Anti-CGRP antibody (ab47027)
Key features and details
- Rabbit polyclonal to CGRP
- Suitable for: IHC-P
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-CGRP antibody
See all CGRP primary antibodies -
Description
Rabbit polyclonal to CGRP -
Host species
Rabbit -
Specificity
The immunogen used to raise this antibody has 78% homology with the human CGRP protein. Previous batches of ab47027 have shown to work in human tissue. Please contact Abcam Scientific Support for more information. -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Mouse
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab47101) -
Positive control
- IHC-P: FFPE rat dorsal root ganglion, spinal cord and rat thyroid tissue sections.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab47027 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (4) |
Use a concentration of 0.1 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
|---|
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IHC-P
Use a concentration of 0.1 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Relevance
CGRP is present in C-cells of the thyroid and in central and peripheral nerves. CGRP has several important physiologic roles: it is a potent vasodilator, and can affect the force and rate of heart beat; it can modulate acetylcholine receptor function at the neuromuscular junction; it has been demonstrated to block tolerance to morphine; and it can modulate antigen presentation in Langerhans cells in the skin. -
Cellular localization
Endoplasmic reticulum and Secreted -
Database links
- Entrez Gene: 116903 Mouse
- Entrez Gene: 12310 Mouse
- Entrez Gene: 171519 Rat
- Entrez Gene: 24241 Rat
- SwissProt: P70160 Mouse
- SwissProt: Q99JA0 Mouse
- SwissProt: P01256 Rat
- SwissProt: P01257 Rat
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Alternative names
- Calcitonin/calcitonin related polypeptide alpha antibody
- Alpha type CGRP antibody
- Beta type CGRP antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CGRP antibody (ab47027)
IHC image of CGRP staining in a Rat normal thyroid formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab47027, 0.1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CGRP antibody (ab47027)IHC image of CGRP staining in rat dorsal root ganglion formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab47027, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CGRP antibody (ab47027)This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United KingdomIHC-P image of CGRP staining on Rat spinal cord sections using ab47027 (1:400). The sections were deparafinized and subjected to heat mediated antigen retrieval. The sections were then blocked using 1% BSA at 21°C for 10 min. The primary antibody was used 21°C for 2 hours.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (27)
ab47027 has been referenced in 27 publications.
- Liu TT et al. Efficacy profile of noninvasive vagus nerve stimulation on cortical spreading depression susceptibility and the tissue response in a rat model. J Headache Pain 23:12 (2022). PubMed: 35062860
- Zhu WY et al. Biodegradable magnesium implant enhances angiogenesis and alleviates medication-related osteonecrosis of the jaw in rats. J Orthop Translat 33:153-161 (2022). PubMed: 35415073
- Wang L et al. Overexpression of BDNF in the ventrolateral periaqueductal gray regulates the behavior of epilepsy-migraine comorbid rats. Brain Behav 12:e2594 (2022). PubMed: 35557046
- Zhang J et al. Epigenetic restoration of voltage-gated potassium channel Kv1.2 alleviates nerve injury-induced neuropathic pain. J Neurochem 156:367-378 (2021). PubMed: 32621322
- Matsuda H et al. Comparison of CGRP distributions in the maxillary sinus and trigeminal ganglion between elderly dentulous and edentulous humans. Eur J Histochem 65:N/A (2021). PubMed: 33787056