Recombinant Anti-CDX2 antibody [EPR2764Y] (ab76541)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2764Y] to CDX2
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-CDX2 antibody [EPR2764Y]
See all CDX2 primary antibodies -
Description
Rabbit monoclonal [EPR2764Y] to CDX2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt (Intra) or ICC/IF -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Rabbit -
Immunogen
Synthetic peptide within Human CDX2 aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: Q99626 -
Positive control
- WB: Caco-2, HT-29, human, mouse and rat colon cancer and human small intestine lysates. IHC-P: Human colon, gastric adenocarcinoma, colonic adenocarcinoma, intestinal tissues; Rat and mouse colon tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark. -
Dissociation constant (KD)
KD = 1.00 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2764Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab76541 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 34 kDa).
For unpurified use at 1/1000 - 1/5000 |
IHC-P | (4) |
1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
For unpurified use at 1/250 - 1/500 |
Notes |
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WB
1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 34 kDa). For unpurified use at 1/1000 - 1/5000 |
IHC-P
1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. For unpurified use at 1/250 - 1/500 |
Target
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Function
Involved in the transcriptional regulation of multiple genes expressed in the intestinal epithelium. Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine. -
Sequence similarities
Belongs to the Caudal homeobox family.
Contains 1 homeobox DNA-binding domain. -
Post-translational
modificationsPhosphorylation of Ser-60 mediates the transactivation capacity. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 1045 Human
- Entrez Gene: 12591 Mouse
- Entrez Gene: 66019 Rat
- Omim: 600297 Human
- SwissProt: Q99626 Human
- SwissProt: P43241 Mouse
- Unigene: 174249 Human
- Unigene: 20358 Mouse
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Alternative names
- Caudal type homeo box 2 antibody
- Caudal type homeo box transcription factor 2 antibody
- Caudal type homeobox 2 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [EPR2764Y] (ab76541)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labeling CDX2 with Purified ab76541 at 1:1000 dilution (0.8 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Goat Anti-Rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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All lanes : Anti-CDX2 antibody [EPR2764Y] (ab76541) at 1/10000 dilution (Purified)
Lane 1 : Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : Human colon cancer lysates
Lane 3 : Rat colon lysates
Lane 4 : Mouse colon lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/10000 dilution
Predicted band size: 34 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [EPR2764Y] (ab76541)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse colon tissue sections labeling CDX2 with Purified ab76541 at 1:1000 dilution (0.8 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Goat Anti-Rabbit IgG H&L (HRP) ab97051
was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [EPR2764Y] (ab76541)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat colon tissue sections labeling CDX2 with Purified ab76541 at 1:1000 dilution (0.8 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Goat Anti-Rabbit IgG H&L (HRP) ab97051
was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain. -
Lane 1 : Anti-CDX2 antibody [EPR2764Y] (ab76541) at 1/1000 dilution (Unpurified)
Lanes 2-3 : Anti-CDX2 antibody [EPR2764Y] (ab76541) at 1/5000 dilution (Unpurified)
Lane 1 : HT-29 (Human colorectal adenocarcinoma cell line) lysate
Lane 2 : Colon cancer lysate
Lane 3 : Human small intestine lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 34 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [EPR2764Y] (ab76541)This image is courtesy of an anonymous Abreview
Unpurified ab76541 staining CDX2 in human intestinal tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Tissue was fixed with paraformaldehyde and blocked with 5% BSA for 1 hours at 25°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/100 in PBS containing 5% BSA) for 18 hours at 4°C. An HRP-conjugated goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [EPR2764Y] (ab76541)
Unpurified ab76541 showing positive staining in normal colon tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [EPR2764Y] (ab76541)
Unpurified ab76541, at 1/250 dilution, staining CDX2 in paraffin-embedded human gastric adenocarcinoma tissue, by immunohistochemistry. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [EPR2764Y] (ab76541)
Unpurified ab76541 showing positive staining in colonic adenocarcinoma tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [EPR2764Y] (ab76541)
Unpurified ab76541 showing negative staining in skeletal muscle tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [EPR2764Y] (ab76541)
Unpurified ab76541 showing negative staining in normal kidney tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [EPR2764Y] (ab76541)
Unpurified ab76541 showing negative staining in normal brain tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [EPR2764Y] (ab76541)
Unpurified ab76541 showing negative staining in normal brain tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (110)
ab76541 has been referenced in 110 publications.
- Calder MD et al. Effects of palmitic acid on localization of embryo cell fate and blastocyst formation gene products. Reproduction 163:133-143 (2022). PubMed: 35038315
- Saari J et al. Toward Xeno-Free Differentiation of Human Induced Pluripotent Stem Cell-Derived Small Intestinal Epithelial Cells. Int J Mol Sci 23:N/A (2022). PubMed: 35163236
- Shi M et al. Pasteurized Akkermansia muciniphila Ameliorate the LPS-Induced Intestinal Barrier Dysfunction via Modulating AMPK and NF-κB through TLR2 in Caco-2 Cells. Nutrients 14:N/A (2022). PubMed: 35215413
- Engel RM et al. Modeling colorectal cancer: A bio-resource of 50 patient-derived organoid lines. J Gastroenterol Hepatol 37:898-907 (2022). PubMed: 35244298
- Nahaboo W et al. Keratin filaments mediate the expansion of extra-embryonic membranes in the post-gastrulation mouse embryo. EMBO J 41:e108747 (2022). PubMed: 35266581