Anti-Cholecystokinin antibody (ab27441)
Key features and details
- Rabbit polyclonal to Cholecystokinin
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Cholecystokinin antibody
See all Cholecystokinin primary antibodies -
Description
Rabbit polyclonal to Cholecystokinin -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Cholecystokinin aa 50 to the C-terminus (N terminal). The exact sequence is proprietary.
Database link: P06307 -
Positive control
- Stomach. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: CaCO2
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab27441 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/200. Do not perform antigen retrieval. Incubate for 10 minutes at room temperature.
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| ICC/IF |
Use a concentration of 10 µg/ml.
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| WB |
Use at an assay dependent concentration. Predicted molecular weight: 12 kDa.
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| Notes |
|---|
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IHC-P
1/200. Do not perform antigen retrieval. Incubate for 10 minutes at room temperature. |
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ICC/IF
Use a concentration of 10 µg/ml. |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 12 kDa. |
Target
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Function
This peptide hormone induces gall bladder contraction and the release of pancreatic enzymes in the gut. Its function in the brain is not clear. Binding to CCK-A receptors stimulates amylase release from the pancreas, binding to CCK-B receptors stimulates gastric acid secretion. -
Sequence similarities
Belongs to the gastrin/cholecystokinin family. -
Post-translational
modificationsThe precursor is cleaved by proteases to produce a number of active cholecystokinins. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 885 Human
- Omim: 118440 Human
- SwissProt: P06307 Human
- Unigene: 458426 Human
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Alternative names
- (1-49)-CCK58 antibody
- CCK antibody
- CCK12 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Cholecystokinin antibody (ab27441)
ICC/IF image of ab27441 stained CaCO2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab27441 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cholecystokinin antibody (ab27441)Ab27441, at a dilution of 1/200, staining Cholecystokinin in formalin fixed, paraffin embedded stomach tissue by Immunohistochemistry.
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Western blot - Anti-Cholecystokinin antibody (ab27441)Anti-Cholecystokinin antibody (ab27441) at 1/25 dilution + Brain cell lysate
Predicted band size: 12 kDa
Observed band size: 12 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab27441 has been referenced in 6 publications.
- Funakoshi A et al. Cholecystokinin Receptor Antagonist Suppresses Melanoma Growth by Inducing Apoptosis of Tumor Cells. JID Innov 2:100153 (2022). PubMed: 36262666
- Calo J et al. First evidence for the presence of amino acid sensing mechanisms in the fish gastrointestinal tract. Sci Rep 11:4933 (2021). PubMed: 33654150
- Barrios CE et al. Localization and distribution of CCK-8, NPY, Leu-ENK-, and Ghrelin- in the digestive tract of Prochilodus lineatus (Valenciennes, 1836). An Acad Bras Cienc 92:e20181165 (2020). PubMed: 32556047
- Funakoshi A et al. Secukinumab promotes engagement of cholecystokinin and its receptor in epidermal keratinocytes of psoriasis patients. J Dermatol 47:1454-1456 (2020). PubMed: 32794215
- Funakoshi A et al. Cholecystokinin Downregulates Psoriatic Inflammation by Its Possible Self-Regulatory Effect on Epidermal Keratinocytes. J Immunol 202:2609-2615 (2019). PubMed: 30902899
- Ye DZ & Kaestner KH Foxa1 and Foxa2 control the differentiation of goblet and enteroendocrine L- and D-cells in mice. Gastroenterology 137:2052-62 (2009). IHC-P ; Mouse . PubMed: 19737569