Recombinant Anti-Clathrin heavy chain antibody [EPR12235(B)] (ab172958)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clathrin heavy chain antibody [EPR12235(B)] (ab172958)](https://www.abcam.com/ps/products/172/ab172958/Images/ab172958-196127-anti-clathrin-heavy-chain-antibody-epr12235b-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clathrin heavy chain antibody [EPR12235(B)] (ab172958)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12235(B)] to Clathrin heavy chain
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-Clathrin heavy chain antibody [EPR12235(B)]
See all Clathrin heavy chain primary antibodies -
Description
Rabbit monoclonal [EPR12235(B)] to Clathrin heavy chain -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow
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Immunogen
Synthetic peptide within Human Clathrin heavy chain aa 200-300 (Cysteine residue). The exact sequence is proprietary.
Database link: Q00610 -
Positive control
- Hela, SH-SY5Y, A431, NIH/3T3, 293T cell lysates, Human colonic carcinoma tissue, Human kidney tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12235(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab172958 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/10000 - 1/50000. Predicted molecular weight: 191 kDa.
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| ICC/IF |
1/250.
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| Notes |
|---|
|
WB
1/10000 - 1/50000. Predicted molecular weight: 191 kDa. |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
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ICC/IF
1/250. |
Target
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Function
Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network. -
Sequence similarities
Belongs to the clathrin heavy chain family. -
Cellular localization
Cytoplasmic vesicle membrane. Membrane > coated pit. Melanosome. Cytoplasmic face of coated pits and vesicles. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 281080 Cow
- Entrez Gene: 1213 Human
- Entrez Gene: 67300 Mouse
- Entrez Gene: 54241 Rat
- Omim: 118955 Human
- SwissProt: P49951 Cow
- SwissProt: P53675 Human
- SwissProt: Q00610 Human
see all -
Alternative names
- CHC17 antibody
- Clathrin heavy chain antibody
- Clathrin heavy chain 1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clathrin heavy chain antibody [EPR12235(B)] (ab172958)
Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Clathrin heavy chain with ab172958 at 1/50 dilution
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-Clathrin heavy chain antibody [EPR12235(B)] (ab172958)](https://www.abcam.com/ps/products/172/ab172958/Images/ab172958-387113-anti-clathrin-heavy-chain-antibody-epr12235-30-immunocytochemistry-a431-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Clathrin heavy chain antibody [EPR12235(B)] (ab172958)Immunocytochemistry/ Immunofluorescence analysis of A431 (human epidermoid carcinoma epithelial cell) labeling Clathrin heavy chain with ab172958 at 1/250 (4.1 μg/mL). ab150077, AlexaFluor®488 Goat anti-Rabbit at 1/1000 (2 μg/mL) was used as the secondary antibody. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% TritonX-100. ab195889, Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 μg/mL) was used as counterstain antibody. Nuclei were stained blue with DAPI.Confocal image showing cytoplasmic staining in A431 cells. -
![Immunocytochemistry/ Immunofluorescence - Anti-Clathrin heavy chain antibody [EPR12235(B)] (ab172958)](https://www.abcam.com/ps/products/172/ab172958/Images/ab172958-387114-anti-clathrin-heavy-chain-antibody-epr12235-30-immunocytochemistry-nih-3t3-mouse.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Clathrin heavy chain antibody [EPR12235(B)] (ab172958)Immunocytochemistry/ Immunofluorescence analysis of NIH/3T3 (mouse embryonic fibroblast) labeling Clathrin heavy chain with ab172958 at 1/250 (4.1 μg/mL). ab150077, AlexaFluor®488 Goat anti-Rabbit at 1/1000 (2 μg/mL) was used as the secondary antibody. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% TritonX-100. ab195889, Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 μg/mL) was used as counterstain antibody. Nuclei were stained blue with DAPI.
Confocal image showing cytoplasmic staining in NIH/3T3 cells.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clathrin heavy chain antibody [EPR12235(B)] (ab172958)](https://www.abcam.com/ps/products/172/ab172958/Images/ab172958-196125-anti-clathrin-heavy-chain-antibody-epr12235b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clathrin heavy chain antibody [EPR12235(B)] (ab172958)Immunohistochemical analysis of paraffin-embedded Human colonic carcinoma tissue labeling Clathrin heavy chain with ab172958 at 1/50 dilution
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Western blot - Anti-Clathrin heavy chain antibody [EPR12235(B)] (ab172958)](https://www.abcam.com/ps/products/172/ab172958/Images/ab172958-196124-anti-clathrin-heavy-chain-antibody-epr12235b-western-blot.jpg)
Western blot - Anti-Clathrin heavy chain antibody [EPR12235(B)] (ab172958)All lanes : Anti-Clathrin heavy chain antibody [EPR12235(B)] (ab172958) at 1/10000 dilution
Lane 1 : Hela cell lysate
Lane 2 : SH-SY5Y cell lysate
Lane 3 : A431 cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit at 1/2000 dilution
Predicted band size: 191 kDa -
![Anti-Clathrin heavy chain antibody [EPR12235(B)] (ab172958)](https://www.abcam.com/ps/products/172/ab172958/Images/ab172958-6-benefits-of-recombinant-antibodies.png)
Anti-Clathrin heavy chain antibody [EPR12235(B)] (ab172958)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab172958 has been referenced in 5 publications.
- Sui X et al. METTL3-mediated m6A is required for murine oocyte maturation and maternal-to-zygotic transition. Cell Cycle 19:391-404 (2020). PubMed: 31916488
- Guo D et al. Ontogenesis of the tear drainage system requires Prickle1-driven polarized basement membrane deposition. Development 147:N/A (2020). PubMed: 33144400
- Wang C et al. Endocytosis of flavivirus NS1 is required for NS1-mediated endothelial hyperpermeability and is abolished by a single N-glycosylation site mutation. PLoS Pathog 15:e1007938 (2019). PubMed: 31356638
- Hua Y et al. Oligomerization of Frizzled and LRP5/6 protein initiates intracellular signaling for the canonical WNT/ß-catenin pathway. J Biol Chem 293:19710-19724 (2018). PubMed: 30361437
- Bridgewater RE et al. Extracellular matrix promotes clathrin-dependent endocytosis of prolactin and STAT5 activation in differentiating mammary epithelial cells. Sci Rep 7:4572 (2017). WB . PubMed: 28676702