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    products/primary-antibodies/collagen-vi-antibody-epr17072-ab182744.pdf

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Signal Transduction Cytoskeleton / ECM Extracellular Matrix ECM Proteins Collagen
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Collagen VI antibody [EPR17072] (ab182744)

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  • SDS
  • Certificate of Compliance
Reviews (2) Submit a question References (15)

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Multiplex immunohistochemistry - Anti-Collagen VI antibody [EPR17072] (ab182744)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen VI antibody [EPR17072] (ab182744)
  • Western blot - Anti-Collagen VI antibody [EPR17072] (ab182744)
  • Western blot - Anti-Collagen VI antibody [EPR17072] (ab182744)
  • Multiplex immunohistochemistry - Anti-Collagen VI antibody [EPR17072] (ab182744)
  • Immunocytochemistry/ Immunofluorescence - Anti-Collagen VI antibody [EPR17072] (ab182744)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen VI antibody [EPR17072] (ab182744)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen VI antibody [EPR17072] (ab182744)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen VI antibody [EPR17072] (ab182744)
  • Western blot - Anti-Collagen VI antibody [EPR17072] (ab182744)
  • Western blot - Anti-Collagen VI antibody [EPR17072] (ab182744)
  • Western blot - Anti-Collagen VI antibody [EPR17072] (ab182744)
  • Anti-Collagen VI antibody [EPR17072] (ab182744)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17072] to Collagen VI
  • Suitable for: WB, ICC/IF, IHC-P, mIHC
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 594 Alexa Fluor® 647 Carrier Free HRP

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Alexa Fluor® 488 Anti-CD8 alpha antibody [EP1150Y] (ab196462)
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Alexa Fluor® 488 Anti-Collagen VI antibody [EPR17072] (ab200429)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-Collagen VI antibody [EPR17072]
    See all Collagen VI primary antibodies
  • Description

    Rabbit monoclonal [EPR17072] to Collagen VI
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-P, mIHCmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human skeletal muscle, Human placenta, Human fetal brain, Human fetal heart, Human fetal kidney, Human fetal spleen, Mouse heart, Mouse kidney, Mouse spleen, Rat kidney and Rat spleen lysates; HEK293T, WI-38 and NIH/3T3 whole cell lysates. IHC-P: Human liver, Human cardiac muscle, Mouse kidney and Rat stomach tissues. mIHC: Human liver and Human prostate gland tissues. ICC/IF: HeLa cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR17072
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Proteins
    • Collagen
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Extracellular matrix
    • Other

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-Collagen VI antibody [EPR17072] (ab200429)
    • Alexa Fluor® 647 Anti-Collagen VI antibody [EPR17072] (ab200430)
    • HRP Anti-Collagen VI antibody [EPR17072] (ab200596)
    • Alexa Fluor® 594 Anti-Collagen VI antibody [EPR17072] (ab207292)
    • Anti-Collagen VI antibody [EPR17072] - Low endotoxin, Azide free (ab229450)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human COL6A1 knockout HEK-293T cell line (ab265060)
  • KO cell lysates

    • Human COL6A1 knockout HEK-293T cell lysate (ab256879)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab182744 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/2000. Detects a band of approximately 147 kDa (predicted molecular weight: 109 kDa).
ICC/IF
1/200.
IHC-P (1)
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
mIHC
1/500 - 1/1000.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Notes
WB
1/2000. Detects a band of approximately 147 kDa (predicted molecular weight: 109 kDa).
ICC/IF
1/200.
IHC-P
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
mIHC
1/500 - 1/1000.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Collagen VI acts as a cell-binding protein.
  • Involvement in disease

    Defects in COL6A1 are a cause of Bethlem myopathy (BM) [MIM:158810]. BM is a rare autosomal dominant proximal myopathy characterized by early childhood onset (complete penetrance by the age of 5) and joint contractures most frequently affecting the elbows and ankles.
    Defects in COL6A1 are a cause of Ullrich congenital muscular dystrophy (UCMD) [MIM:254090]; also known as Ullrich scleroatonic muscular dystrophy. UCMD is an autosomal recessive congenital myopathy characterized by muscle weakness and multiple joint contractures, generally noted at birth or early infancy. The clinical course is more severe than in Bethlem myopathy.
  • Sequence similarities

    Belongs to the type VI collagen family.
    Contains 3 VWFA domains.
  • Post-translational
    modifications

    Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Target information above from: UniProt accession P12109 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1291 Human
    • Entrez Gene: 1292 Human
    • Entrez Gene: 1293 Human
    • Entrez Gene: 12833 Mouse
    • Entrez Gene: 12834 Mouse
    • Entrez Gene: 12835 Mouse
    • Entrez Gene: 294337 Rat
    • Entrez Gene: 361821 Rat
    • Omim: 120220 Human
    • Omim: 120240 Human
    • Omim: 120250 Human
    • SwissProt: P12109 Human
    • SwissProt: P12110 Human
    • SwissProt: P12111 Human
    • SwissProt: Q02788 Mouse
    • SwissProt: Q04857 Mouse
    • Unigene: 474053 Human
    • Unigene: 2509 Mouse
    see all
  • Alternative names

