Anti-Ceruloplasmin antibody (ab48614)
Key features and details
- Rabbit polyclonal to Ceruloplasmin
- Suitable for: IHC-P, IP, RIA, EIA, ELISA, ICC/IF, WB, IHC-FoFr
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Ceruloplasmin antibody
See all Ceruloplasmin primary antibodies -
Description
Rabbit polyclonal to Ceruloplasmin -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, IP, RIA, EIA, ELISA, ICC/IF, WB, IHC-FoFrmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Human ceruloplasmin purified from human plasma
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.50
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 50% Glycerol -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab48614 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| IP |
Use at an assay dependent concentration.
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| RIA |
Use at an assay dependent concentration.
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| EIA |
Use at an assay dependent concentration.
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| ELISA |
Use at an assay dependent concentration.
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| ICC/IF |
Use at an assay dependent concentration.
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| WB | (1) |
Use at an assay dependent concentration. Predicted molecular weight: 122 kDa.
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| IHC-FoFr | (1) |
Use at an assay dependent concentration.
|
| Notes |
|---|
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IHC-P
Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
|
IP
Use at an assay dependent concentration. |
|
RIA
Use at an assay dependent concentration. |
|
EIA
Use at an assay dependent concentration. |
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ELISA
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 122 kDa. |
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IHC-FoFr
Use at an assay dependent concentration. |
Target
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Function
Ceruloplasmin is a blue, copper-binding (6-7 atoms per molecule) glycoprotein. It has ferroxidase activity oxidizing Fe(2+) to Fe(3+) without releasing radical oxygen species. It is involved in iron transport across the cell membrane. -
Tissue specificity
Expressed by the liver and secreted in plasma. -
Involvement in disease
Defects in CP are the cause of aceruloplasminemia (ACERULOP) [MIM:604290]. It is an autosomal recessive disorder of iron metabolism characterized by iron accumulation in the brain as well as visceral organs. Clinical features consist of the triad of retinal degeneration, diabetes mellitus and neurological disturbances.
Note=Ceruloplasmin levels are decreased in Wilson disease, in which copper cannot be incorporated into ceruloplasmin in liver because of defects in the copper-transporting ATPase 2. -
Sequence similarities
Belongs to the multicopper oxidase family.
Contains 3 F5/8 type A domains.
Contains 6 plastocyanin-like domains. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 1356 Human
- Entrez Gene: 12870 Mouse
- Omim: 117700 Human
- SwissProt: P00450 Human
- SwissProt: Q61147 Mouse
- Unigene: 558314 Human
- Unigene: 13787 Mouse
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Alternative names
- CERU_HUMAN antibody
- Ceruloplasmin antibody
- CP 2 antibody
see all
Images
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Western blot - Anti-Ceruloplasmin antibody (ab48614)Anti-Ceruloplasmin antibody (ab48614) at 1 µg/ml + Human Plasma Total Protein Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 122 kDa
Observed band size: 122,148 kDa why is the actual band size different from the predicted?
Additional bands at: 34 kDa, 76 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
Ceruloplasmin contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted (148 kDa). -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ceruloplasmin antibody (ab48614)Ab48614 staining human tonsil. Staining is localized to the cytoplasm.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system (Dako PT Link), at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer, EDTA pH 9.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (12)
ab48614 has been referenced in 12 publications.
- Shin HJ et al. Lipocalin-2 Deficiency Reduces Oxidative Stress and Neuroinflammation and Results in Attenuation of Kainic Acid-Induced Hippocampal Cell Death. Antioxidants (Basel) 10:N/A (2021). PubMed: 33445746
- Madani AY et al. Signal Transducer and Activator of Transcription 3 (STAT3) Suppresses STAT1/Interferon Signaling Pathway and Inflammation in Senescent Preadipocytes. Antioxidants (Basel) 10:N/A (2021). PubMed: 33672392
- Tsai YM et al. Loss of miR-145-5p Causes Ceruloplasmin Interference with PHD-Iron Axis and HIF-2a Stabilization in Lung Adenocarcinoma-Mediated Angiogenesis. Int J Mol Sci 21:N/A (2020). PubMed: 32708433
- Ma X et al. Identification of a distinct luminal subgroup diagnosing and stratifying early stage prostate cancer by tissue-based single-cell RNA sequencing. Mol Cancer 19:147 (2020). PubMed: 33032611
- Song Z et al. Comprehensive Proteomic Profiling of Urinary Exosomes and Identification of Potential Non-invasive Early Biomarkers of Alzheimer's Disease in 5XFAD Mouse Model. Front Genet 11:565479 (2020). PubMed: 33250918