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  1. Link

    products/primary-antibodies/collagen-i-antibody-epr22894-89-ab260043.pdf

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Signal Transduction Cytoskeleton / ECM Extracellular Matrix ECM Proteins Collagen
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RecombinantRabMAb

Recombinant Anti-Collagen I antibody [EPR22894-89] (ab260043)

  • Datasheet
  • SDS
  • Certificate of Compliance
Reviews (3) Submit a question References (17)

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Western blot - Anti-Collagen I antibody [EPR22894-89] (ab260043)
  • Western blot - Anti-Collagen I antibody [EPR22894-89] (ab260043)
  • Western blot - Anti-Collagen I antibody [EPR22894-89] (ab260043)
  • Immunoprecipitation - Anti-Collagen I antibody [EPR22894-89] (ab260043)
  • Flow Cytometry (Intracellular) - Anti-Collagen I antibody [EPR22894-89] (ab260043)
  • Flow Cytometry (Intracellular) - Anti-Collagen I antibody [EPR22894-89] (ab260043)
  • Anti-Collagen I antibody [EPR22894-89] (ab260043)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22894-89] to Collagen I
  • Suitable for: Flow Cyt (Intra), WB, IP
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Overview

  • Product name

    Anti-Collagen I antibody [EPR22894-89]
    See all Collagen I primary antibodies
  • Description

    Rabbit monoclonal [EPR22894-89] to Collagen I
  • Host species

    Rabbit
  • Specificity

    Compared with ab260043, ab255809 has lower background. We recommend ab255809 as an alternative for western blotting.
  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IPmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: NIH/3T3 culture supernatant, Rat lung organ culture supernatant. Human lung tissue lysate, HFF-1 whole cell lysate and culture supernatant, A549, MDA-MB-231, A431 and SW480 whole cell lysates and MDA-MB-231 supernatant lysate. IP: Mouse lung lysate. Flow Cyt (intra): HFF-1 and NIH/3T3 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR22894-89
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Proteins
    • Collagen
    • Stem Cells
    • Mesenchymal Stem Cells
    • Osteogenesis

Associated products

  • Alternative Versions

    • Anti-Collagen I antibody [EPR22894-89] - BSA and Azide free (ab264074)
  • Compatible Secondaries

    • VeriBlot for IP Detection Reagent (HRP) (ab131366)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human Collagen I protein (ab158152)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab260043 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/500.
WB (1)
1/1000. Predicted molecular weight: 139 kDa.

Positive Control: Hu stomach, skin and adrenal gland tissue lysates.

Acid or enzyme treatment with pepsin is a better method to isolate collagen. Continuous refrigeration throughout collagen extraction is important to avoid degradation and denaturation. Take care with pH, temperature, and concentration to avoid collagen polymerization.

IP
1/30.
Notes
Flow Cyt (Intra)
1/500.
WB
1/1000. Predicted molecular weight: 139 kDa.

Positive Control: Hu stomach, skin and adrenal gland tissue lysates.

Acid or enzyme treatment with pepsin is a better method to isolate collagen. Continuous refrigeration throughout collagen extraction is important to avoid degradation and denaturation. Take care with pH, temperature, and concentration to avoid collagen polymerization.

IP
1/30.
Application notes
Is unsuitable for ICC/IF or IHC-P.

Target

  • Function

    Type I collagen is a member of group I collagen (fibrillar forming collagen).
  • Tissue specificity

    Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
  • Involvement in disease

    Defects in COL1A1 are the cause of Caffey disease (CAFFD) [MIM:114000]; also known as infantile cortical hyperostosis. Caffey disease is characterized by an infantile episode of massive subperiosteal new bone formation that typically involves the diaphyses of the long bones, mandible, and clavicles. The involved bones may also appear inflamed, with painful swelling and systemic fever often accompanying the illness. The bone changes usually begin before 5 months of age and resolve before 2 years of age.
    Defects in COL1A1 are a cause of Ehlers-Danlos syndrome type 1 (EDS1) [MIM:130000]; also known as Ehlers-Danlos syndrome gravis. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS1 is the severe form of classic Ehlers-Danlos syndrome.
    Defects in COL1A1 are the cause of Ehlers-Danlos syndrome type 7A (EDS7A) [MIM:130060]; also known as autosomal dominant Ehlers-Danlos syndrome type VII. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS7A is marked by bilateral congenital hip dislocation, hyperlaxity of the joints, and recurrent partial dislocations.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 1 (OI1) [MIM:166200]. A dominantly inherited connective tissue disorder characterized by bone fragility and blue sclerae. Osteogenesis imperfecta type 1 is non-deforming with normal height or mild short stature, and no dentinogenesis imperfecta.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 2A (OI2A) [MIM:166210]; also known as osteogenesis imperfecta congenita. A connective tissue disorder characterized by bone fragility, with many perinatal fractures, severe bowing of long bones, undermineralization, and death in the perinatal period due to respiratory insufficiency.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 3 (OI3) [MIM:259420]. A connective tissue disorder characterized by progressively deforming bones, very short stature, a triangular face, severe scoliosis, grayish sclera, and dentinogenesis imperfecta.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 4 (OI4) [MIM:166220]; also known as osteogenesis imperfecta with normal sclerae. A connective tissue disorder characterized by moderately short stature, mild to moderate scoliosis, grayish or white sclera and dentinogenesis imperfecta.
    Genetic variations in COL1A1 are a cause of susceptibility to osteoporosis (OSTEOP) [MIM:166710]; also known as involutional or senile osteoporosis or postmenopausal osteoporosis. Osteoporosis is characterized by reduced bone mass, disruption of bone microarchitecture without alteration in the composition of bone. Osteoporotic bones are more at risk of fracture.
    Note=A chromosomal aberration involving COL1A1 is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGF.
  • Sequence similarities

