Recombinant Anti-CLCA1 antibody [EPR12254-88] (ab180851)
![Western blot - Anti-CLCA1 antibody [EPR12254-88] (ab180851)](https://www.abcam.com/ps/products/180/ab180851/Images/ab180851-296795-anti-clca1-antibody-epr12254-88-western-blot.jpg "Western blot - Anti-CLCA1 antibody [EPR12254-88] (ab180851)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12254-88] to CLCA1
- Suitable for: Flow Cyt (Intra), IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-CLCA1 antibody [EPR12254-88]
See all CLCA1 primary antibodies -
Description
Rabbit monoclonal [EPR12254-88] to CLCA1 -
Host species
Rabbit -
Specificity
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. -
Tested applications
Suitable for: Flow Cyt (Intra), IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Human CLCA1. The exact sequence is proprietary.
Database link: A8K7I4 -
Positive control
- Human colon cancer and human small intestine lysates; human colon tissue; A431 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.21% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12254-88 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab180851 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
Use at an assay dependent concentration.
|
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| IHC-P | (1) |
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. For unpurified use at 1/100 - 1/250. |
| WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 100 kDa.
|
| ICC/IF | (1) |
1/50 - 1/100.
|
| Notes |
|---|
|
Flow Cyt (Intra)
Use at an assay dependent concentration. |
|
IHC-P
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. For unpurified use at 1/100 - 1/250. |
|
WB
1/1000 - 1/10000. Predicted molecular weight: 100 kDa. |
|
ICC/IF
1/50 - 1/100. |
Target
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Function
May be involved in mediating calcium-activated chloride conductance. May play critical roles in goblet cell metaplasia, mucus hypersecretion, cystic fibrosis and AHR. May be involved in the regulation of mucus production and/or secretion by goblet cells. Involved in the regulation of tissue inflammation in the innate immune response. May play a role as a tumor suppressor. Induces MUC5AC. -
Tissue specificity
Highly expressed in small intestine and colon namely in intestinal basal crypt epithelia and goblet cells, and appendix. Weakly expressed in uterus, testis and kidney. Expressed in the airways epithelium of both asthmatic andhealthy patients. Expressed in the bronchial epithelium, especially in mucus-producing goblet cells. Expressed in normal turbinate mucosa and nasal polyp. Expressed in. -
Sequence similarities
Belongs to the CLCR family.
Contains 1 VWFA domain. -
Post-translational
modificationsGlycosylated.
The 125-kDa product is processed and yields to two cell-surface-associated subunits, a 90-kDa protein and a group of 37-to 41-kDa proteins. -
Cellular localization
Secreted > extracellular space. Cell membrane. Protein that remains attached to the plasma membrane appeared to be predominantly localized to microvilli. - Information by UniProt
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Database links
- Entrez Gene: 1179 Human
- Entrez Gene: 23844 Mouse
- Entrez Gene: 308015 Rat
- Omim: 603906 Human
- SwissProt: A8K7I4 Human
- SwissProt: Q9D7Z6 Mouse
- Unigene: 194659 Human
- Unigene: 33483 Mouse
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Alternative names
- CaCC antibody
- CaCC-1 antibody
- CACC1 antibody
see all
Images
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Western blot - Anti-CLCA1 antibody [EPR12254-88] (ab180851)Anti-CLCA1 antibody [EPR12254-88] (ab180851) at 1/10000 dilution (purified) + Human small intestine lysates at 15 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 100 kDaBlocking and diluting buffer: 5% NFDM/TBST
