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    products/primary-antibodies/collagen-i-antibody-epr22209-75-ab255809.pdf

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Signal Transduction Cytoskeleton / ECM Extracellular Matrix ECM Proteins Collagen
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RecombinantRabMAb

Recombinant Anti-Collagen I antibody [EPR22209-75] (ab255809)

  • Datasheet
  • SDS
  • Certificate of Compliance
Submit a review Submit a question References (8)

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Western blot - Anti-Collagen I antibody [EPR22209-75] (ab255809)
  • Western blot - Anti-Collagen I antibody [EPR22209-75] (ab255809)
  • Western blot - Anti-Collagen I antibody [EPR22209-75] (ab255809)
  • Anti-Collagen I antibody [EPR22209-75] (ab255809)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22209-75] to Collagen I
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

You may also be interested in

Protein
Product image
Recombinant Human Collagen I protein (ab158152)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Knockout
Product image
Human COL1A1 (PICP) knockout U-2 OS cell line (ab273846)

View more associated products

Overview

  • Product name

    Anti-Collagen I antibody [EPR22209-75]
    See all Collagen I primary antibodies
  • Description

    Rabbit monoclonal [EPR22209-75] to Collagen I
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: NIH/3T3, rat lung, mouse kidney and mouse lung culture supernatants; HFF-1 and MRC-5 whole cell lysates and culture supernatants.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR22209-75
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Proteins
    • Collagen
    • Stem Cells
    • Mesenchymal Stem Cells
    • Osteogenesis

Associated products

  • Alternative Versions

    • Anti-Collagen I antibody [EPR22209-75] - BSA and Azide free (ab256541)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Recombinant Protein

    • Recombinant Human Collagen I protein (ab158152)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab255809 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Detects a band of approximately 35, 220 kDa (predicted molecular weight: 139 kDa).

Positive Control: Hu stomach, skin and adrenal gland tissue lysates.

Acid or enzyme treatment with pepsin is a better method to isolate collagen. Continuous refrigeration throughout collagen extraction is important to avoid degradation and denaturation. Take care with pH, temperature, and concentration to avoid collagen polymerization.

Notes
WB
1/1000. Detects a band of approximately 35, 220 kDa (predicted molecular weight: 139 kDa).

Positive Control: Hu stomach, skin and adrenal gland tissue lysates.

Acid or enzyme treatment with pepsin is a better method to isolate collagen. Continuous refrigeration throughout collagen extraction is important to avoid degradation and denaturation. Take care with pH, temperature, and concentration to avoid collagen polymerization.

Application notes
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.

Target

  • Function

    Type I collagen is a member of group I collagen (fibrillar forming collagen).
  • Tissue specificity

    Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
  • Involvement in disease

    Defects in COL1A1 are the cause of Caffey disease (CAFFD) [MIM:114000]; also known as infantile cortical hyperostosis. Caffey disease is characterized by an infantile episode of massive subperiosteal new bone formation that typically involves the diaphyses of the long bones, mandible, and clavicles. The involved bones may also appear inflamed, with painful swelling and systemic fever often accompanying the illness. The bone changes usually begin before 5 months of age and resolve before 2 years of age.
    Defects in COL1A1 are a cause of Ehlers-Danlos syndrome type 1 (EDS1) [MIM:130000]; also known as Ehlers-Danlos syndrome gravis. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS1 is the severe form of classic Ehlers-Danlos syndrome.
    Defects in COL1A1 are the cause of Ehlers-Danlos syndrome type 7A (EDS7A) [MIM:130060]; also known as autosomal dominant Ehlers-Danlos syndrome type VII. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS7A is marked by bilateral congenital hip dislocation, hyperlaxity of the joints, and recurrent partial dislocations.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 1 (OI1) [MIM:166200]. A dominantly inherited connective tissue disorder characterized by bone fragility and blue sclerae. Osteogenesis imperfecta type 1 is non-deforming with normal height or mild short stature, and no dentinogenesis imperfecta.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 2A (OI2A) [MIM:166210]; also known as osteogenesis imperfecta congenita. A connective tissue disorder characterized by bone fragility, with many perinatal fractures, severe bowing of long bones, undermineralization, and death in the perinatal period due to respiratory insufficiency.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 3 (OI3) [MIM:259420]. A connective tissue disorder characterized by progressively deforming bones, very short stature, a triangular face, severe scoliosis, grayish sclera, and dentinogenesis imperfecta.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 4 (OI4) [MIM:166220]; also known as osteogenesis imperfecta with normal sclerae. A connective tissue disorder characterized by moderately short stature, mild to moderate scoliosis, grayish or white sclera and dentinogenesis imperfecta.
    Genetic variations in COL1A1 are a cause of susceptibility to osteoporosis (OSTEOP) [MIM:166710]; also known as involutional or senile osteoporosis or postmenopausal osteoporosis. Osteoporosis is characterized by reduced bone mass, disruption of bone microarchitecture without alteration in the composition of bone. Osteoporotic bones are more at risk of fracture.
    Note=A chromosomal aberration involving COL1A1 is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGF.
  • Sequence similarities

