Recombinant Anti-Collagen X antibody [EPR22920-211] (ab260040)
![Immunohistochemistry (Frozen sections) - Anti-Collagen X antibody [EPR22920-211] (ab260040)](https://www.abcam.com/ps/products/260/ab260040/Images/ab260040-1-anti-collagen-x-antibody-epr22920-211-immunohistochemistry-frozen-trachea-mouse.jpg "Immunohistochemistry (Frozen sections) - Anti-Collagen X antibody [EPR22920-211] (ab260040)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22920-211] to Collagen X
- Suitable for: IHC-Fr, IHC-P
- Reacts with: Mouse
Related conjugates and formulations
Overview
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Product name
Anti-Collagen X antibody [EPR22920-211]
See all Collagen X primary antibodies -
Description
Rabbit monoclonal [EPR22920-211] to Collagen X -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Fr, IHC-Pmore details
Unsuitable for: WB -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Mouse articular cartilage tissue. IHC-Fr: Mouse trachea tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22920-211 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab260040 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr |
1/100.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
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| IHC-P |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| Notes |
|---|
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IHC-Fr
1/100. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
|
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Type X collagen is a product of hyperthrophic chondrotocytes and has been localized to presumptive mineralization zones of hyaline cartilage. -
Involvement in disease
Defects in COL10A1 are the cause of Schmid type metaphyseal chondrodysplasia (SMCD) [MIM:156500]. SMCD is a dominantly inherited disorder of the osseous skeleton. The cardinal features of the phenotype are mild short stature, coxa vara and a waddling gait. Radiography usually shows sclerosis of the ribs, flaring of the metaphyses, and a wide irregular growth plate, especially of the knees. A variant form of SMCD is spondylometaphyseal dysplasia Japanese type. It is characterized by spinal involvement comprising mild platyspondyly, vertebral body abnormalities, and end-plate irregularity. -
Sequence similarities
Contains 1 C1q domain. -
Post-translational
modificationsProlines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 12813 Mouse
- SwissProt: Q05306 Mouse
- Unigene: 443177 Mouse
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Alternative names
- COAA1_HUMAN antibody
- Col10a 1 antibody
- COL10A1 antibody
see all
Images
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Immunohistochemistry (Frozen sections) - Anti-Collagen X antibody [EPR22920-211] (ab260040)
Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Trition X-100 permeabilized mouse trachea tissue labeling Collagen X with ab260040 at 1/100 dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (green). Positive staining on hyaline cartilage of mouse trachea (PMID: 22859705). The nuclear counterstain is DAPI (blue).
Secondary antibody only control: ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen X antibody [EPR22920-211] (ab260040)](https://www.abcam.com/ps/products/260/ab260040/Images/ab260040-2-anti-collagen-x-antibody-epr22920-211-immunohistochemistry-articular-cartilage-mouse.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen X antibody [EPR22920-211] (ab260040)Immunohistochemical analysis of paraffin-embedded mouse articular cartilage tissue labeling Collagen X with ab260040 at 1/1000 dilution (0.57µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Positive staining on the hypertrophic zone in the growth plate of mouse bone (PMID: 22859705). The section was incubated with ab260040 for 15 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: A ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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![Anti-Collagen X antibody [EPR22920-211] (ab260040)](https://www.abcam.com/ps/products/260/ab260040/Images/ab260040-3-benefits-of-recombinant-antibodies.png)
Anti-Collagen X antibody [EPR22920-211] (ab260040)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (4)
ab260040 has been referenced in 4 publications.
- Bao J et al. Pharmacological Disruption of Phosphorylated Eukaryotic Initiation Factor-2a/Activating Transcription Factor 4/Indian Hedgehog Protects Intervertebral Disc Degeneration via Reducing the Reactive Oxygen Species and Apoptosis of Nucleus Pulposus Cells. Front Cell Dev Biol 9:675486 (2021). PubMed: 34164397
- Leek CC et al. Deletion of Fibroblast growth factor 9 globally and in skeletal muscle results in enlarged tuberosities at sites of deltoid tendon attachments. Dev Dyn 250:1778-1795 (2021). PubMed: 34091985
- Colucci SC et al. Systemic Administration of Recombinant Irisin Accelerates Fracture Healing in Mice. Int J Mol Sci 22:N/A (2021). PubMed: 34639200
- Yajun W et al. Betaine Attenuates Osteoarthritis by Inhibiting Osteoclastogenesis and Angiogenesis in Subchondral Bone. Front Pharmacol 12:723988 (2021). PubMed: 34658862