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Anti-Collagen I + II + III + IV + V antibody (ab36064)

Reviews (1)Q&A (3)References (5)

Key features and details

  • Rabbit polyclonal to Collagen I + II + III + IV + V
  • Suitable for: ELISA, IHC-Fr, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-Collagen I antibody [EPR7785] (ab138492)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-Collagen I + II + III + IV + V antibody

  • Description

    Rabbit polyclonal to Collagen I + II + III + IV + V

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ELISA, IHC-Fr, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Full length native Collagen I + II + III + IV + V proteins (purified) from human placenta.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.50
    Constituents: 0.1% Dextran, 0.87% Sodium chloride, 0.164% Sodium phosphate, 0.1% Mannitol
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    This antibody is affinity isolated. It was purified by affinity chromatography on immobilized Collagen types I-V.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab36064 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
Notes
ELISA
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.

Target

  • Function

    Type I collagen is a member of group I collagen (fibrillar forming collagen).

  • Tissue specificity

    Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.

  • Involvement in disease

    Caffey disease
    Ehlers-Danlos syndrome, classic type
    Ehlers-Danlos syndrome 7A
    Osteogenesis imperfecta 1
    Osteogenesis imperfecta 2
    Osteogenesis imperfecta 3
    Osteogenesis imperfecta 4
    Osteoporosis
    A chromosomal aberration involving COL1A1 is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGF.

  • Sequence similarities

    Belongs to the fibrillar collagen family.
    Contains 1 fibrillar collagen NC1 domain.
    Contains 1 VWFC domain.

  • Domain

    The C-terminal propeptide, also known as COLFI domain, have crucial roles in tissue growth and repair by controlling both the intracellular assembly of procollagen molecules and the extracellular assembly of collagen fibrils. It binds a calcium ion which is essential for its function.

  • Post-translational
    modifications

    Proline residues at the third position of the tripeptide repeating unit (G-X-P) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-P-X) are hydroxylated in some of the chains.
    O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.

  • Cellular localization

    Secreted, extracellular space, extracellular matrix.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Alpha-1 type I collagen antibody
    • CO1A1_HUMAN antibody
    • COL1A1 antibody
    • COL2A1 antibody
    • COL3A1 antibody
    • COL4A1 antibody
    • COL5A1 antibody
    • Collagen alpha 1 chain antibody
    • Collagen alpha 1(I) chain antibody
    • Collagen alpha 1(II) chain antibody
    • Collagen alpha 1(III) chain antibody
    • Collagen alpha 1(IV) chain antibody
    • Collagen alpha 1(V) chain antibody
    • Collagen alpha-1(I) chain antibody
    • Collagen I alpha 1 polypeptide antibody
    • Collagen II alpha 1 polypeptide antibody
    • Collagen IV alpha 1 polypeptide antibody
    • Collagen type I alpha 1 antibody
    • Collagen type II alpha 1 antibody
    • Collagen type III alpha 1 antibody
    • Collagen type IV alpha 1 antibody
    • Collagen type V alpha 1 antibody
    • EDS4A antibody
    see all

References (5)

Publishing research using ab36064? Please let us know so that we can cite the reference in this datasheet.

ab36064 has been referenced in 5 publications.

  • Lau S  et al. Venous and Arterial Endothelial Cells from Human Umbilical Cords: Potential Cell Sources for Cardiovascular Research. Int J Mol Sci 22:N/A (2021). PubMed: 33478148
  • Luong B  et al. The vacuolar-type ATPase inhibitor archazolid increases tumor cell adhesion to endothelial cells by accumulating extracellular collagen. PLoS One 13:e0203053 (2018). PubMed: 30204757
  • Schwenk R  et al. The pretubulysin-induced exposure of collagen is caused by endothelial cell retraction that results in an increased adhesion and decreased transmigration of tumor cells. Oncotarget 8:77622-77633 (2017). PubMed: 29100413
  • Sur S  et al. Synergistic regulation of cerebellar Purkinje neuron development by laminin epitopes and collagen on an artificial hybrid matrix construct. Biomater Sci 2:903-914 (2014). PubMed: 25530849
  • Trescher K  et al. Test system for evaluating the influence of polymer properties on primary human keratinocytes and fibroblasts in mono- and coculture. J Biotechnol 166:58-64 (2013). PubMed: 23643480

Customer reviews and Q&As

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1-4 of 4 Abreviews or Q&A

Western blot abreview for Anti-Collagen I + II + III + IV + V antibody

 Below Average
Abreviews
abreview image
Application
Western blot
Sample
Human Tissue lysate - whole (Liver)
Loading amount
300 µg
Specification
Liver
Gel Running Conditions
Reduced Denaturing (4-12% precast)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 15 2012

Question

Our order was placed before you brought your testing program to my attention. Confirmation # ***********

Read More
Answer

Thank you for your reply. Below is the discount for testing ab36064.

Please let me know if there is anything else I can help you with.

DISCOUNT CODE: *********
Expiration date: ************

I am very pleased to hear you would like to accept our offer and test ab36064 in western blot. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview forwestern blot and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

Question

Thank you for your response a few weeks ago. We went ahead and purchased the antibody (ab36064) and are in the process of testing it for 2 new applications (immunoprecipitation of collagen molecules from human blood using Invitrogen’s Dynabead system and Western Blot from human serum). Per your testing policy, I would appreciate a personal discount code.



https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount

Read More
Answer

Thank you for contacting Abcam.

The testing discount codes are normally issued before you purchase the antibody, this is so we can track it in our system. However in this case I will make an exception and issue the code post purchase. To do this, could you please provide me with the Abcam order number for ab36064, so that I can generate a code.

I look forward to your reply and issuing the code.

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Question

I am interested in a non-specific antibody for immunoprecipitating collagen peptides from human serum. Can you give me your impressions on whether this antibody is suitable? Do you know what the epitope(s) are, and whether the antibody binding depends on the collagen having an intact triple helix structure? Along those lines, on a western blot, would reducing vs. non-reducing conditions affect the detection?

Read More
Answer

Thank you for contacting Abcam.
I have spoken to the lab about the epitopes that are recognized by ab36064 and the epitope has not been mapped for this antibody and because it is a polyclonal antibody, it will recognize multiple epitopes.
I believe it would be suitable for your needs since it does recognize multiply collagen family members. As to whether using this antibody under reducing conditions would affect it, I cannot say for certain, but I do not think it will. Although since we have not tested this antibody in western blot, we cannot guarantee that it will work, therefore you could take advantage of our testing discount program:
https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount

Please let me know if there is anything else I can help you with.

Read More

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