Recombinant Anti-Cip4 antibody [EPR1965] (ab108313)
![Western blot - Anti-Cip4 antibody [EPR1965] (ab108313)](https://www.abcam.com/ps/products/108/ab108313/Images/ab108313-410135-anti-cip4-antibody-epr1965-western-blot-human-knockout-lysate.jpg "Western blot - Anti-Cip4 antibody [EPR1965] (ab108313)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1965] to Cip4
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Cip4 antibody [EPR1965]
See all Cip4 primary antibodies -
Description
Rabbit monoclonal [EPR1965] to Cip4 -
Host species
Rabbit -
Specificity
Recent WB re-tests performed by our lab suggest to use a higher dilution for mouse samples. The tested dilutions were 1:500 and 1:2000, were the signal detected was very strong. Human and rat samples worked fine in these dilutions. -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK293T, JAR, HepG2, TF1, and HeLa cell lysates. IHC-P: Human stomach tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR1965 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab108313 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 68 kDa.
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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| Notes |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 68 kDa. |
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IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
Target
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Function
Required for translocation of GLUT4 to the plasma membrane in response to insulin signaling (By similarity). Required to coordinate membrane tubulation with reorganization of the actin cytoskeleton during endocytosis. Binds to lipids such as phosphatidylinositol 4,5-bisphosphate and phosphatidylserine and promotes membrane invagination and the formation of tubules. Also promotes CDC42-induced actin polymerization by recruiting WASL/N-WASP which in turn activates the Arp2/3 complex. Actin polymerization may promote the fission of membrane tubules to form endocytic vesicles. Required for the formation of podosomes, actin-rich adhesion structures specific to monocyte-derived cells. May be required for the lysosomal retention of FASLG/FASL. -
Tissue specificity
Expressed in brain, colon, heart, kidney, liver, lung, megakaryocyte, ovary, pancreas, peripheral blood lymphocytes, placenta, prostate, skeletal muscle, small intestine, spleen, testis, thymus and trachea. -
Sequence similarities
Belongs to the FNBP1 family.
Contains 1 FCH domain.
Contains 1 REM (Hr1) repeat.
Contains 1 SH3 domain. -
Post-translational
modificationsTyrosine phosphorylated. Also phosphorylated by PKA. -
Cellular localization
Cytoplasm > perinuclear region and Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. Lysosome. Golgi apparatus. Cell membrane. Cell projection > phagocytic cup. Translocates to the plasma membrane in response to insulin stimulation, and this may require active RHOQ (By similarity). Localizes to cortical regions coincident with F-actin, to lysosomes and to sites of phagocytosis in macrophages. Also localizes to the Golgi, and this requires AKAP9. - Information by UniProt
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Database links
- Entrez Gene: 9322 Human
- Entrez Gene: 106628 Mouse
- Entrez Gene: 116717 Rat
- Omim: 604504 Human
- SwissProt: Q15642 Human
- SwissProt: Q8CJ53 Mouse
- SwissProt: P97531 Rat
- Unigene: 515094 Human
see all -
Alternative names
- Cdc42 interacting protein 4 antibody
- Cdc42 interaction protein 4 long isoform antibody
- Cdc42-interacting protein 4 antibody
see all
Images
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Western blot - Anti-Cip4 antibody [EPR1965] (ab108313)All lanes : Anti-Cip4 antibody [EPR1965] (ab108313) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : TRIP10 knockout HEK293T cell lysate
Lane 3 : JAR cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 68 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab108313 observed at 75 kDa. Red - loading control ab8245 observed at 36 kDa.
ab108313 Anti-Cip4 antibody [EPR1965] was shown to specifically react with Cip4 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266428 (knockout cell lysate ab258251) was used. Wild-type and Cip4 knockout samples were subjected to SDS-PAGE. ab108313 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cip4 antibody [EPR1965] (ab108313)](https://www.abcam.com/ps/products/108/ab108313/Images/ab108313-120867-anti-cip4-antibody-epr1965-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cip4 antibody [EPR1965] (ab108313)ab108313 at 1/100 dilution staining Cip4 in paraffin embedded Human stomach tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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![Western blot - Anti-Cip4 antibody [EPR1965] (ab108313)](https://www.abcam.com/ps/products/108/ab108313/Images/ab108313-120868-anti-cip4-antibody-epr1965-western-blot.jpg)
Western blot - Anti-Cip4 antibody [EPR1965] (ab108313)All lanes : Anti-Cip4 antibody [EPR1965] (ab108313) at 1/1000 dilution
Lane 1 : JAR cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : TF1 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 68 kDa -
![Anti-Cip4 antibody [EPR1965] (ab108313)](https://www.abcam.com/ps/products/108/ab108313/Images/ab108313-5-benefits-of-recombinant-antibodies.png)
Anti-Cip4 antibody [EPR1965] (ab108313)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab108313 has been referenced in 1 publication.
- Meng DF et al. CDC42-interacting protein 4 promotes metastasis of nasopharyngeal carcinoma by mediating invadopodia formation and activating EGFR signaling. J Exp Clin Cancer Res 36:21 (2017). PubMed: 28129778