    Belongs to the fibrillar collagen family.
    Contains 1 fibrillar collagen NC1 domain.
    Contains 1 VWFC domain.
  • Post-translational
    modifications

    Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some of the chains.
    O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Target information above from: UniProt accession P02452 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1277 Human
    • Entrez Gene: 1278 Human
    • Entrez Gene: 12842 Mouse
    • Entrez Gene: 12843 Mouse
    • Entrez Gene: 29393 Rat
    • Entrez Gene: 84352 Rat
    • Omim: 120150 Human
    • Omim: 120160 Human
    • SwissProt: P02452 Human
    • SwissProt: P08123 Human
    • SwissProt: P11087 Mouse
    • SwissProt: Q01149 Mouse
    • SwissProt: P02454 Rat
    • SwissProt: P02466 Rat
    • Unigene: 172928 Human
    • Unigene: 681002 Human
    • Unigene: 277735 Mouse
    • Unigene: 458212 Mouse
    • Unigene: 2953 Rat
    see all
  • Alternative names

    • Alpha 1 type I collagen antibody
    • Alpha 2 type I collagen antibody
    • alpha 2 type I procollagen antibody
    • alpha 2(I) procollagen antibody
    • alpha 2(I)-collagen antibody
    • Alpha-1 type I collagen antibody
    • alpha1(I) procollagen antibody
    • CO1A1_HUMAN antibody
    • COL1A1 antibody
    • COL1A2 antibody
    • collagen 1 antibody
    • collagen alpha 1 chain type I antibody
    • Collagen alpha-1(I) chain antibody
    • collagen alpha-1(I) chain preproprotein antibody
    • Collagen I alpha 1 polypeptide antibody
    • Collagen I alpha 2 polypeptide antibody
    • collagen of skin, tendon and bone, alpha-1 chain antibody
    • collagen of skin, tendon and bone, alpha-2 chain antibody
    • Collagen type I alpha 1 antibody
    • Collagen type I alpha 2 antibody
    • EDSC antibody
    • OI1 antibody
    • OI2 antibody
    • OI3 antibody
    • OI4 antibody
    • pro-alpha-1 collagen type 1 antibody
    • type I proalpha 1 antibody
    • type I procollagen alpha 1 chain antibody
    • Type I procollagen antibody
    see all

Images

  • Western blot - Anti-Collagen I antibody [EPR22209-75] (ab255809)
    Western blot - Anti-Collagen I antibody [EPR22209-75] (ab255809)
    All lanes : Anti-Collagen I antibody [EPR22209-75] (ab255809) at 1/1000 dilution

    Lane 1 : NIH/3T3 (mouse embryonic fibroblast) culture supernatant 10 ul
    Lane 2 : Mouse kidney organ culture supernatant 10 ul
    Lane 3 : Mouse lung organ culture supernatant 10 ul
    Lane 4 : Rat lung organ culture supernatant 10 ul

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 139 kDa
    Observed band size: 35 kDa why is the actual band size different from the predicted?



    Cleaved C-propeptide (35 kDa) are observed. The molecular weight observed is consistent with what has been described in the literature (PMID: 23940311).

    Exposure time: 26 seconds.

    Blocking and Diluting Buffer and concentration: 5% NFDM/TBST.

