Recombinant Anti-CENPE antibody [EPR4542(2)] (ab133583)
![Western blot - Anti-CENPE antibody [EPR4542(2)] (ab133583)](https://www.abcam.com/ps/products/133/ab133583/Images/ab133583-256325-anti-cenpe-antibody-epr45422-western-blot.jpg "Western blot - Anti-CENPE antibody [EPR4542(2)] (ab133583)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4542(2)] to CENPE
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CENPE antibody [EPR4542(2)]
See all CENPE primary antibodies -
Description
Rabbit monoclonal [EPR4542(2)] to CENPE -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa treated with aphidicolin and Nocodazole, HepG2 and Jurkat cell lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 3.30 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4542(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133583 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 312 kDa.
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| Notes |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 312 kDa. |
Target
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Function
Essential for the maintenance of chromosomal stability through efficient stabilization of microtubule capture at kinetochores. Plays a key role in the movement of chromosomes toward the metaphase plate during mitosis. Is a slow plus end-directed motor whose activity is essential for metaphase chromosome alignment. Couples chromosome position to microtubule depolymerizing activity. The highly processive microtubule-dependent motor activity of CENPE serves to power chromosome congression and provides a flexible, motile tether linking kinetochores to dynamic spindle microtubules. Necessary for the mitotic checkpoint signal at individual kinetochores to prevent aneuploidy due to single chromosome loss. Required for the efficient recruitment of BUBR1, MAD1 and MAD2 to attached and newly unattached kinetochores. Stimulates mammalian BUBR1 kinase activity. Accumulates just before mitosis at the G2 phase of the cell cycle. -
Involvement in disease
Microcephaly 13, primary, autosomal recessive -
Sequence similarities
Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.
Contains 1 kinesin motor domain. -
Domain
The protein is composed of a N-terminal kinesin-motor domain involved in the chromosome movements, a long coil-coiled region involved in the homodimerization and an inhibitory C-tail involved in autoinhibition of the N-terminal catalytic part. -
Post-translational
modificationsThe C-terminal inhibitory domain is phosphorylated. Phosphorylation relieves autoinhibition of the kinetochore motor.
Sumoylated with SUMO2 and SUMO3. The sumoylation mediates the association to the kinetochore. -
Cellular localization
Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle. Associates with kinetochores during congression (as early as prometaphase), relocates to the spindle midzone at anaphase, and is quantitatively discarded at the end of the cell division. - Information by UniProt
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Database links
- Entrez Gene: 1062 Human
- Omim: 117143 Human
- SwissProt: Q02224 Human
- Unigene: 75573 Human
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Alternative names
- CENP E antibody
- CENP-E antibody
- CENPE antibody
see all
Images
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Western blot - Anti-CENPE antibody [EPR4542(2)] (ab133583)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: CENPE knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: HepG2 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab133583 observed at 310 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab133583 was shown to specifically react with CENPE when CENPE knockout samples were used. Wild-type and CENPE knockout samples were subjected to SDS-PAGE. ab133583 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging. -
![Western blot - Anti-CENPE antibody [EPR4542(2)] (ab133583)](https://www.abcam.com/ps/products/133/ab133583/Images/ab133583-147384-anti-cenpe-antibody-epr45422-western-blot.jpg)
Western blot - Anti-CENPE antibody [EPR4542(2)] (ab133583)All lanes : Anti-CENPE antibody [EPR4542(2)] (ab133583) at 1/1000 dilution
Lane 1 : HeLa treated with aphidicolin and Nocodazole cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution
Predicted band size: 312 kDa -
![OI-RD Scanning - Anti-CENPE antibody [EPR4542(2)] (ab133583)](https://www.abcam.com/ps/products/133/ab133583/Images/ab133583-147383-anti-cenpe-antibody-epr45422.jpg)
OI-RD Scanning - Anti-CENPE antibody [EPR4542(2)] (ab133583) -
![Anti-CENPE antibody [EPR4542(2)] (ab133583)](https://www.abcam.com/ps/products/133/ab133583/Images/ab133583-5-benefits-of-recombinant-antibodies.png)
Anti-CENPE antibody [EPR4542(2)] (ab133583)
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab133583 has been referenced in 9 publications.
- Almeida AC et al. Augmin-dependent microtubule self-organization drives kinetochore fiber maturation in mammals. Cell Rep 39:110610 (2022). PubMed: 35385739
- Gomes AM et al. Micronuclei from misaligned chromosomes that satisfy the spindle assembly checkpoint in cancer cells. Curr Biol 32:4240-4254.e5 (2022). PubMed: 36057259
- Yu KW et al. Kinesin-7 CENP-E regulates cell division, gastrulation and organogenesis in development. Eur J Cell Biol 99:151107 (2020). PubMed: 32800279
- Steblyanko Y et al. Microtubule poleward flux in human cells is driven by the coordinated action of four kinesins. EMBO J 39:e105432 (2020). PubMed: 33073400
- Ma C et al. CENPE promotes glioblastomas proliferation by directly binding to WEE1. Transl Cancer Res 9:717-725 (2020). PubMed: 35117417
- Tan Z et al. Environmental stresses induce karyotypic instability in colorectal cancer cells. Mol Biol Cell 30:42-55 (2019). PubMed: 30379607
- Shan L et al. CENPE promotes lung adenocarcinoma proliferation and is directly regulated by FOXM1. Int J Oncol 55:257-266 (2019). PubMed: 31115500
- Liang Y et al. LSD1-Mediated Epigenetic Reprogramming Drives CENPE Expression and Prostate Cancer Progression. Cancer Res 77:5479-5490 (2017). PubMed: 28916652
- Beh TT et al. Active centromere and chromosome identification in fixed cell lines. Mol Cytogenet 9:28 (2016). ICC/IF ; Human . PubMed: 27011768