    Belongs to the fibrillar collagen family.
    Contains 1 fibrillar collagen NC1 domain.
    Contains 1 VWFC domain.
  • Post-translational
    modifications

    Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some of the chains.
    O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Target information above from: UniProt accession P02452 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1277 Human
    • Entrez Gene: 1278 Human
    • Entrez Gene: 12842 Mouse
    • Entrez Gene: 12843 Mouse
    • Entrez Gene: 29393 Rat
    • Entrez Gene: 84352 Rat
    • Omim: 120150 Human
    • Omim: 120160 Human
    • SwissProt: P02452 Human
    • SwissProt: P08123 Human
    • SwissProt: P11087 Mouse
    • SwissProt: Q01149 Mouse
    • SwissProt: P02454 Rat
    • SwissProt: P02466 Rat
    • Unigene: 172928 Human
    • Unigene: 681002 Human
    • Unigene: 277735 Mouse
    • Unigene: 458212 Mouse
    • Unigene: 2953 Rat
    see all
  • Alternative names

    • Alpha 1 type I collagen antibody
    • Alpha 2 type I collagen antibody
    • alpha 2 type I procollagen antibody
    • alpha 2(I) procollagen antibody
    • alpha 2(I)-collagen antibody
    • Alpha-1 type I collagen antibody
    • alpha1(I) procollagen antibody
    • CO1A1_HUMAN antibody
    • COL1A1 antibody
    • COL1A2 antibody
    • collagen 1 antibody
    • collagen alpha 1 chain type I antibody
    • Collagen alpha-1(I) chain antibody
    • collagen alpha-1(I) chain preproprotein antibody
    • Collagen I alpha 1 polypeptide antibody
    • Collagen I alpha 2 polypeptide antibody
    • collagen of skin, tendon and bone, alpha-1 chain antibody
    • collagen of skin, tendon and bone, alpha-2 chain antibody
    • Collagen type I alpha 1 antibody
    • Collagen type I alpha 2 antibody
    • EDSC antibody
    • OI1 antibody
    • OI2 antibody
    • OI3 antibody
    • OI4 antibody
    • pro-alpha-1 collagen type 1 antibody
    • type I proalpha 1 antibody
    • type I procollagen alpha 1 chain antibody
    • Type I procollagen antibody
    see all

Images

  • Western blot - Anti-Collagen I antibody [EPR22894-89] (ab260043)
    Western blot - Anti-Collagen I antibody [EPR22894-89] (ab260043)
    All lanes : ab260043 and ab255809 at 1/1000 dilution

    Lane 1 : A549 (Human lung carcinoma epithelial cell) whole cell lysate
    Lane 2 : MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysate
    Lane 3 : MDA-MB-231(Human breast adenocarcinoma epithelial cell) supernatant lysate
    Lane 4 : A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate
    Lane 5 : SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 139 kDa
    Observed band size: 220,35 kDa why is the actual band size different from the predicted?



    Blocking buffer and concentration: 5% NFDM/TBST. 

    Diluting buffer and concentration: 5% NFDM/TBST.

    Exposure Time: ab260043: 20 seconds, ab255809: 180 seconds.

    ab181602 was used as loading control.

    Compared with ab260043, ab255809 has lower background. We recommend ab255809 as an alternative for western blotting.

    For better use ab255809, we recommend loading higher amount of lysate or using lower antibody dilution. 

