Recombinant Anti-CFHR5 antibody [EPR25711-28] (ab305247)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR25711-28] to CFHR5
- Suitable for: WB, Dot blot
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CFHR5 antibody [EPR25711-28]
See all CFHR5 primary antibodies -
Description
Rabbit monoclonal [EPR25711-28] to CFHR5 -
Host species
Rabbit -
Tested applications
Suitable for: WB, Dot blotmore details
Unsuitable for: Flow Cyt (Intra),ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Untreated human plasma and human plasma treated with Protein Deglycosylation MIX II. Human liver and kidney tissue lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR25711-28 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab305247 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 64 kDa).
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Dot blot |
1/1000.
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Notes |
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WB
1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 64 kDa). |
Dot blot
1/1000. |
Target
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Function
Involved in complement regulation. -
Tissue specificity
Expressed by the liver and secreted in plasma. -
Involvement in disease
Note=Defects in CFHR5 have been found in patients with atypical hemolytic uremic syndrome and may contribute to the disease. Atypical hemolytic uremic syndrome is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype. -
Sequence similarities
Contains 9 Sushi (CCP/SCR) domains. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 81494 Human
- Omim: 608593 Human
- SwissProt: Q9BXR6 Human
- Unigene: 282594 Human
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Alternative names
- CFHL5 antibody
- CFHR5 antibody
- CFHR5D antibody
see all
Images
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All lanes : Anti-CFHR5 antibody [EPR25711-28] (ab305247) at 1/1000 dilution
Lane 1 : Human liver tissue lysate
Lane 2 : Human kidney tissue lysate
Lane 3 : Human heart tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 64 kDa
Additional bands at: 70 kDa (possible glycosylated form)Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: human heart (Human Integrated Protein Expression Database-GeneCards).
Exposure time: 59 seconds.
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All lanes : Anti-CFHR5 antibody [EPR25711-28] (ab305247) at 1/1000 dilution
Lane 1 : Untreated human plasma
Lane 2 : Human plasma treated with Protein Deglycosylation MIX II
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 64 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 180 seconds.
Observed MW (kDa): 64 (de-glycosylated form), 70 (glycosylated form).
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Dot blot analysis of CFHR5 using AB305247 at 1:1000 (0.507 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1:100,000 dilution.
Exposure time: 103 seconds.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Lane 1: His-tagged human CFHR1 recombinant fragment 10 ng
Lane 2: His-tagged human CFHR2 recombinant fragment 10 ng
Lane 3: His-tagged human CFHR3 recombinant fragment 10 ng
Lane 4: His-tagged human CFHR4 recombinant fragment 10 ng
Lane 5: His-tagged human CFHR5 recombinant fragment 10 ngThis antibody does not cross-react with human CFHR1, CFHR2, CFHR3 or CFHR4.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab305247 has not yet been referenced specifically in any publications.