    • Alpha 1 (VI) chain (61 AA) antibody
    • CO6A1_HUMAN antibody
    • COL6A1 antibody
    • COL6A2 antibody
    • COL6A3 antibody
    • collagen 6 antibody
    • Collagen alpha 2(VI) chain antibody
    • Collagen alpha 3(VI) chain antibody
    • Collagen alpha-1(VI) chain antibody
    • collagen six antibody
    • Collagen type VI alpha 1 antibody
    • Collagen type VI alpha 2 antibody
    • Collagen type VI alpha 3 antibody
    • Collagen VI alpha 1 polypeptide antibody
    • Collagen VI alpha 2 polypeptide antibody
    • Collagen VI alpha 3 polypeptide antibody
    • CollagenVI antibody
    • Human mRNA for collagen VI alpha 2 C terminal globular domain antibody
    • OPLL antibody
    • PP3610 antibody
    see all

Images

  • Multiplex immunohistochemistry - Anti-Collagen VI antibody [EPR17072] (ab182744)
    Multiplex immunohistochemistry - Anti-Collagen VI antibody [EPR17072] (ab182744)

    Fluorescence multiplex immunohistochemical analysis of human prostate gland tissue (formalin/PFA-fixed paraffin-embedded section). Panel A: merged staining of anti-Collagen VI (ab271938, magenta; Opal™690), anti-Cytokeratin 5 (ab236216, green; Opal™520) and anti-Prostate Specific Antigen (ab76113, red; Opal™570) on human prostate gland tissue. Panel B: anti-Prostate Specific Antigen stained on luminal cells. Panel C: anti-Cytokeratin 5 stained on basal cells. Panel D: anti-Collagen VI stained on stroma. Opal Polymer HRP Ms + Rb was used as a secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. The section was incubated in three rounds of staining: in the order of ab271938 (1/500), ab236216 (1/400), and ab76113 (1/2000) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) was used for 20 mins. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Leica SP8 confocal microscope.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen VI antibody [EPR17072] (ab182744)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen VI antibody [EPR17072] (ab182744)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Collagen VI with ab182744 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Positive staining around sinusoidal endothelial basement membranes is observed. Counter stained with Hematoxylin.

    Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-Collagen VI antibody [EPR17072] (ab182744)
    Western blot - Anti-Collagen VI antibody [EPR17072] (ab182744)
    All lanes : Anti-Collagen VI antibody [EPR17072] (ab182744) at 1/1000 dilution

    Lane 1 : Wild-type HEK293T cell lysate
    Lane 2 : COL6A1 knockout HEK293T cell lysate
    Lane 3 : Human skeletal muscle tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 109 kDa
    Observed band size: 136 kDa why is the actual band size different from the predicted?



    Lanes 1-3: Merged signal (red and green). Green - ab182744 observed at 136 kDa. Red - loading control ab8245 observed at 36 kDa. 

     ab182744 Anti-Collagen VI antibody [EPR17072]  was shown to specifically react with Collagen VI antibody in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab265060 (knockout cell lysate ab256879) was used.  Wild-type and Collagen VI antibody knockout samples were subjected to SDS-PAGE.  ab182744 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Collagen VI antibody [EPR17072] (ab182744)
    Western blot - Anti-Collagen VI antibody [EPR17072] (ab182744)
    All lanes : Anti-Collagen VI antibody [EPR17072] (ab182744)

    Lane 1 : Wild-type HEK293 whole cell lysate
    Lane 2 : COL6A1 knockout HEK293 whole cell lysate
    Lane 3 : Human Skeletal Muscle whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 109 kDa



    Lanes 1 - 3: Merged signal (red and green). Green - ab182744 observed at 109 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab182744 was shown to recognize Collagen VI in wild-type HEK293 cells as signal was lost at the expected MW in COL6A1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and COL6A1 knockout samples were subjected to SDS-PAGE. Ab182744 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/2000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Multiplex immunohistochemistry - Anti-Collagen VI antibody [EPR17072] (ab182744)
    Multiplex immunohistochemistry - Anti-Collagen VI antibody [EPR17072] (ab182744)

    Multiplex immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue.

    Panel A:  Merged staining of Collagen VI (ab182744; green), anti-CD68 (ab213363; red) and anti-Lamin B1 (ab229025; magenta).

    Panel B: Anti-Collagen VI (green) stained on extracellular matrix.

    Panel C: Anti-CD68 (red) stained on Kupffer cells.

    Panel D: Anti-Lamin B1 (magenta) stained on nuclear envelope.

