Name: Anti-CENPC antibody [EPR15939]
Brand: RabMAb
SKU: ab193666
Rating: 4 (3 reviews)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CENPC antibody [EPR15939] (ab193666)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR15939] to CENPC
Suitable for: Flow Cyt (Intra), IP, IHC-P, WB
Reacts with: Mouse, Rat, Human

Carrier Free

Product name

Anti-CENPC antibody [EPR15939]
See all CENPC primary antibodies
Description

Rabbit monoclonal [EPR15939] to CENPC
Host species

Rabbit
Tested applications

Suitable for: Flow Cyt (Intra), IP, IHC-P, WBmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Jurkat, A431, K562 and NIH/3T3 cell lysates. IHC-P: Human spleen tissue; Rat cardiac muscle tissue. Flow Cyt (intra): K562 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR15939
Isotype

IgG
Research areas

Alternative Versions
- Anti-CENPC antibody [EPR15939] - BSA and Azide free (ab232614)
Conjugation kits
- PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
- APC Conjugation Kit - Lightning-Link® (ab201807)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab193666 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
Flow Cyt (Intra)		1/400. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IP		1/100.
IHC-P		1/500 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB		1/1000 - 1/10000. Detects a band of approximately 107 kDa (predicted molecular weight: 107 kDa).

Notes
Flow Cyt (Intra) 1/400. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IP 1/100.
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Detects a band of approximately 107 kDa (predicted molecular weight: 107 kDa).

Function

Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. CENPC recruits DNA methylation and DNMT3B to both centromeric and pericentromeric satellite repeats and regulates the histone code in these regions.
Sequence similarities

Belongs to the CENPC family.
Domain

The MIF2 homology domain II targets centromeres and binds the alpha satellite DNA in vivo. The MIF2 homology domain III can induce CENPC dimerization/oligomerization.
Cellular localization

Nucleus. Chromosome > centromere > kinetochore. Localizes exclusively in the kinetochore domain of centromeres.
Target information above from: UniProt accession Q03188 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 1060 Human
- Entrez Gene: 12617 Mouse
- Entrez Gene: 305270 Rat
- Omim: 117141 Human
- SwissProt: Q03188 Human
- SwissProt: P49452 Mouse
- Unigene: 479867 Human
- Unigene: 4649 Mouse
Alternative names
- CENP-C 1 antibody
- CENP-C antibody
- CENPC_HUMAN antibody
- CENPC1 antibody
- Centromere autoantigen C antibody
- Centromere autoantigen C1 antibody
- Centromere protein C 1 antibody
- Centromere protein C antibody
- hcp-4 antibody
- ICEN7 antibody
- Interphase centromere complex protein 7 antibody
- MIF2 antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CENPC antibody [EPR15939] (ab193666)

Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling CENPC with ab193666 at 1/1,000 dilution, followed by prediluted HRP polymer for Rabbit/Mouse IgG. Counter stained with hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-CENPC antibody [EPR15939] (ab193666)

All lanes : Anti-CENPC antibody [EPR15939] (ab193666) at 1/5000 dilution

Lane 1 : Jurkat cell lysate
Lane 2 : A431 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 107 kDa
Observed band size: 107 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-CENPC antibody [EPR15939] (ab193666)

Anti-CENPC antibody [EPR15939] (ab193666) at 1/10000 dilution + K562 cell lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 107 kDa
Observed band size: 107 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-CENPC antibody [EPR15939] (ab193666)

Anti-CENPC antibody [EPR15939] (ab193666) at 1/1000 dilution + NIH/3T3 cell lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 107 kDa
Observed band size: 107 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CENPC antibody [EPR15939] (ab193666)

Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labeling CENPC with ab193666 at 1/1000 dilution (2μg/ml), followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cytometry (Intracellular) - Anti-CENPC antibody [EPR15939] (ab193666)

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed K562 cells labeling CENPC with ab193666 at 1/400 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-CENPC antibody [EPR15939] (ab193666)

Western blot analysis of CENPC immunoprecipitated from Jurkat cell lysate using ab193666 at 1/100 dilution.
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.
Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-CENPC antibody [EPR15939] (ab193666)

Flow cytometry protocols
Immunoprecipitation protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab193666? Please let us know so that we can cite the reference in this datasheet.

ab193666 has been referenced in 3 publications.

Fan J et al. Immunization with CENP-C Causes Aberrant Chromosome Segregation during Oocyte Meiosis in Mice. J Immunol Res 2021:4610494 (2021). PubMed: 33604391
Sales-Gil R et al. Non-redundant functions of H2A.Z.1 and H2A.Z.2 in chromosome segregation and cell cycle progression. EMBO Rep 22:e52061 (2021). PubMed: 34423893
Beh TT et al. Active centromere and chromosome identification in fixed cell lines. Mol Cytogenet 9:28 (2016). ICC/IF ; Human . PubMed: 27011768

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Application

Immunocytochemistry/ Immunofluorescence

Sample

Rat Cell (Bone marrow smear)

Permeabilization

Specification

Bone marrow smear

Fixative

Methanol

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 25 2019

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (HeLa)

Permeabilization

Specification

HeLa

Fixative

Methanol

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Kirk Mcmanus

Verified customer

Submitted Aug 25 2017

Application

ChIP

Sample

Symphalangus syndactylus (Siamang Gibbon) Cell lysate - nuclear (EBV transformed gibbon lymphocytes)

Negative control

IgG

Specification

EBV transformed gibbon lymphocytes

Detection step

Other

Type

Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jul 19 2017

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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Conjugation kits

Isotype control

Positive Controls

Applications

The Abpromise guarantee

Target

Function

Sequence similarities

Domain

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

Certificate of Compliance

References (3)

Customer reviews and Q&As

Immunocytochemistry/ Immunofluorescence abreview for Anti-CENPC antibody [EPR15939]

Immunocytochemistry/ Immunofluorescence abreview for Anti-CENPC antibody [EPR15939]

ChIP abreview for Anti-CENPC antibody [EPR15939]