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![Flow Cytometry (Intracellular) - Anti-CLCA1 antibody [EPR12254-88] (ab180851)](https://www.abcam.com/ps/products/180/ab180851/Images/ab180851-297993-anti-clca1-antibody-epr12254-88-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-CLCA1 antibody [EPR12254-88] (ab180851)Intracellular Flow Cytometry analysis of A431 (Human epidermoid carcinoma epithelial cell) cells labeling CLCA1 with purified ab180851 at 1/20 dilution (10µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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![Immunocytochemistry/ Immunofluorescence - Anti-CLCA1 antibody [EPR12254-88] (ab180851)](https://www.abcam.com/ps/products/180/ab180851/Images/ab180851-297944-anti-clca1-antibody-epr12254-88-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CLCA1 antibody [EPR12254-88] (ab180851)Immunocytochemistry/ Immunofluorescence analysis of SW480 (Human colorectal adenocarcinoma epithelial cell) cells labeling CLCA1 with Purified ab180851 at 1:100 dilution. Cells were fixed in 100% Methanol. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLCA1 antibody [EPR12254-88] (ab180851)](https://www.abcam.com/ps/products/180/ab180851/Images/ab180851-297717-anti-clca1-antibody-epr12254-88-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLCA1 antibody [EPR12254-88] (ab180851)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human small intestine tissue sections labeling CLCA1 with Purified ab180851 at 1:4000 dilution (0.07 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
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![Western blot - Anti-CLCA1 antibody [EPR12254-88] (ab180851)](https://www.abcam.com/ps/products/180/ab180851/Images/ab180851-296796-anti-clca1-antibody-epr12254-88-western-blot.jpg)
Western blot - Anti-CLCA1 antibody [EPR12254-88] (ab180851)All lanes : Anti-CLCA1 antibody [EPR12254-88] (ab180851) at 1/50000 dilution (purified)
Lane 1 : Rat small intestine lysates
Lane 2 : Mouse small intestine lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 100 kDaBlocking and diluting buffer: 5% NFDM/TBST
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![Immunocytochemistry/ Immunofluorescence - Anti-CLCA1 antibody [EPR12254-88] (ab180851)](https://www.abcam.com/ps/products/180/ab180851/Images/ab180851-209680-anti-clca1-antibody-epr12254-88-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CLCA1 antibody [EPR12254-88] (ab180851)Immunofluorescence analysis of A431 cells labeling CLCA1 using unpurified ab180851 at 1/50 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLCA1 antibody [EPR12254-88] (ab180851)](https://www.abcam.com/ps/products/180/ab180851/Images/ab180851-209679-anti-clca1-antibody-epr12254-88-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLCA1 antibody [EPR12254-88] (ab180851)Immunohistochemical analysis of paraffin-embedded human colon tissue labeling CLCA1 using unpurified ab180851 at 1/100 dilution.
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![Western blot - Anti-CLCA1 antibody [EPR12254-88] (ab180851)](https://www.abcam.com/ps/products/180/ab180851/Images/ab180851-209678-anti-clca1-antibody-epr12254-88-western-blot.jpg)
Western blot - Anti-CLCA1 antibody [EPR12254-88] (ab180851)All lanes : Anti-CLCA1 antibody [EPR12254-88] (ab180851) at 1/1000 dilution (unpurified)
Lane 1 : Human colon cancer lysates
Lane 2 : Human small intestine lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 100 kDa -
![Anti-CLCA1 antibody [EPR12254-88] (ab180851)](https://www.abcam.com/ps/products/180/ab180851/Images/ab180851-9-benefits-of-recombinant-antibodies.png)
Anti-CLCA1 antibody [EPR12254-88] (ab180851)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (13)
ab180851 has been referenced in 13 publications.
- Mahood TH et al. Integrating Proteomes for Lung Tissues and Lavage Reveals Pathways That Link Responses in Allergen-Challenged Mice. ACS Omega 6:1171-1189 (2021). PubMed: 33490776
- Centeio R et al. CLCA1 Regulates Airway Mucus Production and Ion Secretion Through TMEM16A. Int J Mol Sci 22:N/A (2021). PubMed: 34066250
- Lee HJ et al. Chloride channel accessory 1 integrates chloride channel activity and mTORC1 in aging-related kidney injury. Aging Cell 20:e13407 (2021). PubMed: 34118180
- Nyström EEL et al. Calcium-activated chloride channel regulator 1 (CLCA1) forms non-covalent oligomers in colonic mucus and has mucin 2-processing properties. J Biol Chem 294:17075-17089 (2019). PubMed: 31570526
- Meldrum K et al. Cerium dioxide nanoparticles exacerbate house dust mite induced type II airway inflammation. Part Fibre Toxicol 15:24 (2018). PubMed: 29792201