    Key protocol steps:  The section was incubated in three rounds of staining with ab182744 (1/1000 dilution), ab213363 (1/1000 dilution) and ab229025 (1/4000 dilution) for 30 mins at room temperature. Each round was followed by tyramide signal amplification with the appropriate fluorophore.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    DAPI was used as a nuclear counter stain. A ready-to-use anti-Rabbit and Mouse Polymer HRP was used as a secondary.

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Collagen VI antibody [EPR17072] (ab182744)
    Immunocytochemistry/ Immunofluorescence - Anti-Collagen VI antibody [EPR17072] (ab182744)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Collagen VI with ab182744 at 1/200 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution (green). Cytoplasm staining on HeLa cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

    The negative controls are as follows:-

    -ve control1 - ab182744 at 1/200 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2 - ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen VI antibody [EPR17072] (ab182744)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen VI antibody [EPR17072] (ab182744)

    Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling Collagen VI with ab182744 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Positive staining on Human cardiac sarcolemma and interstitium is observed. Counter stained with Hematoxylin.

    Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen VI antibody [EPR17072] (ab182744)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen VI antibody [EPR17072] (ab182744)

    Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Collagen VI with ab182744 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Positive staining around basement membranes of Mouse renal tubules is observed. Counter stained with Hematoxylin.

    Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen VI antibody [EPR17072] (ab182744)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen VI antibody [EPR17072] (ab182744)

    Immunohistochemical analysis of paraffin-embedded Rat stomach tissue labeling Collagen VI with ab182744 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Positive staining around Rat gastric epithelial basement membranes is observed. Counter stained with Hematoxylin.

    Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-Collagen VI antibody [EPR17072] (ab182744)
    Western blot - Anti-Collagen VI antibody [EPR17072] (ab182744)
    All lanes : Anti-Collagen VI antibody [EPR17072] (ab182744) at 1/20000 dilution

    Lane 1 : Human skeletal muscle lysate
    Lane 2 : WI-38 (Human fetal lung fibroblast cells) whole cell lysate
    Lane 3 : Human placenta lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 109 kDa
    Observed band size: 147 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Due to posttranslational modifications, observed MW is greater than the predicted MW.

  • Western blot - Anti-Collagen VI antibody [EPR17072] (ab182744)
    Western blot - Anti-Collagen VI antibody [EPR17072] (ab182744)
    All lanes : Anti-Collagen VI antibody [EPR17072] (ab182744) at 1/2000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human fetal heart lysate
    Lane 3 : Human fetal kidney lysate
    Lane 4 : Human fetal spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 109 kDa
    Observed band size: 147 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Due to posttranslational modifications, observed MW is greater than the predicted MW.

  • Western blot - Anti-Collagen VI antibody [EPR17072] (ab182744)
    Western blot - Anti-Collagen VI antibody [EPR17072] (ab182744)
    All lanes : Anti-Collagen VI antibody [EPR17072] (ab182744) at 1/2000 dilution

    Lane 1 : Mouse heart lysate
    Lane 2 : Mouse kidney lysate
    Lane 3 : Mouse spleen lysate
    Lane 4 : Rat kidney lysate
    Lane 5 : Rat spleen lysate
    Lane 6 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 109 kDa
    Observed band size: 147 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Due to posttranslational modifications, observed MW is greater than the predicted MW.

  • Anti-Collagen VI antibody [EPR17072] (ab182744)
    Anti-Collagen VI antibody [EPR17072] (ab182744)

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (15)

Publishing research using ab182744? Please let us know so that we can cite the reference in this datasheet.

ab182744 has been referenced in 15 publications.

  • Liu S  et al. miR-106b-5p targeting SIX1 inhibits TGF-ß1-induced pulmonary fibrosis and epithelial-mesenchymal transition in asthma through regulation of E2F1. Int J Mol Med 47:N/A (2021). PubMed: 33495833
  • Zhong J  et al. Use of RNA-sequencing to detect abnormal transcription of the collagen a-2 (VI) chain gene that can lead to Bethlem myopathy. Int J Mol Med 47:N/A (2021). PubMed: 33537799
  • Caetano AJ  et al. Defining human mesenchymal and epithelial heterogeneity in response to oral inflammatory disease. Elife 10:N/A (2021). PubMed: 33393902
  • Daquinag AC  et al. Glycosaminoglycan Modification of Decorin Depends on MMP14 Activity and Regulates Collagen Assembly. Cells 9:N/A (2020). PubMed: 33317052
  • Danalache M  et al. Biochemical changes of the pericellular matrix and spatial chondrocyte organization-Two highly interconnected hallmarks of osteoarthritis. J Orthop Res N/A:N/A (2020). PubMed: 32301522
View all Publications for this product

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Western blot abreview for Anti-Collagen VI antibody [EPR17072]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Cell lysate - whole cell (Mammary tumor)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
Mammary tumor
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 14 2017

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Collagen VI antibody [EPR17072]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM sodium citrate
Permeabilization
No
Specification
lung
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative
Formalin
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 10 2016

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