Recombinant Anti-Collagen X antibody [EPR13044] (ab182563)
![Western blot - Anti-Collagen X antibody [EPR13044] (ab182563)](https://www.abcam.com/ps/products/182/ab182563/Images/ab182563-324118-anti-collagen-x-antibody-epr13044-western-blot.jpg "Western blot - Anti-Collagen X antibody [EPR13044] (ab182563)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13044] to Collagen X
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Collagen X antibody [EPR13044]
See all Collagen X primary antibodies -
Description
Rabbit monoclonal [EPR13044] to Collagen X -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human fetal skin and placenta tissue lysates; Rat skin tissue lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13044 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab182563 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/2000. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa).
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| Notes |
|---|
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WB
1/1000 - 1/2000. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa). |
Target
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Function
Type X collagen is a product of hyperthrophic chondrotocytes and has been localized to presumptive mineralization zones of hyaline cartilage. -
Involvement in disease
Defects in COL10A1 are the cause of Schmid type metaphyseal chondrodysplasia (SMCD) [MIM:156500]. SMCD is a dominantly inherited disorder of the osseous skeleton. The cardinal features of the phenotype are mild short stature, coxa vara and a waddling gait. Radiography usually shows sclerosis of the ribs, flaring of the metaphyses, and a wide irregular growth plate, especially of the knees. A variant form of SMCD is spondylometaphyseal dysplasia Japanese type. It is characterized by spinal involvement comprising mild platyspondyly, vertebral body abnormalities, and end-plate irregularity. -
Sequence similarities
Contains 1 C1q domain. -
Post-translational
modificationsProlines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 1300 Human
- Entrez Gene: 12813 Mouse
- Entrez Gene: 25681 Rat
- Omim: 120110 Human
- SwissProt: Q03692 Human
- SwissProt: Q05306 Mouse
- Unigene: 520339 Human
- Unigene: 443177 Mouse
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Alternative names
- COAA1_HUMAN antibody
- Col10a 1 antibody
- COL10A1 antibody
see all
Images
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Western blot - Anti-Collagen X antibody [EPR13044] (ab182563)All lanes : Anti-Collagen X antibody [EPR13044] (ab182563) at 1/1000 dilution
Lane 1 : Human fetal skin tissue lysates
Lane 2 : Rat skin tissue lysates
Lane 3 : Mouse cartilage tissue lysates
Lane 4 : Human cartilage tissue lysates
Lane 5 : Rat cartilage tissue lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 66 kDa
Observed band size: 66 kDaExposure time
Lane 1: 6 seconds
Lane 2: 70 seconds
Lane 3 to 5: 10 secondsBlocking/Diluting buffer and concentration 5% NFDM/TBST
Collagen X is normally not expressed in human healthy cartilage but its expression is detected at protein and mRNA level in human osteoarthritic cartilage according to PMID 22178514 and 26861791.
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![Western blot - Anti-Collagen X antibody [EPR13044] (ab182563)](https://www.abcam.com/ps/products/182/ab182563/Images/ab182563-213941-anti-collagen-x-antibody-epr13044-western-blot.jpg)
Western blot - Anti-Collagen X antibody [EPR13044] (ab182563)All lanes : Anti-Collagen X antibody [EPR13044] (ab182563) at 1/1000 dilution
Lane 1 : Human fetal skin lysate
Lane 2 : Human placenta lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 66 kDa
Additional bands at: 66 kDa. We are unsure as to the identity of these extra bands. -
![Anti-Collagen X antibody [EPR13044] (ab182563)](https://www.abcam.com/ps/products/182/ab182563/Images/ab182563-3-benefits-of-recombinant-antibodies.png)
Anti-Collagen X antibody [EPR13044] (ab182563)
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (22)
ab182563 has been referenced in 22 publications.
- Kamakura T et al. Collagen X Is Dispensable for Hypertrophic Differentiation and Endochondral Ossification of Human iPSC-Derived Chondrocytes. JBMR Plus 7:e10737 (2023). PubMed: 37197316
- Lin C et al. Increased expression of osteopontin in subchondral bone promotes bone turnover and remodeling, and accelerates the progression of OA in a mouse model. Aging (Albany NY) 14:253-271 (2022). PubMed: 34982732
- Jiang W et al. PGE2 activates EP4 in subchondral bone osteoclasts to regulate osteoarthritis. Bone Res 10:27 (2022). PubMed: 35260562
- Liu J et al. MiR-20a-5p facilitates cartilage repair in osteoarthritis via suppressing mitogen-activated protein kinase kinase kinase 2. Bioengineered 13:13801-13814 (2022). PubMed: 35707845
- Wu Z et al. Hsa_circ_0008870 suppresses bone formation of growth plate through inhibition of miR-185-3p/ MAPK1 axis in idiopathic short stature. Front Bioeng Biotechnol 10:1022830 (2022). PubMed: 36304901