  • Western blot - Anti-Collagen I antibody [EPR22209-75] (ab255809)
    Western blot - Anti-Collagen I antibody [EPR22209-75] (ab255809)
    All lanes : Anti-Collagen I antibody [EPR22209-75] (ab255809) at 1/1000 dilution

    Lane 1 : A549 (Human lung carcinoma epithelial cell) whole cell lysate
    Lane 2 : MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysate
    Lane 3 : MDA-MB-231(Human breast adenocarcinoma epithelial cell) supernatant lysate
    Lane 4 : A431(Human epidermoid carcinoma epithelial cell) whole cell lysate
    Lane 5 : SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate
    Lane 6 : Human lung tissue lysate
    Lane 7 : Human liver tissue lysate
    Lane 8 : HFF-1(Human Skin fibroblast) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 139 kDa



    Blocking/Diluting buffer and concentration 5% NFDM/TBST.

    Exposure time:
    Lane1-7: 180 seconds
    Lane 8: 60 seconds

    We recommend loading higher amount of lysate or using lower antibody dilution.

  • Western blot - Anti-Collagen I antibody [EPR22209-75] (ab255809)
    Western blot - Anti-Collagen I antibody [EPR22209-75] (ab255809)
    All lanes : Anti-Collagen I antibody [EPR22209-75] (ab255809) at 1/1000 dilution

    Lane 1 : HFF-1 (human skin fibroblast) whole cell lysate at 20 µg
    Lane 2 : HFF-1 culture supernatant at 10 µl
    Lane 3 : MRC-5 (human lung fibroblast) whole cell lysate at 20 µg
    Lane 4 : MRC-5 culture supernatant at 10 µl

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 139 kDa
    Observed band size: 220,35 kDa why is the actual band size different from the predicted?



    Procollagen I (220 kDa) and cleaved C-propeptide (35 kDa) are observed. The molecular weight observed is consistent with what has been described in the literature (PMID: 23940311).

    Exposure time: 3 minutes.

    Blocking and Diluting Buffer and concentration: 5% NFDM/TBST.

  • Anti-Collagen I antibody [EPR22209-75] (ab255809)
    Anti-Collagen I antibody [EPR22209-75] (ab255809)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (8)

Publishing research using ab255809? Please let us know so that we can cite the reference in this datasheet.

ab255809 has been referenced in 8 publications.

  • Lin C  et al. Increased expression of osteopontin in subchondral bone promotes bone turnover and remodeling, and accelerates the progression of OA in a mouse model. Aging (Albany NY) 14:253-271 (2022). PubMed: 34982732
  • Liu Q  et al. Telmisartan inhibits bladder smooth muscle fibrosis in neurogenic bladder rats. Exp Ther Med 23:216 (2022). PubMed: 35126719
  • Luo J  et al. LOXL2 silencing suppresses angiotensin II-induced cardiac hypertrophy through the EMT process and TGF-β1/Smad3/NF-κB pathway. Iran J Basic Med Sci 25:964-969 (2022). PubMed: 36159334
  • Peng M  et al. Fufang Xueshuantong Improves Diabetic Cardiomyopathy by Regulating the Wnt/β-Catenin Pathway. Int J Endocrinol 2022:3919161 (2022). PubMed: 36237833
  • Lv S  et al. QiShenYiQi Pill Improves Myocardial Hypertrophy Caused by Pressure Overload in Rats. Evid Based Complement Alternat Med 2021:5536723 (2021). PubMed: 34221074
  • Sun C  et al. Adiponectin up-regulates the decrease of myocardial autophagic flux induced by ß1 -adrenergic receptor autoantibody partly dependent on AMPK. J Cell Mol Med 25:8464-8478 (2021). PubMed: 34322993
  • Qian W  et al. Identification and Validation of Potential Biomarkers and Pathways for Idiopathic Pulmonary Fibrosis by Comprehensive Bioinformatics Analysis. Biomed Res Int 2021:5545312 (2021). PubMed: 34285914
  • Gong W  et al. Network Pharmacology-Based Strategy for the Investigation of the Anti-Osteoporosis Effects and Underlying Mechanism of Zhuangguguanjie Formulation. Front Pharmacol 12:727808 (2021). PubMed: 34658868

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