Anti-Collagen IV antibody (ab6586)
Key features and details
- Rabbit polyclonal to Collagen IV
- Suitable for: ELISA, IHC-Fr, WB, IHC-P, IP, ICC/IF, IHC-FrFl, IHC-FoFr
- Reacts with: Mouse, Rat, Hamster, Cow, Dog, Human, Pig, Zebrafish, African green monkey, Chinese hamster, Syrian hamster
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
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Overview
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Product name
Anti-Collagen IV antibody
See all Collagen IV primary antibodies -
Description
Rabbit polyclonal to Collagen IV -
Host species
Rabbit -
Specificity
ab6586 is designed to bind specifically to NATIVE collagen epitopes composed of multiple subunit strands. Negligible cross-reactivity with Type I, II, III, V or VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible. -
Tested applications
Suitable for: ELISA, IHC-Fr, WB, IHC-P, IP, ICC/IF, IHC-FrFl, IHC-FoFrmore details -
Species reactivity
Reacts with: Mouse, Rat, Hamster, Cow, Dog, Human, Pig, Zebrafish, African green monkey, Chinese hamster, Syrian hamster
Predicted to work with: Mammals
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Immunogen
Full length native protein (purified) corresponding to Collagen IV. Collagen Type IV from human and bovine placenta. The immunogen maintains the native conformation of the protein.
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Positive control
- IHC-P: Human kidney, liver, lung, renal oncocytoma and skeletal muscle tissues.
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General notes
There are other recombinant monoclonal options, such as Recombinant Anti-Collagen IV antibody.
Abcam recommended secondaries - Goat Anti-Rabbit HRP (ab205718) and Goat Anti-Rabbit Alexa Fluor® 488 (ab150077).
See other anti-rabbit secondary antibodies that can be used with this antibody.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.8766% Sodium chloride, 0.424% Potassium phosphate -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. -
Primary antibody notes
This antibody is well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6586 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
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| IHC-Fr | (21) |
Use at an assay dependent concentration.
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| WB | (26) |
Use at an assay dependent concentration. Predicted molecular weight: 161 kDa.
This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions. |
| IHC-P | (36) |
1/15 - 1/400. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| IP | (3) |
Use at an assay dependent concentration.
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| ICC/IF | (23) |
Use at an assay dependent concentration. PubMed: 19933193
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| IF |
Use at an assay dependent concentration.
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| IHC-FrFl | (1) |
Use at an assay dependent concentration.
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| IHC-FoFr | (8) |
Use at an assay dependent concentration.
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| Notes |
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ELISA
Use at an assay dependent concentration. |
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IHC-Fr
Use at an assay dependent concentration. |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 161 kDa. This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions. |
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IHC-P
1/15 - 1/400. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
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IP
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. PubMed: 19933193 |
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IF
Use at an assay dependent concentration. |
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IHC-FrFl
Use at an assay dependent concentration. |
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IHC-FoFr
Use at an assay dependent concentration. |
Target
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Function
Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.
Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation. Inhibits expression of hypoxia-inducible factor 1alpha and ERK1/2 and p38 MAPK activation. Ligand for alpha1/beta1 integrin. -
Tissue specificity
Highly expressed in placenta. -
Involvement in disease
Defects in COL4A1 are a cause of brain small vessel disease with hemorrhage (BSVDH) [MIM:607595]. Brain small vessel diseases underlie 20 to 30 percent of ischemic strokes and a larger proportion of intracerebral hemorrhages. Inheritance is autosomal dominant.
Defects in COL4A1 are the cause of hereditary angiopathy with nephropathy aneurysms and muscle cramps (HANAC) [MIM:611773]. The clinical renal manifestations include hematuria and bilateral large cysts. Histologic analysis revealed complex basement membrane defects in kidney and skin. The systemic angiopathy appears to affect both small vessels and large arteries.
Defects in COL4A1 are a cause of porencephaly familial (PCEPH) [MIM:175780]. Porencephaly is a term used for any cavitation or cerebrospinal fluid-filled cyst in the brain. Porencephaly type 1 is usually unilateral and results from focal destructive lesions such as fetal vascular occlusion or birth trauma. Type 2, or schizencephalic porencephaly, is usually symmetric and represents a primary defect or arrest in the development of the cerebral ventricles. -
Sequence similarities
Belongs to the type IV collagen family.
Contains 1 collagen IV NC1 (C-terminal non-collagenous) domain. -
Domain
Alpha chains of type IV collagen have a non-collagenous domain (NC1) at their C-terminus, frequent interruptions of the G-X-Y repeats in the long central triple-helical domain (which may cause flexibility in the triple helix), and a short N-terminal triple-helical 7S domain. -
Post-translational
modificationsLysines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in all cases and bind carbohydrates.
Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Type IV collagens contain numerous cysteine residues which are involved in inter- and intramolecular disulfide bonding. 12 of these, located in the NC1 domain, are conserved in all known type IV collagens.
The trimeric structure of the NC1 domains is stabilized by covalent bonds between Lys and Met residues.
Proteolytic processing produces the C-terminal NC1 peptide, arresten. -
Cellular localization
Secreted > extracellular space > extracellular matrix > basement membrane. - Information by UniProt
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Database links
- Entrez Gene: 282191 Cow
- Entrez Gene: 317711 Cow
- Entrez Gene: 407107 Cow
- Entrez Gene: 508632 Cow
- Entrez Gene: 511602 Cow
- Entrez Gene: 1282 Human
- Entrez Gene: 1284 Human
- Entrez Gene: 1285 Human
see all -
Alternative names
- Arresten antibody
- BSVD antibody
- CO4A1_HUMAN antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody (ab6586)
Immunohistochemical analysis formalin-fixed paraffin-embedded human lung tissue labelling Collagen IV with ab6586 at 1/15 dilution for 1 hour at 37 °C followed by a ready to use Polymer-HRP, Rabbit/Mouse Detection Kit. Blocking: Peroxidase-Blocking Solution for 10 minutes. Substrate: DAB-Chromogen, Rabbit/Mouse. Staining/Results: basement membranes and vessels. Counterstained with hematoxylin for 15 seconds.
Heat induced epitope retrieval (HIER) using Tris-EDTA-citrate buffer pH 7.8 for 5 minutes.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody (ab6586)Immunohistochemical analysis of formalin-fixed paraffin-embedded human skeletal muscle cells labelling Collagen IV with ab6586 at 1/15 dilution for 1 hour at 37 °C followed by a ready to use Polymer-HRP, Rabbit/Mouse Detection Kit. Blocking: Peroxidase-Blocking Solution for 10 minutes. Substrate: DAB-Chromogen, Rabbit/Mouse. Staining/Results: cells surrounded by collagen IV fibers. Counterstained with hematoxylin for 15 seconds.
Heat induced epitope retrieval (HIER) using Tris-EDTA-citrate buffer pH 7.8 for 5 minutes.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody (ab6586)Immunohistochemical analysis of formalin-fixed paraffin-embedded human renal oncocytoma tissue labelling Collagen IV with ab6586 at 1/15 dilution for 1 hour at 37 °C followed by a ready to use Polymer-HRP, Rabbit/Mouse Detection Kit. Blocking: Peroxidase-Blocking Solution for 10 minutes. Substrate: DAB-Chromogen, Rabbit/Mouse. Staining/Results: dense collagen IV positive membranes surrounding tumor cell nests. Counterstained with hematoxylin for 15 seconds
Heat induced epitope retrieval (HIER) using Tris-EDTA-citrate buffer pH 7.8 for 5 minutes.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody (ab6586)Paraffin-embedded human kidney tissue stained for Collagen IV using ab6586 at 1/400 dilution in immunohistochemical analysis with strong staining observed in glomeruli.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody (ab6586)Paraffin-embedded human liver tissue stained for Collagen IV using ab6586 at 1/400 dilution in immunohistochemical analysis, strong staining was observed in the sinusoids.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (820)
ab6586 has been referenced in 820 publications.
- Liu K et al. Relaxin-2 Prevents Erectile Dysfunction by Cavernous Nerve, Endothelial and Histopathological Protection Effects in Rats with Bilateral Cavernous Nerve Injury. World J Mens Health 41:434-445 (2023). PubMed: 36047071
- Díaz-Flores L et al. Intussusceptive angiogenesis facilitated by microthrombosis has an important example in angiolipoma. An ultrastructural and immunohistochemical study. Histol Histopathol 38:29-46 (2023). PubMed: 35775452
- Wang Y et al. Resveratrol's neural protective effects for the injured embryoid body and cerebral organoid. BMC Pharmacol Toxicol 23:47 (2022). PubMed: 35820950
- Takiguchi M et al. Chondroitinase ABC Administration Facilitates Serotonergic Innervation of Motoneurons in Rats With Complete Spinal Cord Transection. Front Integr Neurosci 16:881632 (2022). PubMed: 35845919
- Gao M et al. Kidney ECM Pregel Nanoarchitectonics for Microarrays to Accelerate Harvesting Gene-Edited Porcine Primary Monoclonal Spheres. ACS Omega 7:23156-23169 (2022). PubMed: 35847249