Recombinant Anti-CHM antibody [EPR7142] (ab134964)
![Western blot - Anti-CHM antibody [EPR7142] (ab134964)](https://www.abcam.com/ps/products/134/ab134964/Images/ab134964-149202-anti-chm-antibody-epr7142-western-blot.jpg "Western blot - Anti-CHM antibody [EPR7142] (ab134964)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7142] to CHM
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CHM antibody [EPR7142]
See all CHM primary antibodies -
Description
Rabbit monoclonal [EPR7142] to CHM -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, Y79, A431, and 293T cell lysates. A431 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR7142 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134964 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 95 kDa (predicted molecular weight: 73 kDa).
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| ICC/IF |
1/250 - 1/500.
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 95 kDa (predicted molecular weight: 73 kDa). |
|
ICC/IF
1/250 - 1/500. |
Application notes
Is unsuitable for IHC-P or IP.
Target
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Function
Binds unprenylated Rab proteins, presents it to the catalytic Rab GGTase dimer, and remains bound to it after the geranylgeranyl transfer reaction. The component A is thought to be regenerated by transferring its prenylated Rab back to the donor membrane. Also a pre-formed complex consisting of CHM and the Rab GGTase dimer (RGGT or component B) can bind to and prenylate Rab proteins; this alternative pathway is proposed to be the predominant pathway for Rab protein geranylgeranylation. -
Involvement in disease
Defects in CHM are the cause of choroideremia (CHM) [MIM:303100]. An X-linked recessive disease characterized by a slowly progressive degeneration of the choroid, photoreceptors, and retinal pigment epithelium. Affected males develop night blindness in their teenage years followed by loss of peripheral vision and complete blindness at middle age. Carrier females are generally asymptomatic but funduscopic examination often shows patchy areas of chorioretinal atrophy. -
Sequence similarities
Belongs to the Rab GDI family. - Information by UniProt
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Database links
- Entrez Gene: 1121 Human
- Entrez Gene: 12662 Mouse
- Entrez Gene: 24942 Rat
- Omim: 300390 Human
- SwissProt: P24386 Human
- SwissProt: Q9QXG2 Mouse
- SwissProt: P37727 Rat
- Unigene: 496449 Human
see all -
Alternative names
- CHM antibody
- Chm antibody
- Choroideraemia protein antibody
see all
Images
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Western blot - Anti-CHM antibody [EPR7142] (ab134964)All lanes : Anti-CHM antibody [EPR7142] (ab134964) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Y79 cell lysate
Lane 3 : A431 cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 73 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted? -
![Immunocytochemistry/ Immunofluorescence - Anti-CHM antibody [EPR7142] (ab134964)](https://www.abcam.com/ps/products/134/ab134964/Images/ab134964-149201-anti-chm-antibody-epr7142-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CHM antibody [EPR7142] (ab134964)Immunofluorescence analysis of CHM in A431 cells, using ab134964 at a dilution of 1/250. -
![Anti-CHM antibody [EPR7142] (ab134964)](https://www.abcam.com/ps/products/134/ab134964/Images/ab134964-3-benefits-of-recombinant-antibodies.png)
Anti-CHM antibody [EPR7142] (ab134964)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab134964 has been referenced in 2 publications.
- Qi Y et al. RhoA/ROCK Pathway Activation is Regulated by AT1 Receptor and Participates in Smooth Muscle Migration and Dedifferentiation via Promoting Actin Cytoskeleton Polymerization. Int J Mol Sci 21:N/A (2020). PubMed: 32751352
- Moissoglu K et al. RNA localization and co-translational interactions control RAB13 GTPase function and cell migration. EMBO J 39:e104958 (2020). PubMed: 32946136