76320 in HeLa whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
Western blot - Anti-Cdk9 antibody [EPR3119Y] (ab76320)

All lanes : Anti-Cdk9 antibody [EPR3119Y] (ab76320) at 1/2000 dilution (Unpurified)

Lane 1 : HT-29 cell lysate
Lane 2 : HL-60 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : 293 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP at 1/1000 dilution

Predicted band size: 43 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Cdk9 antibody [EPR3119Y] (ab76320)Image courtesy of an Abreview submitted by Dr. Kirk McManus, Univ. of Manitoba/Cancer Care MICB, Canada

Unpurified ab76320 (1/200) staining Cdk9 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised in 0.5% Triton X100/ PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.
See Abreview
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk9 antibody [EPR3119Y] (ab76320)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using unpurified ab76320 at 1/100 dilution.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk9 antibody [EPR3119Y] (ab76320)

Unpurified ab76320 showing positive staining in Cervical carcinoma tissue.
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk9 antibody [EPR3119Y] (ab76320)

Unpurified ab76320 showing positive staining in Colonic adenocarcinoma tissue.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk9 antibody [EPR3119Y] (ab76320)

Unpurified ab76320 showing positive staining in Lung adenocarcinoma tissue.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk9 antibody [EPR3119Y] (ab76320)

Unpurified ab76320 showing positive staining in Normal tonsil tissue.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Anti-Cdk9 antibody [EPR3119Y] (ab76320)

Immunoprecipitation protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab76320? Please let us know so that we can cite the reference in this datasheet.

ab76320 has been referenced in 14 publications.

Borowczak J et al. The prognostic role of p53 and its correlation with CDK9 in urothelial carcinoma. Clin Transl Oncol 25:830-840 (2023). PubMed: 36374405
Guo T et al. CDK9 is up-regulated and associated with prognosis in patients with papillary thyroid carcinoma. Medicine (Baltimore) 101:e28309 (2022). PubMed: 35119000
Cheng SS et al. Inhibition of the CDK9-cyclin T1 protein-protein interaction as a new approach against triple-negative breast cancer. Acta Pharm Sin B 12:1390-1405 (2022). PubMed: 35530158
Zhang Z et al. Comprehensive bioinformatics analysis reveals kinase activity profiling associated with heart failure. J Cell Biochem N/A:N/A (2021). PubMed: 33899242
Gao W et al. Deubiquitinating Enzyme USP21 Inhibits HIV-1 Replication by Downregulating Tat Expression. J Virol 95:e0046021 (2021). PubMed: 33827943

View all Publications for this product

Customer reviews and Q&As

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1-3 of 3 Abreviews

Application

ChIP

Sample

Human Cell lysate - nuclear (HAP1 cells)

Negative control

1kb up and downstream of the TSS of the hnRNP H1 gene, an untranscribed region on chr.17 (q21.31) and 2,7 kb downstream of the TES of the Gadd45b gene.

Specification

HAP1 cells

Detection step

Real-time PCR

Type

Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: formaldehyde

Positive control

hnRNP H1, b-actin, GAPDH and myc gene promoters

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Sep 12 2017

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (HeLa)

Specification

HeLa

Fixative

Paraformaldehyde

Permeabilization

Yes - 0.5% TritonX100 in PBS

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Kirk Mcmanus

Verified customer

Submitted Oct 19 2012

Application

Western blot

Sample

Xenopus laevis Cell lysate - whole cell (Xenopus laevis gastrula embryo extract (Stage12))

Loading amount

0.5 µg

Specification

Xenopus laevis gastrula embryo extract (Stage12)

Gel Running Conditions

Reduced Denaturing (12% SDS-PAGE)

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 23°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Kei Miyamoto

Verified customer

Submitted Oct 10 2012

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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Isotype control

Recombinant Protein

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

References (14)

Customer reviews and Q&As

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