Anti-CEACAM5 antibody [26/3/13] - BSA and Azide free (ab4451)

Thank you for your email.
Yes, that is correct. Alternatively, you can just use a secondary antibody that has already been conjugated with HRP. We carry many in our catalog. Please let me know if you need help selecting one.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
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The conjugation kit ab102891 is compatible with antibody formulations that contain up to 1% BSA, even though the BSA is in 10-fold excess to the antibody (10mg/ml BSA compared to 1 mg/ml IgG for ab71720). Some of the BSA will itself will be conjugated, affecting efficient conjugation of the IgG, so an antibody without BSA would be better, such as anti-PSMA ab19071.

However, even with 1% BSA, much of the IgG in ab71720 can be conjugated to HRP using the HRP kit ab102891, and will be useful for subsequent assays. I do not know if that is the case for the other company's conjugation kit.

I think the CEA antibody formulation will be compatible with the other company's labeling kit but you should contact them and send them the buffer components. Here are the components of the ab15987 buffer:

Preservative: 15mM Sodium Azide Constituents: 0.1M Sodium chloride, pH 7.2

The concentration of IgG for our current lot of ab15987 is 2.0 mg/ml. There is no protein carrier such as BSA.

The PSMA antibody formulation (ab97080) may be more problematic, as it contains BSA, which may interfere with conjugation of the HRP to the immunoglobulin. The buffer components are:

Preservative: 0.02% Sodium Azide Constituents: 1% BSA, PBS, pH 7.4

The IgG concentration of ab97080 is 1.0 mg/ml.

Again, the other company will be able to tell you if these components are compatible with their conjugation kit. You may need to remove the BSA, in which case I recommend our purification product, ab102784.

https://www.abcam.com/Antibody-Purification-Kit-Protein-A-ab102784.html

We also have an HRP conjugation kit, if you are interested. The catalogue number is ab102891.

https://www.abcam.com/EasyLink-HRP-Conjugation-Kit-3-x-100g-HRP-ab102891.html

I hope this is helpful. If you have any questions, please contact us.

Thank you for your resposne.

As my colleauge has explained in the previous e-mail, our antibodies and proteins will regrettably not have been tested and guaranteed in the application you are using (i.e. electrical biosensors).

As ELISA is the closest application, I am pleased to recommend some antibodies suitable for this applications, however these will not be covered by our guarantee for electrical biosensors.

CA 15.3 - none

TRU-QUANT - none

CA 27.29 - none

CA125 - ab10033, ab10029, ab19551

CEA (carcinoembryonic antigen) - ab15987, ab4451,



If you need any further assistance in the future, please do not hesitate to contact me.

Thank you for your enquiry.

I can confirm that ab4451 and ab15987 have been tested and guarantee for use in sELISA together according to the datasheets. Theab742 Carcino Embryonic Antigen CEA protein is full length native protein so should be suitable as a standard for use with this antibodies.

I hope this information will be helpful to you. If you have any further questions, please do not hesitate to contact us.

For the CEA assay, I recommend the antibodies I mentioned in my previous e-mail, ab4451 for capture and ab15987 for detection, followed by the secondary, anti-rabbit IgG conjugated to HRP, ab97080, and appropriate substrate, for instance, TMB.
For CEA antigen, I recommend ab724:

https://www.abcam.com/Inhibin-alpha-antibody-R1-ab724.html

For the PSMA assay, we do not have a pair of antibodies that has been tested together in sandwich ELISA. However, the following two antibodies are likely to be an appropriate pair, as they are raised against different portions of the PSMA protein: ab19071 for capture, and ab71720 for detection, followed by ab97080, the rabbit IgG-specific HRP conjugate, and substrate.

However, antibody ab71720 has not been tested in ELISA, and I cannot guarantee it will work well in this application, though the western blot looks very good.

We do not have an appropriate PSMA protein in our catalogue, so I suggest searching the website www.biocompare.com.

I hope this helps. If you have any questions, please contact me. For prices, please consult the online datasheets.

Thank you for contacting us. I will be happy to make some suggestions. Can you please confirm that your samples are human-derived, and that you are interested in a sandwich ELISA, rather than direct or indirect ELISA?

For an example of a sandwich ELISA antibody pair that we recommend, two antibodies that have been tested together for detection of human CEA by sandwich ELISA are ab4451 (for capture onto the plate) and ab15987 (for detection). The rabbit polyclonal ab15987 is unconjugated, so you will need to detect it in turn with an anti-rabbit IgG antibody, such as the HRP conjugate ab97080.

For the CEA antigen, and for the PSA assay, we have several choices but I will wait for your information regarding your samples and assay requirements before making suggestions.

Thank you for your inquiry.


You are inquiring about the sELISA pair, ab4451 for capture and ab15987 for detection, correct?


Currently we do not offer a biotinylated form of ab15987. If you are looking to order this antibody in bulk (> 10 vials) we may be able to see if the lab can make a custom preparation. Alternatively, we do sell conjugation kits, which when used with our concentration kit to remove the azide from ab15987, would allow you to directly biotinylate ab15987 in 3 steps.


We sell the conentration kits as 1 column (ab102776) or 3 column (ab102778).


https://www.abcam.com/Antibody-Concentration-Kit-1-column-ab102776.html

https://www.abcam.com/Antibody-Concentration-Kit-3-columns-ab102778.html


We sell the biotinylation kit in different sizes, but I've included the 3 x10ug size below:


https://www.abcam.com/EasyLink-Biotin-type-A-Conjugation-Kit-3-x-10µg-Biotin-ab102865.html


I hope this information helps. Please contact us with any other questions.