Recombinant Anti-Collagen X antibody [EPR22920-211] - BSA and Azide free (ab264571)
![Immunohistochemistry (Frozen sections) - Anti-Collagen X antibody [EPR22920-211] - BSA and Azide free (ab264571)](https://www.abcam.com/ps/products/264/ab264571/Images/ab264571-1-anti-collagen-x-antibody-epr22920-211-immunohistochemistry-frozen-trachea-mouse.jpg "Immunohistochemistry (Frozen sections) - Anti-Collagen X antibody [EPR22920-211] - BSA and Azide free (ab264571)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22920-211] to Collagen X - BSA and Azide free
- Suitable for: IHC-P, IHC-Fr
- Reacts with: Mouse
Related conjugates and formulations
Overview
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Product name
Anti-Collagen X antibody [EPR22920-211] - BSA and Azide free
See all Collagen X primary antibodies -
Description
Rabbit monoclonal [EPR22920-211] to Collagen X - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, IHC-Frmore details
Unsuitable for: WB -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Mouse articular cartilage tissue. IHC-Fr: Mouse trachea tissue.
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General notes
ab264571 is the carrier-free version of ab260040.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22920-211 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab264571 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
| IHC-Fr |
Use at an assay dependent concentration.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
| Notes |
|---|
|
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
IHC-Fr
Use at an assay dependent concentration. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
Target
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Function
Type X collagen is a product of hyperthrophic chondrotocytes and has been localized to presumptive mineralization zones of hyaline cartilage. -
Involvement in disease
Defects in COL10A1 are the cause of Schmid type metaphyseal chondrodysplasia (SMCD) [MIM:156500]. SMCD is a dominantly inherited disorder of the osseous skeleton. The cardinal features of the phenotype are mild short stature, coxa vara and a waddling gait. Radiography usually shows sclerosis of the ribs, flaring of the metaphyses, and a wide irregular growth plate, especially of the knees. A variant form of SMCD is spondylometaphyseal dysplasia Japanese type. It is characterized by spinal involvement comprising mild platyspondyly, vertebral body abnormalities, and end-plate irregularity. -
Sequence similarities
Contains 1 C1q domain. -
Post-translational
modificationsProlines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 12813 Mouse
- SwissProt: Q05306 Mouse
- Unigene: 443177 Mouse
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Alternative names
- COAA1_HUMAN antibody
- Col10a 1 antibody
- COL10A1 antibody
see all
Images
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Immunohistochemistry (Frozen sections) - Anti-Collagen X antibody [EPR22920-211] - BSA and Azide free (ab264571)
Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Trition X-100 permeabilized mouse trachea tissue labeling Collagen X with ab260040 at 1/100 dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (green). Positive staining on hyaline cartilage of mouse trachea (PMID: 22859705). The nuclear counterstain is DAPI (blue).
Secondary antibody only control: ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab260040).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen X antibody [EPR22920-211] - BSA and Azide free (ab264571)](https://www.abcam.com/ps/products/264/ab264571/Images/ab264571-2-anti-collagen-x-antibody-epr22920-211-immunohistochemistry-articular-cartilage-mouse.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen X antibody [EPR22920-211] - BSA and Azide free (ab264571)Immunohistochemical analysis of paraffin-embedded mouse articular cartilage tissue labeling Collagen X with ab260040 at 1/1000 dilution (0.57µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Positive staining on the hypertrophic zone in the growth plate of mouse bone (PMID: 22859705). The section was incubated with ab260040 for 15 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: A ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab260040).
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![Anti-Collagen X antibody [EPR22920-211] - BSA and Azide free (ab264571)](https://www.abcam.com/ps/products/264/ab264571/Images/ab264571-3-benefits-of-recombinant-antibodies.png)
Anti-Collagen X antibody [EPR22920-211] - BSA and Azide free (ab264571)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab264571 has not yet been referenced specifically in any publications.
