Recombinant Anti-CEBP Delta/CEBPD antibody [EPR23518-259] (ab245214)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23518-259] to CEBP Delta/CEBPD
- Suitable for: WB, IHC-P, IHC-Fr, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-CEBP Delta/CEBPD antibody [EPR23518-259]
See all CEBP Delta/CEBPD primary antibodies -
Description
Rabbit monoclonal [EPR23518-259] to CEBP Delta/CEBPD -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IHC-Fr, IPmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: ,Caco-2, HaCaT and differentiated NIH/3T3 L1 cell lysates. IHC-P: Mouse hippocampus tissue; Rat cerebrum tissue. IHC-Fr: Mouse hippocampus tissue; Rat hippocampus tissue. IP: Differentiated NIH/3T3-L1 cells.
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General notes
We cannot guarantee ab245214 in IHC-P or IHC-Fr for human samples. Internal testing demonstrated negative staining in positive control human amygdala.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23518-259 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab245214 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
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IHC-P |
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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IHC-Fr |
1/100.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
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IP |
1/30.
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Notes |
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WB
1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa). |
IHC-P
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IHC-Fr
1/100. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
IP
1/30. |
Target
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Function
C/EBP is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers. Important transcriptional activator in the regulation of genes involved in immune and inflammatory responses, may play an important role in the regulation of the several genes associated with activation and/or differentiation of macrophages. -
Sequence similarities
Belongs to the bZIP family. C/EBP subfamily.
Contains 1 bZIP domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 1052 Human
- Entrez Gene: 12609 Mouse
- Entrez Gene: 25695 Rat
- Omim: 116898 Human
- SwissProt: P49716 Human
- SwissProt: Q00322 Mouse
- SwissProt: Q03484 Rat
- Unigene: 440829 Human
see all -
Alternative names
- C/EBP delta antibody
- C/EBP related protein 3 antibody
- CCAAT/enhancer binding protein (C/EBP) delta antibody
see all
Images
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All lanes : Anti-CEBP Delta/CEBPD antibody [EPR23518-259] (ab245214) at 1/1000 dilution
Lane 1 : Caco-2 (human colorectal adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : HaCaT (human skin keratinocyte), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa
Exposure time: 180 secondsBlocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 180 seconds.
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Anti-CEBP Delta/CEBPD antibody [EPR23518-259] (ab245214) at 1/1000 dilution + Differentiated 3T3-L1 (mouse embryonic fibroblast), whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa
Exposure time: 180 secondsBlocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 180 seconds.
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CEBP Delta/CEBPD was immunoprecipitated from 0.35 mg differentiated 3T3-L1 (mouse embryonic fibroblast) whole cell lysate with ab245214 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using 245214 1/1000 dilution (0.518 μg/ml). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Differentiated 3T3-L1 (mouse embryonic fibroblast), whole cell lysate 10 μg
Lane 2: ab245214 IP in Differentiated 3T3-L1 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab245214 in differentiated 3T3-L1 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 180 seconds.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEBP Delta/CEBPD antibody [EPR23518-259] (ab245214)
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling CEBP Delta/CEBPD with ab245214 at 1/500 (1.04 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear and weak cytoplasmic staining on rat cerebrum is observed. The section was incubated with ab245214 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEBP Delta/CEBPD antibody [EPR23518-259] (ab245214)
Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue labeling CEBP Delta/CEBPD with ab245214 at 1/500 (1.04 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on mouse hippocampus is observed. The section was incubated with ab245214 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
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Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat hippocampus tissue labeling CEBP Delta/CEBPD with ab245214 at 1/100 dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution (Green). The nuclear counterstain was DAPI (Blue). Nuclear staining on rat hippocampus is observed.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution.
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Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse hippocampus tissue labeling CEBP Delta/CEBPD with ab245214 at 1/100 dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution (Green). The nuclear counterstain was DAPI (Blue). Nuclear staining on mouse hippocampus is observed.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (2)
ab245214 has been referenced in 2 publications.
- Zhang X et al. FOXO3a regulates lipid accumulation and adipocyte inflammation in adipocytes through autophagy : Role of FOXO3a in obesity. Inflamm Res 70:591-603 (2021). PubMed: 33891136
- Wang X et al. In vivo CRISPR screens identify the E3 ligase Cop1 as a modulator of macrophage infiltration and cancer immunotherapy target. Cell 184:5357-5374.e22 (2021). PubMed: 34582788