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RecombinantRabMAb

Recombinant Anti-CEBP Delta/CEBPD antibody [EPR23518-259] (ab245214)

Submit a review Submit a question References (2)
Western blot - Anti-CEBP Delta/CEBPD antibody [EPR23518-259] (ab245214)
  • Western blot - Anti-CEBP Delta/CEBPD antibody [EPR23518-259] (ab245214)
  • Immunoprecipitation - Anti-CEBP Delta/CEBPD antibody [EPR23518-259] (ab245214)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEBP Delta/CEBPD antibody [EPR23518-259] (ab245214)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEBP Delta/CEBPD antibody [EPR23518-259] (ab245214)
  • Immunohistochemistry (Frozen sections) - Anti-CEBP Delta/CEBPD antibody [EPR23518-259] (ab245214)
  • Immunohistochemistry (Frozen sections) - Anti-CEBP Delta/CEBPD antibody [EPR23518-259] (ab245214)
  • Anti-CEBP Delta/CEBPD antibody [EPR23518-259] (ab245214)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23518-259] to CEBP Delta/CEBPD
  • Suitable for: WB, IHC-P, IHC-Fr, IP
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-CEBP Delta/CEBPD antibody [EPR23518-259]
    See all CEBP Delta/CEBPD primary antibodies

  • Description

    Rabbit monoclonal [EPR23518-259] to CEBP Delta/CEBPD

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IHC-Fr, IPmore details
    Unsuitable for: Flow Cyt or ICC/IF

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: ,Caco-2, HaCaT and differentiated NIH/3T3 L1 cell lysates. IHC-P: Mouse hippocampus tissue; Rat cerebrum tissue. IHC-Fr: Mouse hippocampus tissue; Rat hippocampus tissue. IP: Differentiated NIH/3T3-L1 cells.

  • General notes

    We cannot guarantee ab245214 in IHC-P or IHC-Fr for human samples. Internal testing demonstrated negative staining in positive control human amygdala.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab245214 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
IHC-P
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr
1/100.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
IP
1/30.
Notes
WB
1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
IHC-P
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr
1/100.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
IP
1/30.
Application notes
Is unsuitable for Flow Cyt or ICC/IF.

Target

  • Function

    C/EBP is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers. Important transcriptional activator in the regulation of genes involved in immune and inflammatory responses, may play an important role in the regulation of the several genes associated with activation and/or differentiation of macrophages.

  • Sequence similarities

    Belongs to the bZIP family. C/EBP subfamily.
    Contains 1 bZIP domain.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • C/EBP delta antibody
    • C/EBP related protein 3 antibody
    • CCAAT/enhancer binding protein (C/EBP) delta antibody
    • CCAAT/enhancer binding protein delta antibody
    • CCAAT/enhancer-binding protein delta antibody
    • CEBP D antibody
    • CEBPD antibody
    • CEBPD_HUMAN antibody
    • CELF antibody
    • Crp 3 antibody
    • Crp3 antibody
    • NF IL6 beta antibody
    • NF-IL6-beta antibody
    • Nuclear factor NF IL6 beta antibody
    • Nuclear factor NF-IL6-beta antibody
    see all

Images

  • Western blot - Anti-CEBP Delta/CEBPD antibody [EPR23518-259] (ab245214)
    Western blot - Anti-CEBP Delta/CEBPD antibody [EPR23518-259] (ab245214)
    All lanes : Anti-CEBP Delta/CEBPD antibody [EPR23518-259] (ab245214) at 1/1000 dilution

    Lane 1 : Caco-2 (human colorectal adenocarcinoma epithelial cell), whole cell lysate
    Lane 2 : HaCaT (human skin keratinocyte), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 29 kDa
    Observed band size: 29 kDa


    Exposure time: 180 seconds


    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 180 seconds.

  • Western blot - Anti-CEBP Delta/CEBPD antibody [EPR23518-259] (ab245214)
    Western blot - Anti-CEBP Delta/CEBPD antibody [EPR23518-259] (ab245214)
    Anti-CEBP Delta/CEBPD antibody [EPR23518-259] (ab245214) at 1/1000 dilution + Differentiated 3T3-L1 (mouse embryonic fibroblast), whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 29 kDa
    Observed band size: 29 kDa


    Exposure time: 180 seconds


    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 180 seconds.

  • Immunoprecipitation - Anti-CEBP Delta/CEBPD antibody [EPR23518-259] (ab245214)
    Immunoprecipitation - Anti-CEBP Delta/CEBPD antibody [EPR23518-259] (ab245214)

    CEBP Delta/CEBPD was immunoprecipitated from 0.35 mg differentiated 3T3-L1 (mouse embryonic fibroblast) whole cell lysate with ab245214 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using 245214 1/1000 dilution (0.518 μg/ml). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Differentiated 3T3-L1 (mouse embryonic fibroblast), whole cell lysate 10 μg

    Lane 2: ab245214 IP in Differentiated 3T3-L1 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab245214 in differentiated 3T3-L1 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 180 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEBP Delta/CEBPD antibody [EPR23518-259] (ab245214)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEBP Delta/CEBPD antibody [EPR23518-259] (ab245214)

    Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling CEBP Delta/CEBPD with ab245214 at 1/500 (1.04 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear and weak cytoplasmic staining on rat cerebrum is observed. The section was incubated with ab245214 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEBP Delta/CEBPD antibody [EPR23518-259] (ab245214)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEBP Delta/CEBPD antibody [EPR23518-259] (ab245214)

    Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue labeling CEBP Delta/CEBPD with ab245214 at 1/500 (1.04 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on mouse hippocampus is observed. The section was incubated with ab245214 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

  • Immunohistochemistry (Frozen sections) - Anti-CEBP Delta/CEBPD antibody [EPR23518-259] (ab245214)
    Immunohistochemistry (Frozen sections) - Anti-CEBP Delta/CEBPD antibody [EPR23518-259] (ab245214)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat hippocampus tissue labeling CEBP Delta/CEBPD with ab245214 at 1/100 dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution (Green). The nuclear counterstain was DAPI (Blue). Nuclear staining on rat hippocampus is observed.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution.

  • Immunohistochemistry (Frozen sections) - Anti-CEBP Delta/CEBPD antibody [EPR23518-259] (ab245214)
    Immunohistochemistry (Frozen sections) - Anti-CEBP Delta/CEBPD antibody [EPR23518-259] (ab245214)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse hippocampus tissue labeling CEBP Delta/CEBPD with ab245214 at 1/100 dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution (Green). The nuclear counterstain was DAPI (Blue). Nuclear staining on mouse hippocampus is observed.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution.

  • Anti-CEBP Delta/CEBPD antibody [EPR23518-259] (ab245214)
    Anti-CEBP Delta/CEBPD antibody [EPR23518-259] (ab245214)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (2)

Publishing research using ab245214? Please let us know so that we can cite the reference in this datasheet.

ab245214 has been referenced in 2 publications.

  • Zhang X  et al. FOXO3a regulates lipid accumulation and adipocyte inflammation in adipocytes through autophagy : Role of FOXO3a in obesity. Inflamm Res 70:591-603 (2021). PubMed: 33891136
  • Wang X  et al. In vivo CRISPR screens identify the E3 ligase Cop1 as a modulator of macrophage infiltration and cancer immunotherapy target. Cell 184:5357-5374.e22 (2021). PubMed: 34582788

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