  • Western blot - Anti-Collagen I antibody [EPR22894-89] (ab260043)
    Western blot - Anti-Collagen I antibody [EPR22894-89] (ab260043)
    All lanes : Anti-Collagen I antibody [EPR22894-89] (ab260043) at 1/5000 dilution

    Lane 1 : HFF-1 (human skin fibroblast) whole cell lysate at 20 µg
    Lane 2 : HFF-1 culture supernatant at 10 µl

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/25000 dilution

    Predicted band size: 139 kDa
    Observed band size: 220,35 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Procollagen I (220 kDa) and cleaved C-propeptide (35 kDa) are observed.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 23940311). 

    Exposure time: Lane 1: 1 second; Lane 2: 3 seconds.

  • Western blot - Anti-Collagen I antibody [EPR22894-89] (ab260043)
    Western blot - Anti-Collagen I antibody [EPR22894-89] (ab260043)
    All lanes : Anti-Collagen I antibody [EPR22894-89] (ab260043) at 1/1000 dilution

    Lane 1 : NIH/3T3 (mouse embryonic fibroblast) culture supernatant at 10 µl
    Lane 2 : Rat lung organ culture supernatant at 10 µl
    Lane 3 : Human lung tissue lysate at 10 µg

    Secondary
    Lanes 1-2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/25000 dilution
    Lane 3 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

    Predicted band size: 139 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Cleaved collagen I C-propeptide (35 kDa) is observed.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 23940311). 

    Exposure time: Lanes 1-2: 3 seconds; Lane 3: 136 seconds.

  • Immunoprecipitation - Anti-Collagen I antibody [EPR22894-89] (ab260043)
    Immunoprecipitation - Anti-Collagen I antibody [EPR22894-89] (ab260043)

    Collagen I was immunoprecipitated from 0.35 mg mouse lung lysate 10µg with ab260043 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab260043 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.

    Lane 1: Mouse lung lysate 10µg

    Lane 2: ab260043 IP in mouse lung lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab260043 in mouse lung lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

     

  • Flow Cytometry (Intracellular) - Anti-Collagen I antibody [EPR22894-89] (ab260043)
    Flow Cytometry (Intracellular) - Anti-Collagen I antibody [EPR22894-89] (ab260043)

    Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) cells labeling Collagen I with ab260043 at 1/500 (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. 

     

     

  • Flow Cytometry (Intracellular) - Anti-Collagen I antibody [EPR22894-89] (ab260043)
    Flow Cytometry (Intracellular) - Anti-Collagen I antibody [EPR22894-89] (ab260043)

    Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HFF-1 (Human skin fibroblast) cells labeling Collagen I with ab260043 at 1/500 (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. 

     

     

  • Anti-Collagen I antibody [EPR22894-89] (ab260043)
    Anti-Collagen I antibody [EPR22894-89] (ab260043)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (17)

Publishing research using ab260043? Please let us know so that we can cite the reference in this datasheet.

ab260043 has been referenced in 17 publications.

  • Tang W  et al. METTL3 enhances NSD2 mRNA stability to reduce renal impairment and interstitial fibrosis in mice with diabetic nephropathy. BMC Nephrol 23:124 (2022). WB ; Mouse . PubMed: 35354439
  • Chen H  et al. MicroRNA-124-3p affects myogenic differentiation of adipose-derived stem cells by targeting Caveolin-1 during pelvic floor dysfunction in Sprague Dawley rats. Ann Transl Med 9:161 (2021). PubMed: 33569463
  • Tang CJ  et al. Metformin prevents PFKFB3-related aerobic glycolysis from enhancing collagen synthesis in lung fibroblasts by regulating AMPK/mTOR pathway. Exp Ther Med 21:581 (2021). PubMed: 33850553
  • Dong L  et al. Hypoxic hUCMSC-derived extracellular vesicles attenuate allergic airway inflammation and airway remodeling in chronic asthma mice. Stem Cell Res Ther 12:4 (2021). PubMed: 33407872
  • Li J  et al. The MIR155 host gene/microRNA-627/HMGB1/NF-?B loop modulates fibroblast proliferation and extracellular matrix deposition. Life Sci 269:119085 (2021). PubMed: 33482190
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-3 of 3 Abreviews or Q&A

Western blot abreview for Anti-Collagen I antibody [EPR22894-89]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (Retina)
Gel Running Conditions
Reduced Denaturing (10% SDS-Gel)
Loading amount
10 µg
Specification
Retina
Blocking step
Protein-Free T20 blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 02 2022

Immunocytochemistry/ Immunofluorescence abreview for Anti-Collagen I antibody [EPR22894-89]

Excellent
Abreviews
Abreviews
This product is known to not work in this application or species.
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human stromal cells)
Permeabilization
No
Specification
Human stromal cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Apr 05 2021

IHC - Wholemount abreview for Anti-Collagen I antibody [EPR22894-89]

Average
Abreviews
Abreviews
abreview image
Application
IHC - Wholemount
Sample
Mouse Tissue (Ear skin)
Specification
Ear skin
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 27 2021

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